Antibiotic resistance of bacteria and incidence of carbapenamase-coding genes blaPER and blaGES in isolates from wastewater treatment plants
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The increase in number of antibiotic resistant bacteria poses a serious environmental and health problem. The development of microbial resistance is intensified by the widespread use of antibiotics in medicine, veterinary, farming and aquaculture. The wastewater treatment plants receiving the wastewater from hospitals, slaughterhouses, farms, pharmaceutical industry and houses can be potential places of spreading of antibiotic resistant genes. The molecular mechanisms of the bacterial resistance, including the horizontal transfer of antibiotic resistant genes and the presence of mobile genetic elements can be responsible for the increase in antibiotic resistance during wastewater treatment. The aim of this study was to analyze the phenomenon of bacterial resistance to selected β-lactam antibiotics (ESBL) by detecting the genes that determine this resistance. The PCR method was used to analyze the occurrence of two genes: blaPER and blaGES in wastewater samples. It was revealed that the resistant/total bacteria ratio was significantly higher in the effluent compared with the influent wastewater. Genes blaPER and blaGES were isolated from several strains predominating in both aeration tank (5% and 20% respectively) and effluent wastewater (15% and 12% respectively).
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