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2015 | 23 | 117-127
Article title

Micropropagation of Russelia equisetiformis Schlecht & Cham. through nodal segment culture

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EN
Abstracts
EN
An in vitro regeneration protocol of Russelia equisetiformis was established through nodal shoot segments cultured on Murashige and Skoog medium (MS) supplemented with different concentration and combination of cytokinins and auxins in the present study. Cent percent bud breaking response with maximum number of shoots (6.0) was observed on MS medium augmented with 1.0 mg/L 6-benzylaminopurine (BAP). Shoots multiplication further enhanced by repeated subculturing of in vitro shoots on MS medium with 1.0 mg/L BAP + 0.5 mg/L Kinetin (Kn) and additives. Maximum 13.2 shoots with 7.8 cm length were differentiated. Healthy shoots were rooted with high frequency (97%) on half strength MS medium containing 1.0 mg/L indole-3 butyric acid (IBA) with maximum number of roots (5.73). The in vitro regenerated plantlets were hardened in greenhouse and transferred to nursery with 85% survival rate.
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Year
Volume
23
Pages
117-127
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References
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Document Type
article
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YADDA identifier
bwmeta1.element.psjd-6348a6c0-fb19-4f59-96eb-fb5dfc360938
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