Germinability of soybean seeds stored more than 30 years in the Bulgarian national seed genebank
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The maintenance of seed viability over long periods of time in genebanks is a key element in conservation of plant genetic resources. In this work, data obtained at the Bulgarian National seed Genebank from the routine task of monitoring seed viability from soybean were analyzed. Differences between initial, first and second germination test after 11 and 34 years of long-term storage were assessed among 182 accessions from Glycine max (L.) Merr. The samples were regenerated during 1978-1981 growing seasons. On the basis of experimental data, the seed storage characters - σ, P50% and P10% were determined allowing the prediction of seed storage life and the regeneration needs. Significant decrease in seed viability after 11 years of storage was not recorded in the investigated soybean accessions from the different regeneration years. When comparing the initial germination percentages with results from the second control test after 34 years of storage were found significant differences in the loss of seed viability among genotypes from different regeneration years. The frequency of odd results were most significant for genotypes regenerated in 1981 (0.691) in comparison with these from other regeneration seasons. The highest Ki value (2.09) was recorded for accessions from 1981 harvest year; where rate of seed deterioration (1/σ) was also highest (-0.044). The lowest values both for Ki and 1/σ were found for the groups of accessions harvested in 1978 and 1979. There was a wide variation between groups of accessions regenerated in different years in both the time taken for viability to fall to 50% and the time for seed viability reduction with 10%. The predicted mean safe storage time for Glycine max (L.) Merr. was 25.54 years. Total 76 accessions or 42,2% of analyzed accessions had significant decrease of germination after 34 years of storage and would need regeneration.
- Department of Plant Genetic Resources, Institute of Plant Genetic Resources “Konstantin Malkov”, 2 Druzhba Str., 4122 Sadovo, Bulgaria, email@example.com
- Department of Plant Genetic Resources, Institute of Plant Genetic Resources “Konstantin Malkov”, 2 Druzhba Str., 4122 Sadovo, Bulgaria
- Department of Plant Genetic Resources, Institute of Plant Genetic Resources “Konstantin Malkov”, 2 Druzhba Str., 4122 Sadovo, Bulgaria
-  J. Singh, S. Paroha, R. P. Mishra. Effect of Storage on Germination and Viability of Soybean (Glycine max) and Niger (Guizotia abyssinica) Seeds. International Journal of Current Microbiology and Applied Sciences 5 (7) (2016) 484-491.
-  A. Odoba, N. I. Odiaka, A. U. Gbanguba, M. Bashiru. Germination characteristics of twenty varieties of soybean (Glycine max (L.) Merr) stored for seven months. Scientia Agriculturae 13 (3) (2016) 151-155.
-  P. I. Akubor, M. U. Ukwuru. Functional properties and biscuit making potential of soybean and cassava flour blends. Plant Foods for Human Nutrition 58(3) (2005) 1-12.
-  P. Gobinath, P. Pavadai. Effect of gamma rays on morphology, growth, yield and biochemical analysis in soybean (Glycine max (L.) Merr.). World Scientific News 23 (2015) 1-12.
-  Y. B. Fu, Z. Ahmed, A. Diederichsen. Towards a better monitoring of seed ageing under ex situ seed conservation. Conserv. Physiol. 3 (2015) 1-16
-  FAO, Genebank Standards. 1994. Food and Agriculture Organization of the United Nations, Rome, International Plant Genetic Resources Institute, Rome, 1994, Available at ftp://ftp.fao.org/docrep/fao/meeting/015/aj680e.pdf.
-  C. Walters. Understanding the mechanisms and kinetics of seed aging. Seed Science Research 8 (1998) 223-244.
-  FAO, Genebank standards for plant genetic re¬sources for food and agriculture, Rome, 2014, Available at http://www.fao.org/3/a-i3704e.pdf.
-  V. R. Shelar. Strategies to Improve the Seed Quality and Storability of Soybean – A Review, Seed Technology Research Unit (NSP), Mathtme Phule Krighi Vidyprth, Rahuri 413-722, India, Agricultural Review 28 (3) (2007) 188-196.
-  V. R. Shelar, R. S. Shaikh, A. S. Nikam. Soybean seed quality during Storage: A Review, Agric. Rev. 29 (2) (2008) 125-131.
-  O. T. Isaac, E. A. Seweh, S. Apuri, B. K. Banful, S. Amoah. Effect of storage periods on seed quality characteristics of three soybean (Glycine max (L) Merrill) varieties. International Journal of Scientific Research in Science, Engineering and Technology, 2 (4) (2016a) 823-831.
-  M. Kundu, J. Kachari. Desication sensitivity and recalcitrant behavior of seeds Aquilariaagallocha Roxb. Seed Science Technology 28 (2000) 755-760.
-  C. Walters, L. M. Hill, L. J. Wheeler. Dying while dry: kinetics and mechanisms of dete-rioration in desiccated organisms. Integrative and Comparative Biology, 45 (2005) 751-758.
-  A. A. Sajo, V. T. Tame. Effects of storage materials and environmental conditions on time of germination of soybean (Glycine max (L.) Merril) seed in Yola, Nigeria. Acta Horticulture 1012, 1 (2012) 71-76.
-  S. Tang, D. M. Tekrony, D. B. Egli, P. L. Cornelius. An alternative model to predict corn seed deterioration during storage. Crop Sci. 40 (2000) 463-470.
-  F. R. Hay, A. Mead, K. Manger, F. J. Wilson. One-step analysis of seed storage data and the longevity of Arabidopsis thaliana seeds. Journal of Experimental Botany 54 (2003) 993-1011.
-  A. F., Mollah, M. M. Haque, S. M. M., Ali, A. T. M. M. Alam, A. B. Siddique, M. G. Mostofa. Quality evaluation of Jute seeds collected from different sources. Journal of Biological Sciences 2(7) (2002) 477-480.
-  M. Sacande, J. Buitink, F. A. Hoekstra. A study of water relations in Neem (Azadiractaindica) seed that is characterized by complex storage behavior. Journal of Experimental Botany 51(344) (2000) 635-643.
-  L. G. Heatherly, R. W. Elmore, Managing inputs for peak production. In: Soybeans: Improvement, Production and Uses, Eds- Boerma H.R., Specht, J.E. 3rd Edition, Agronomy N-16, ASA, CSSA, SSSA, Madison, Wisconsin, USA, (2004) 451-536.
-  S. D. Stoyanova. Ex situ conservation in the Bulgarian genebank: I. Effect of storage. PGR Newsletter 128 (2001) 68-76.
-  C. Walters, L. M. Wheeler, J. M. Grotenhuis. Longevity of seeds stored in a genebank: species characteristics. Seed Science Research 15 (2005) 1-20.
-  M. Nagel, H. Vogel, S. Landjeva, G. Buck-Sorlin, U. Lohwasser, U. Scholz, A. Börner. Seed conservation in ex situ genebanks – genetic studies on longevity in barley. Euphytica 170 (2009) 5-14.
-  R. J. Probert, M. I. Daws, F. R. Hay. Ecological correlates of ex situ seed longevity: a comparative study on 195 species. Annals of Botany 104 (2009) 57-69.
-  R. van Treuren, E. C. de Groot, J. L. van Hintum. Preservation of seed viability during 25 years of storage under standard genebank conditions. Genetic Resources Crop Evaluation 60 (2013) 1407-1421.
-  G. Desheva. The longevity of crop seeds stored under long-term condition in the national gene bank of Bulgaria. Agriculture (Poľnohospodárstvo) 62(3) (2016) 90-100.
-  J. M. M. Engels, L. Visser. A guide to effective management of germplasm collections. International Plant Genetic Resources Institute Handbook for Genebanks No 6, International Plant Genetic Resources Institute, Rome, Italy, (2003).
-  ISTA, International rules for seed testing. Seed Science and Technology 21 (Supplement), (1993) 1-288.
-  R. H. Ellis, T. D. Hong, E. H. Roberts, Handbooks for genebanks 2, Handbook of seed technology for genebanks Vol. I: Principles and methodology, Rome, Italy: International Boards for Plant Genetic Resources, (1985) pp 210.
-  R. H. Ellis, T. D. Hong, E. H. Roberts, Handbooks for genebanks 3, Handbook of seed technology for genebanks, Vol. II: Compendium of specific germination information and test recommendations. Rome, Italy: International Boards for Plant Genetic Resources, (1985) pp 456.
-  J. Hanson, Practical Manuals for Genebanks, IBPGR, Rome (1985), pp. 115.
-  E. H. Roberts. Predicting the storage life of seeds. Seed Science and Technology, 1 (1973) 499-514.
-  R. H. Ellis, E. H. Roberts. Improved equations for the prediction of seed longevity. Annals of Botany 45 (1980) 13-30.
-  D. V. S. S. R Sastry, H. D. Upadhyaya, C. L. L. Gowda. Seed viability of active collections in ex-situ genebanks: an analysis of sorghum germplasm conserved at ICRISAT genebank. Journal of SAT Agricultural Research 6 (2008) 1-8.
-  H. K. Parzies, W. Spoor, R. A. Ennos. Genetic diversity of barley landrace accessions (Hordeum vulgare ssp. vulgare) conserved for different lengths of time in ex situ gene banks. Heredity 84 (2000) 476-486.
-  E. H. Roberts, R. H. Ellis. Physiological, ultra structural and metabolic aspects of seed viability, In A. A. Khan, (Ed) The Physiology and biochemistry of seed development dormancy and germination, Amsterdam, Elsevier Biomedical Press, (1982) 465-485.
-  M. Benková, M. Žáková. Seeds germinability of selected species after five and ten years storage at different temperatures. Agriculture (Poľnohospodárstvo) 55 (2) (2009) 119-124.
-  S. K. Agha, Z. H. Malik, M. Hatam, G. H. Jamro. Emergence of healthy seedlings of soybean as influenced by seed storage containers. Pakistan Journal of Biological Sciences 7 (1) (2004) 42-44.
-  C. Reuzeau, G. Cavalie. Activities of free radical processing enzymes in dry sunfl ower seeds. New Phytol. 130 (1995) 59-66.
-  S. E. Trawatha, D. M. TeKrony, D. F. Hildebrand. Relationship on soybean quality to fatty acid and C6-aldehyde levels during storage. Crop Sci. 35 (1995) 1415-1422.
-  S. Balašević-Tubić, Đ. Malenčić, M. Tatić, J. Miladinović. Influence of aging process on biochemical changes in sunflower seed. Helia 28 (42) (2005) 107-114.
-  M. B. Kurdikeri, M. N. Merwade. Channaveeraswamy, Maintenance of viability in different crop species under ambient storage. Seed Res. 28 (2000) 109-110.
-  M. Ruiz, I. Martin, C. Cuadra. Cereal seed viability after 10 years of storage in active and base germplasm collections. Field Crops Research, 64 (1999) 229-236.
-  L. O. Copeland, M. B. McDonald. Principles of seed science and technology, 4th. Massachusetts, USA: Kluwer Academic Publishers, (2001) 190-230.
-  [S. Stoyanova. Conservation of peanut germplasm in the Bulgarian Genebank, Arachis genetic resources in Europe, Ad hoc meeting, 15-16 November 2002, Plovdiv, Bulgaria, International Plant Genetic Resources Institute, Rome, Italy (2004) 11-16.
-  X. Chen, X. Lu, C. Cui, H. Chen, H. Wang. Monitoring of sorghum (Sorghum vulgare) seed viability after a decade of storage in the National Genebank of China. PGR Newsletter 136 (2003) 54-57.
-  F. Pérez-García, C. Gómez-Campo, R. H. Ellis. Successful long-term ultra-dry storage of seed of 15 species of Brassicaceae in a genebank: variation in ability to germinate over 40 years and dormancy. Seed Science and Technology 37(3) (2009) 640-649.
-  D. G. F Walsh, S. Waldren, J. R. Martin. Monitoring seed viability of fifteen species after stor¬age in the Irish threatened plant genebank. Biology and Environment: Proceedings of The Royal Irish Academy 103B (2) (2003) 59-67.
-  S. Stoyanova. Effect of seed drying on seed viability under long-term storage conditions in the Bulgarian National Genebank. Agricultural Sciences 2(3) (2010) 33-38.
-  X. Lu, X. Chen, C. Cui. Germination ability of seeds of 23 crop plant species after a decade of storage in the National Gene Bank of China. Plant Genet Resources Newsletter 139 (2004) 42-46.
-  K. G. Sai Babu, S. H. Husaiai, R. B. Muralimonan. Effect of moisture and container on the storability of paddy seed under ambient conditions of Hyderabad. Seed Research 11 (1) (1983) 71-73.
-  V. R. Shelar, N. Shaikh. Soybean seed quality during storage. Seed Technology research, Agricultural Review 29 (2) (2002) 125-131.
-  P. Pratt, P. Bolin, C. Godsey, (Eds.). Soybean Production Guide, Oklahoma Cooperative Extension Service, Division of Agricultural Sciences and Natural Resources, Oklahoma State University, (2009) 12-114.
-  N. Khaliliaqdam, A. Soltani, N. Latifi, F. G. Far. Quantitative response of soybean seed under controlled conditions. American –Eurasian J. Agric. and Environ. Sci. 12(2) (2012) 224-230.
-  H. Kandel, (Ed.). Soybean Production. North Dakota State University, Fargo, Norht Dakota (2010) 128-142.
-  M. Tatic, S. Balesevic- Tubic, V. Dordevic, V. Miklic, M. Vujakovic, V. Dukic. Vigor of sunflower and soybean ageing seed. Helia 35(56) (2012) 119-126.
-  Ð. Malenčić, M. Popović, J. Miladinović. Stress tolerance parameters in different genotypes of soybean. Biology Plantarum 46 (2003) 141-143.
-  P. C. Gupta. Viability of stored soybeans seeds in India. Seed Res. 4 (1) (1976) 32-39.
-  L. O. Copeland, B. M. McDonald. Seed viability testing. In principles of seed science and technology (3rdEd), Chapman and Hall, New York, (1995) 111-126.
-  M. Nagell, M. A. R. Arif, M. Rosenhauer, A. Börner. Longevity of seeds ‒ intraspecific differences in the Gatersleben genebank collections. Tagung der Vereinigung der Pflanzenzüchter und Saatgutkauflfleute Österreichs (2010) 179-182.
-  S. Balešević-Tubić, M. Tatić, V. Ðorđević, Z. Nikolić, V. Ðukić. Seed viability of oil crops depending on storage conditions. Helia 33(52) (2010) 153-160.
-  M. Kausar, T. Mahmood, S. M. A. Basra, M. Arshad. Invigoration of low vigor sunflower hybrids by seed priming. Int. J. Agric. Biol. 11 (2009) 521-528.
-  R. K. Singh, R. K. Raipuria, V. S. Bhatia, A. Rani, Pushpendra, S. M. Husain, D. Chauhan, G. S. Chauhan, T. Mohopatra. SSR markers associated with seed longevity in soybean. Seed Science Technology 36 (2008) 162-167.
-  R. K. Singh, R. K. Raipuria, V. S. Bhatia, A. Rani, Pushpendra, S. M. Husain, C. T. Satyavathi, G. S. Chauhan, T. Mohapatra. Identification of SSR markers associated with seed coat permeability and electrolyte leaching in soybean. Physiology and Molecular Biology of Plants 14 (2008b) 173-177.
-  L. Rajjou, I. Debeaujon. Seed longevity: survival and maintenance of high germination ability of dry seeds. Crop Research Biology 331 (2008) 796-805.
-  L. Ho-Sun, J. Young-Ah, L. Young-Yi, L. Sok-Young, K. Yeon-Gyu. Comparison of seed viability among 42 species stored in a genebank. Korean Journal Crop Science 58(4) (2013) 432-438.
-  T. D. Hong, S. Linington, R. H. Ellis. Appendix 1. List of viability constants for use in the viability equation of Ellis and Roberts (1980a) and predicted seed storage longevity. In T. D. Hong, S. Linington, R. H. Ellis, (Eds) Compendium of information on seed storage behavior, vol. 2, I‒Z, Kew : The Royal Gardens, Kew, (1998) 881-882.
-  R. H. Ellis, T. D. Hong. Quantitative response of the longevity of seed of twelve crops to temperature and moisture in hermetic storage. Seed Science and Technology 35 (2007) 432-444.
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