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2019 | 130 | 238-249
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Effective and Large Scale In vitro Propagation of Dendrocalamus strictus (Roxb.) Nees using Nodal Segments as Explants

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Dendrocalamus strictus (Roxb.) Nees belongs to the family Poaceae is the most important and fastest growing bamboo species. An effort has been made to propagate D. strictus using nodal explants selected from mature mother plants. The disinfected nodes were inoculated on agar gelled Murashige and Skoog’s (MS) basal medium with various concentrations of 6-benzylaminopurine (BAP), while maximum 4-5 shoots were induced on 4.0 mg/l BAP. The newly formed buds were transferred to solidified MS basal medium containing 3.0 mg/l BAP and 0.5 α-Naphthalene acetic acid (NAA) to achieve about 26 multiple shoots. These experiments were conducted with a cluster of minimum 3-5 shoots. The induction of roots from in vitro regenerated shoots was found difficult in this plant system. Three types of auxins i.e. indole-3 butyric acid (IBA), indole-3 acetic acid (IAA) and NAA were used in various combinations for rooting of shoots. About 93 % rooting was archived on half strength MS medium augmented with 3.0 mg/l NAA. The rooted shoots were hardened in the greenhouse for two months and the secondary hardened plantlets were successfully shifted to the farmer’s field. More than 90 % survival rate was observed under the natural conditions in this plant species.
Physical description
  • Prem Sarita AgriBiotech, Rajpipla, Dist. Narmada, Gujarat, India
  • Tissue Culture Lab, Silviculture and Forest Utilization, Research Range, Rajpipla, Dist. Narmada, Gujarat, India
  • Tissue Culture Lab, Silviculture and Forest Utilization, Research Range, Rajpipla, Dist. Narmada, Gujarat, India
  • Biotechnology Laboratory, K. M. Centre for P. G. Studies, Puducherry - 605 008, India
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