Bombesin-like peptides are able to affect central histamine-induced resuscitating effect in haemorrhage-shocked rats

• Authors: Karolina Jasikowska , Barbara Rybus-Kalinowska , Aleksandra Klose , Damian Nowak , Jerzy Jochem

Title variants

PL

Peptydy bombezynopodobne mogą wpływać na efekt resuscytacyjny ośrodkowo działającej histaminy we wstrząsie krwotocznym u szczurów

• Languages of publication: EN PL

Abstracts

EN

INTRODUCTION: Activation of the central histaminergic system induces a resuscitating effect in haemorrhage-shocked rats. Since peripherally administered bombesin evokes a similar action, and bombesin receptors are present in the central nervous system regions responsible for cardiovascular regulation, the aim of the study was to examine the effects of bombesin receptor blockage on histamine-induced cardiovascular effects in haemorrhagic shock. MATERIAL AND METHODS: Studies were carried out in male Wistar rats anaesthetized with ketamine/xylazine (100 mg/kg + 10 mg/kg, intraperitoneally) and then subjected to reversible haemorrhagic shock with a mean arterial pressure (MAP) of 30–35 mmHg. Immediately after terminating bleeding, the animals were pre-treated intracerebroventricularly (icv) with bombesin receptor antagonists/0.9% NaCl solution, and 5 min later – treated via the same route with histamine (50 nmol) or 0.9% NaCl solution. RESULTS: Haemorrhage led to decreases in the pulse pressure (PP), heart rate (HR) and mesenteric blood flow (MBF). Histamine induced a pressor effect, with a significant increase in PP and MBF. The effect was inhibited by both [D-Phe12,Leu14]-bombesin and BIM 23042, non-selective and selective bombesin 1 (BB1) receptor antagonists, respectively. In the control groups the antagonists had no effect. CONCLUSIONS: Endogenous bombesin-like peptides, acting via BB1 receptors, are able to affect a histamine-induced pressor effect in haemorrhage-shocked rats.

PL

WSTĘP: Pobudzenie ośrodkowego układu histaminergicznego wywołuje działanie resuscytacyjne u szczurów we wstrząsie krwotocznym. Skoro podawana dożylnie bombezyna wykazuje podobne działanie, a receptory bombezynowe są obecne w strukturach ośrodkowego układu nerwowego, odpowiedzialnych za regulację czynności układu krążenia, celem pracy było zbadanie wpływu zablokowania receptorów bombezynowych na efekty krążeniowe wynikające z podania do komory bocznej mózgu (icv) histaminy we wstrząsie krwotocznym u szczurów. MATERIAŁ I METODY: Badania przeprowadzono u szczurów, samców szczepu Wistar w znieczuleniu ogólnym (ketamina 100 mg/kg + ksylazyna 10 mg/kg, dootrzewnowo). Zastosowano model odwracalnego wstrząsu krwotocznego ze średnim ciśnieniem tętniczym (MAP) 30–35 mmHg. Niezwłocznie po zakończeniu krwawienia podawano icv antagonistów receptorów bombezynowych/0,9% roztwór NaCl, a 5 min później tą samą drogą histaminę (50 nmol) bądź 0,9% roztwór NaCl. WYNIKI: Utrata krwi prowadziła do obniżenia ciśnienia tętna (PP), częstości rytmu serca (HR) i przepływu krezkowego krwi (MBF). Histamina wywoływała działanie presyjne ze wzrostem PP i MBF. Efekty działania histaminy były częściowo hamowane przez [D-Phe12,Leu14]-bombezynę i BIM 23042, odpowiednio nieselektywnego antagonistę receptorów bombezynowych oraz selektywnego blokera receptorów bombezynowych typu 1 (BB1). W grupach kontrolnych nie stwierdzono wpływu antagonistów receptorów bombezynowych na mierzone parametry układu krążenia. WNIOSKI: Endogenne peptydy bombezynopodobne, działając poprzez receptory BB1, mogą wpływać na wywoływane przez ośrodkowo działającą histaminę działanie resuscytacyjne we wstrząsie krwotocznym u szczurów.

Keywords

EN

bombesin receptors; haemorrhagic shock; histaminergic system; rats; receptory bombezynowe; szczury; układ histaminergiczny; wstrząs krwotoczny

Discipline

• MEDICINE: MEDICINE

• Publisher: Śląski Uniwersytet Medyczny w Katowicach
• Journal: Annales Academiae Medicae Silesiensis
• Year: 2020
• Issue: 74
• Pages: 40-45

Contributors

author

Karolina Jasikowska

• Department of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland

author

Barbara Rybus-Kalinowska

• Department of Basic Medical Sciences, Faculty of Health Sciences in Bytom, Medical University of Silesia, Katowice, Poland

author

Aleksandra Klose

• Department of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland

author

Damian Nowak

• Department of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland

author

Jerzy Jochem

• Department of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland

References

• 1. Kauvar D.S., Lefering R., Wade C.E. Impact of hemorrhage on trauma outcome: an overview of epidemiology, clincal presentations, and therapeutic conside-rations. J. Trauma 2006; 60: S3–S11, doi: 10.1097/01.ta.0000199961.02677.19.
• 2. Barcroft H., McMichael J., Edholm O.G., Sharpey-Schafer E.P. Posthaemorrhagic fainting: Study by cardiac output and forearm flow. Lancet 1944; 1: 489–491.
• 3. Jacobsen J., Secher N.H. Heart rate during haemorrhagic shock. Clin. Physiol. 1992; 12(6): 659–666, doi: 10.1111/j.1475-097x.1992.tb00369.x.
• 4. Huang H., Li Y., Liang J., Finkelman F.D. Molecular regulation of histamine synthesis. Front Immunol. 2018; 9: 1392, doi: 10.3389/fimmu.2018.01392.
• 5. Brown R.E., Stevens D.R., Haas H.L. The physiology of brain histamine. Prog. Neurobiol. 2001; 63(6): 637–672, doi: 10.1016/s0301-0082(00)00039-3.
• 6. Wada H., Inagaki N., Yamatodani A., Watanabe T. Is the histaminergic neuron system a regulatory center for whole-brain activity? Trends Neurosci. 1991; 14(9): 415–418, doi: 10.1016/0166-2236(91)90034-r.
• 7. Bealer S.L. Central neuronal histamine contributes to cardiovascular regulation. News Physiol. Sci. 1999; 14: 100–105, doi: 10.1152/physiologyonline.1999.14.3.100.
• 8. Jochem J. Cardiovascular effects of histamine administered intracerebroventricularly in critical haemorrhagic hypotension in rats. J. Physiol. Pharmacol. 2000; 51(2): 229–239.
• 9. Jochem J. Central histamine-induced reversal of critical haemorrhagic hypotension in rats – haemodynamic studies. J. Physiol. Pharmacol. 2002; 53(1): 75–84.
• 10. Jochem J. Haematological, blood gas and acid-base effects of central histamine-induced reversal of critical haemorrhagic hypotension in rats. J. Physiol. Pharmacol. 2001; 52(3): 447–458.
• 11. Jochem J., Kasperska-Zając A. The role of the histaminergic system in the central cardiovascular regulation in haemorrhagic hypotension. Folia Med. Cracov. 2012; 52(3-4): 31–41.
• 12. Anastasi A., Erspamer V., Bucci M. Isolation and structure of bombesin and alytesin, 2 analogous active peptides from the skin of the European amphi-bians Bombina and Alytes. Experientia. 1971; 27(2): 166–167, doi: 10.1007/bf02145873.
• 13. Jensen R.T., Battey J.F., Spindel E.R., Benya R.V. International Union of Pharmacology. LXVIII. Mammalian bombesin receptors: nomenclature, distribution, pharmacology, signaling, and functions in normal and disease states. Pharmacol. Rev. 2008; 60(1): 1–42, doi: 10.1124/pr.107.07108.
• 14. Moody T.W., Merali Z. Bombesin-like peptides and associated receptors within the brain: distribution and behavioral implications. Peptides 2004; 25(3): 511–520, doi: 10.1016/j.peptides.2004.02.012.
• 15. Li P., Janczewski W.A., Yackle K., Kam K., Pagliardini S., Krasnow M.A., Feldman J.L. The peptidergic control circuit for sighing. Nature 2016; 530(7590): 293–297, doi: 10.1038/nature16964.
• 16. Guarini S., Tagliavini S., Bazzani C., Bertolini A. Bombesin reverses bleeding-induced hypovolemic shock, in rats. Life Sci. 1989; 45(2): 107–116, doi: 10.1016/0024-3205(89)90284-1.
• 17. Jasikowska K., Czuba Z., Jochem J. Effects of histamine H4 receptor ligands in hemorrhagic shock in rats. 47th Annual Meeting European Histamine Research Society, Dublin 30.05-02.06.2018, Abstract book: p. 66.
• 18. Bertolini A. The opioid/anti-opioid balance in shock: a new target for therapy in resuscitation. Resuscitation 1995; 30(1): 29–42, doi: 10.1016/0300-9572(94)00863-b.
• 19. Jochem J., Zwirska-Korczala K. Involvement of central noradrenergic system in the pressor effect of histamine administered intracerebroventricularly in rats – haemodynamic studies. Inflamm. Res. 2002; 51(Suppl 1): S59–S60, doi: 10.1007/pl00022448.
• 20. Jochem J., Zak A., Rybczyk R., Irman-Florjanc T. Interactions between the serotonergic and histaminergic systems in the central cardiovascular regulation in haemorrhage-shocked rats: involvement of 5-HT1A receptors. Inflamm. Res. 2009; 58(Suppl 1): S38–S40, doi: 10.1007/s00011-009-0658-6.
• 21. Jochem J., Savci V., Filiz N., Rybus-Kalinowska B., Fogel W.A., Yalcin M. Involvement of the histaminergic system in cytidine 5'-diphosphocholine-induced reversal of critical haemorrhagic hypotension in rats. J. Physiol. Pharmacol. 2010; 61(1): 37–43.
• 22. Jochem J., Zwirska-Korczala K., Sowa P., Berdowska A. Interactions between the histaminergic and angiotensinergic systems in the central cardiovascular regulation in rats. Inflamm. Res. 2006; 55(Suppl 1): S69–S70, doi: 10.1007/s00011-005-0047-8.
• 23. Fisher L.A., Cave C.R., Brown M.R. Central nervous system cardiovascular effects of bombesin in conscious rats. Am. J. Physiol. 1985; 248(4 Pt 2): H425–H431, doi: 10.1152/ajpheart.1985.248.4.H425.
• 24. Le Mével J.C., Lancien F., Mimassi N., Kermorgant M., Conlon J.M. Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout. Biol. Open. 2013; 2(9): 960–967, doi: 10.1242/bio.20135553.
• 25. Pinto I.S., Mourão A.A., da Silva E.F., Camargo A.S., Marques S.M., Gomes K.P., Fajemiroye J.O., da Silva Reis A.A., Rebelo A.C.S., Ferreira-Neto M.L., Rosa D.A., Freiria-Oliveira A.H., Castro C.H., Colombari E., Colugnati D.B., Pedrino G.R. Blockade of rostral ventrolateral medulla (RVLM) bombesin receptor type 1 decreases blood pressure and sympathetic activity in anesthetized spontaneously hypertensive rats. Front Physiol. 2016; 7: 205, doi: 10.3389/fphys.2016.00205.
• 26. Lateef D.M., Xiao C., Brychta R.J., Diedrich A., Schnermann J., Reitman M.L. Bombesin-like receptor 3 regulates blood pressure and heart rate via a central sympathetic mechanism. Am. J. Physiol. Heart Circ. Physiol. 2016; 310(7): H891–H898, doi: 10.1152/ajpheart.00963.2015.
• 27. Zogovic B., Pilowsky P.M. Intrathecal bombesin is sympathoexcitatory and pressor in rat. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2011; 301(5): R1486–R1494, doi: 10.1152/ajpregu.00297.2011.
• 28. Kaczyńska K., Szereda-Przestaszewska M. Vasopressor and heart rate responses to systemic administration of bombesin in anesthetized rats. Pharmacol. Rep. 2011; 63(2): 448–454, doi: 10.1016/s1734-1140(11)70511-4.
• 29. Okuma Y., Yokotani K., Murakami Y., Osumi Y. Brain histamine mediates the bombesin-induced central activation of sympatho-adrenomedullary outflow. Life Sci. 1997; 61(26): 2521–2528, doi: 10.1016/s0024-3205(97)01006-0.
• 30. Jochem J. Involvement of the sympathetic nervous system in the reversal of critical haemorrhagic hypotension by endogenous central histamine in rats. Naunyn Schmiedebergs Arch. Pharmacol. 2004; 369(4): 418–427, doi: 10.1007/s00210-004-0883-z.

• Document Type: article