Micropropagation of Oldenlandia umbellata L. highly dye-yielding medicinal plant
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This paper proposes an efficient in vitro propagation method for a red dye yielding and medicinal herb - Oldenlandia umbellata L. The plant is well-known in Siddha medicine for its styptic property. It is also used as drug for bronchial asthma, as a febrifuge and to treat poisonous bites and ulcers. Moreover, the red dye from its roots has been used in diverse applications since ancient times. These varied applications have increased the utilization and exploitation of this plant for medicinal and dye extraction purposes. Hence, a protocol for rapid propagation is standardized in the present study. Herein, the nodal and internodal of Oldenlandia umbellata L. were cultured on MS medium supplemented with cytokinins, 6-Benzylaminopurine (BAP), GA3, Kinetin (KIN), and auxins 1-Naphthaleneacetic acid NAA, 2, 4-Dichlorophenoxyacetic Acid (2, 4-D). Direct organogenesis was the intent. In doing so, the nodal and internodal explants showed maximum number of shoots in the combination of 2.5 mg/l BAP, 2.0 mg/l GA3, 0.5 mg/l. In addition, the explants growing on medium supplemented with 10 mg/l BAP was found to have generated more shoots. The developed shoots were then transferred for rooting to half strength MS medium fortified with IAA, IBA and NAA ranging from 2-5 mg/l. The best results were achieved on 2.0 mg/l 89.6 % (IBA) Indole-3- butyric acid. For hardening, the rooted plantlets were subsequently transferred to paper cups containing FYM, red soil and sand in the ratio of 1:2:1. They were then transferred to the growth chamber for acclimatization. After acclimatization, the plantlets were transfer for further development under field conditions. About 95% survival was achieved in the field.
-  Seydel, P. and Dornenburg, H, Establishment of in vitro plants, cell and tissue cultures from Oldenlandia affinis for the production of cyclic peptides. Plant Cell Tiss. Org. Cult 85 (2006) 247-255.
-  Siva, R, Status of natural dyes and dye yielding plants in India. Curr. Sci 92 (2007) 916-925.
-  Gupta, M., Mazumder, U.K., Thamilselvan, V., Manikandan, L., Senthilkumar, G.P., Suresh, R.and Kakotti, B.K, Potential Hepatoprotective effect and antioxidant role of methanol extract of Oldenlandia umbellata in carbon tetrachloride induced hepatotoxicity in Wistar rats. Iran J. Pharmacol. Ther 6 (2007) 5-9.
-  Rekha S, Srinivasan V, Saradha V, Hamsaveni G, The in vitro antibacterial activity of Hedyotis umbellata. Indian J Pharm Sci 68 (2006) 2368.
-  Siva R, Mudgal G, Rajesh D, Khan FN, Vijyakumar V, Rajasekaran C, Characterization of novel pH indicator of natural dye Oldenlandia umbellata L. Nat Prod Res 23 (2009) 1210-1217.
-  Siva R, Rajasekaran C, Mudgal G, Induction of somatic embryogenesis and organogenesis in Oldenlandia umbellata L., a dye-yielding medicinal plant. Plant Cell Tiss Org Cult 98 (2009) 205-211.
-  Siva R, Mayes S, Behera SK, Rajasekaran C, Anthraquinones dye production using root cultures of Oldenlandia umbellata L. Ind Crop Prod 37(1) (2012) 415-419.
-  Shekhawat MS, Kannan N, Manokari M, Revathi J, In vitro propagation of Oldenlandia umbellata L. - a highly medicinal & dye-yielding plant of Coromandel Coast. Int J Recent Sci Res 3 (2012) 758-761.
-  Shekhawat MS, Kannan N, Manokari M, Revathi J, Induction of shoots from in vitro cultured roots of Oldenlandia umbellata L.-a dye yielding plant. Res J Pharmacogn Phytochem 5 (2013) 22-25.
-  Rao GP, Bahadur B, Somatic embryogenesis and plant regeneration in self-incompatible Oldenlandia umbellata L. (Rubiaceae). Phytomorphology 40 (1990) 1-2.
-  Valizadeh J, Valizadeh M, In vitro callus induction and plant regeneration from Withania coagulans: a valuable medicinal plant. Pak J Biol Sc 12 (2009) 1415-1419.
-  Benesi IRM, Labuschagne MT, Herselman L, Mahungu N, Ethnobotany, morphology and genotyping of cassava germplasm from Malawi. J Bio Sc 7 (2010) 616-623.
-  Sultana RS, Bari Miah MA, In vitro propagation of Karella (Momordica charantea L.) from nodal segment and shoot tip. J Biol Sc 3 (2003) 1134-1139.
-  Murashige T, Skoog F, A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15 (1962) 473-497.
-  Martinussen, I., Nilsen, G., Svenson, L., Junttila, O. and Rapp, K, In vitro propagation of cloudberry (Rubus chamaemorus). Plant Cell Tiss. Org. Cult 78 (2004) 43-49.
-  Velayutham, P., Ranjithakumari, B.D. and Baskaran, P, An effeicient in vitro plant regeneration system for Cichorium intybus L. an important medicinal plant. Journal Agri. Tech 2(2) (2006) 287-298.
-  Padmapriya, H., Karthikeyan, A.V.P., Jahir Hussain, G., Karthi, C. and Velayutham P, An efficient protocol for in vitro propagation of Solanum nigrum L. from nodal explants. Journal of Agricultural Technology 7(4) (2011) 1063-1073.
-  Sweety, M., Animesh, B. and Mahbubur, R, In vitro mass propagation of Gunura procumbens (LOUR.) MERR.-An important medicinal pant. Asian Journal of Natural & Applied Sciences 5(3) (2016) 71-79.
-  Manokari, M., Ravindran, C.P. and Shekhawat, M.S, Optimization of in vitro regeneration in Hibiscus subdariffa L.-An important medicinal plant. World Scientific News 34 (2016) 51-62.
-  Selvaraj, N., Vasudevan, A., Manickavasagam, M. and Ganapathi, A, In vitro organogenesis and plant formation in cucumber. Biol. Plant 50 (2006) 123-126.
-  Baskaran, P., Velayutham, P., Rajadurai, D.M. and Jayabalan, N, Micropropagation of Melothria maderaspatana (L) Cogn. through apical buds derived from mature plants. J. Biol. Res.-Thessalon 9 (2008) 83-88.
-  Sija, S.L., Potty, V.P. and Santhoshlal, P.S, In Vitro Shoot Proliferation from Excised Shoot Tip and Nodal Segment of Anacardium occidentale L. Int. J. Curr. Microbiol. App. Sci 5(3) (2016) 635-642.
-  Murch, S.J. Krishna, R. S & Saxena, P.K, Tryptophan as a precursor for melatonin and serotonin biosynthesis in in-vitro regenerated St. John's ort (Hypericum perforatum. cv. Anthos) lants. Plant Cell Rep 19 (2000) 698-704.
-  Pattnaik, S.K. & Cland, P.K, Rapid clonal propagation of three mulberries, Morus cathayana Hemsl., M. ihou Koiz. and M. serrata Roxb., through in vitro culture of apical shoot buds and nodal explants from mature trees. Plant Cell Rep 16 (1997) 503- 508.
-  Vijaya Chitra, D.S. & Padmaja, G, Clonal propagation of mulberry (Morus indica L. cultivar M5) through in vitro culture of nodal explants. Sci. Hortic 80 (1999) 289-298.
-  Bharat Singh Rajput, Minal D. Jani, K. Sasikumar, M. Manokari, M. S. Shekhawat. An Improved Micropropagation Protocol for Manga Bamboo – Pseudoxytenanthera stocksii (Munro) T.Q. Nguyen. World News of Natural Sciences 25 (2019) 141-154
-  Saranya Krishnan, S.R. & Siril, E.A. Enhanced In Vitro Shoot Regeneration in Oldenlandia umbellata L. by Using Quercetin: A Naturally Occurring Auxin-Transport Inhibitor. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. (2017) 87: 899. https://doi.org/10.1007/s40011-015-0672-0
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