Cellulolytic Potentials of Fungal Isolates from Sawdust Environments

• Authors: P. O. Enakaye , K. A. Odelade , O. D. Umoren
• Languages of publication: EN

Abstracts

EN

Environmental wastes from agricultural activities are natural sources of bio-tools, particularly fungi of industrial importance for the synthesis of lignocellulytic enzymes. The aim of the study is to evaluate the cellulolytic potential of fungal isolates from sawdust environment. Sawdust samples were collected from sawmills and transported to the laboratory for processing. Fungal isolation and characterization, screening for Cellulase production, Fermentation Process for Cellulase Activity, and Screening of Cellulolytic Enzyme Activity were done using the zone of hydrolysis technique. A total of four cellulolytic isolates (Aspergillus carbonarius, Trichoderma harzianum, Aspergillus niger and Aspergillus flavus) was confirmed. All the isolates demonstrated cellulase production ability in the sawdust higher than the CMC, with Aspergillus carbonarius having the highest activity at 25 °C (35.00 mm) and 35 °C (39.00 mm) on sawdust than the CMC (30.00 mm) respectively. Furthermore, the cellulase production activity at 35 °C was higher than 25 °C. Sawdust is an excellent habitat for fungi that produce cellulase specifically at 25 °C. Thus, following more research, the acquired fungal species can be used to produce cellulase industrially.

Keywords

EN

Cellulolytic; fungi; sawdust environment

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2025
• Volume: 61
• Issue: 2
• Pages: 340-347

Contributors

author

P. O. Enakaye

• Department of Biological Sciences, National Open University of Nigeria, Abuja, Nigeria

author

K. A. Odelade

• Department of Science Laboratory Technology, Federal Polytechnic, Ayede, Oyo State, Nigeria

author

O. D. Umoren

• Department of Biological Sciences, National Open University of Nigeria, Abuja, Nigeria

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• Document Type: article