ADSORPCJA LIZOZYMU – CHARAKTERYSTYKA STRUKTUR BIAŁKOWYCH Z ZASTOSOWANIEM METODY MIKROWAGI KWARCOWEJ Z MONITOROWANIEM DYSSYPACJI ENERGII ORAZ POWIERZCHNIOWEGO REZONANSU PLAZMONÓW
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Lysozyme (LSZ) exhibits strong antibacterial activity against gram-positive bacteria. This property has found practical applications in the medicinal and pharmaceutical industries. Elucidating protein orientation on a nanoscale surface has important implications for integrat- ing proteins into micro- and nano-fabricated devices. Analysis of conformational changes of proteins by quartz crystal microbalance (QCM-D) and surface plasmon resonance (MP-SPR) was performed. Both methods can be used to study protein adsorption phenomena (adsorbed amount and the thickness of protein layer). A combination of QCM-D and MP-SPR methods makes it possible to draw conclusions about the factors that control the interactions between LSZ and the surface of gold. pH strongly affects the effectiveness of LSZ adsorption onto the surface and leads to orientations changes of protein on the surface. The highest adsorption value was attained near the I.E.P. This data clearly indicates that electrostatic interactions are a driving force for LSZ adsorption and significantly affect the topography of layers formed on the surface. Furthermore, these meas- urements show high hydration (60%) of LSZ layer on surface of gold.
- 1. Deligianni D. D., Katsala N., Ladas S., Sotiropoulou D., Amedee J., Missirlis Y. F., Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell res- ponse and on protein adsorption, “Biomaterials” 2001, No. 22. 2. Huang Q., Huang P. M., Violante A. Soil Mineral Microbe-Organic Interactions: Theo- ries and Applications, Berlin, Heidelberg 2008. 3. Jachimska B., Kozłowska A., Pajor-Świerzy A., Protonation of Lysozymes and Its Con- sequences for the Adsorption onto a Mica Surface, “Langmuir” 2012, No. 28 (31). 4. Jachimska B., Wasilewska M., Adamczyk Z., Characterization of Globular Protein So- lutions by Dynamic Light Scattering, Electrophoretic Mobility, and Viscosity Mea- surements, “Langmuir” 2008, 24 (13). 5. Kubiak K., Mulheran P. A., Molecular Dynamics Simulations of Hen Egg White Ly- sozyme Adsorption at a Charged Solid Surface, “Journal of Physical Chemistry B” 2009, No. 113 (36). 6. Kubiak-Ossowska K., Mulheran P. A., Mechanism of Hen Egg White Lysozyme Adsorp- tion on a Charged Solid Surface, “Langmuir” 2010, No. 26 (20). 7. Marx K., Quartz Crystal Microbalance: A Useful Tool for Studying Thin Polymer Films and Complex Biomolecular Systems at the Solution-Surface Interface, “Biomacromole- cules” 2003, No. 4 (5). 8. Masel R .I., Principles of adsorption and reaction on solid surfaces, “Wiley Series in Chemical Engineering”, 1st edition, New York 1996. 9. Nezu T., Masuyama T., Sasaki K., Saitoh S., Taira M., Araki Y., Effect of pH and Ad- dition of Salt on the Adsorption Behavior of Lysozyme on Gold, Silica, and Titania Surfaces Observed by Quartz Crystal Microbalance with Dissipation Monitoring, “Dental Materials Journal” 2008, No. 27 (4). 10. Norde W., Horbett T. A., Brash J. L., Proteins at Interfaces III State of the Art, Washing- ton 2012. 11. Norde W., MacRitchie F., Nowicka G., Lyklema J., Protein adsorption at solid-liquid interfaces: Reversibility and conformation aspects, “Journal of Colloid and Interface science” 1986, No. 112 (2). 12. Sander M., Madliger M., Schwarzenbach R. P., Adsorption of transgenic insecticidal Cry1Ab protein to SiO2. 1. Forces driving adsorption, “Environmental Science Tech- nology” 2010, No. 44 (23). 13. Sener G., Ozgura E., Yılmaza E., Uzuna L., Sayc R., Denizlia A., Quartz crystal micro- balance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles, “Biosensors and Bioelectronics” 2010, nr 26 (2). 14. Sherwood J. D., Random sequential adsorption of lines and ellipses, “Journal of Physics A: Mathematical and General” 1990, No. 23 (13). 15. Ślósarek G., Badania właściwości fizyko-chemicznych lizozymu za pomocą metod fizycznych opartych na rozpraszaniu promieniowania, „Na pograniczu chemii i biologii” 2005, t. 13. 16. Xu K., Ouberai M. M., Welland M. E., A comprehensive study of lysozyme adsorption using dual polarization interferometry and quartz crystal microbalance with dissipation, “Biomaterials” 2012, No. 34 (5).
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