Orexin receptor-1 expression in adult rodent neurogenic regions: evidence from the subgranular zone and the median eminence

• Authors: Antonello Pinna , Artur Pałasz

Title variants

PL

Ekspresja receptora oreksynowego-1 w regionach neurogenezy dorosłych gryzoni: strefie podziarnistej oraz wyniosłości pośrodkowej

• Languages of publication: EN PL

Abstracts

EN

INTRODUCTION: Orexin signaling plays a vital role in regulating autonomic and cognitive functions, including sleep-wake cycles, feeding, and memory. Orexin receptor-1 (OX1R), a key component of this system, may also influence adult neurogenesis. This study examined OX1R expression in both the classic (hippocampal) and non-classic (hypothalamic) neurogenic regions of the adult rodent brain. MATERIAL AND METHODS: Adult rodent brains were fixed, paraffin-embedded, and sectioned coronally. Immunohistochemistry and immunofluorescence were performed using antibodies against OX1R, DCX, and TUC-4, followed by fluorophore- or diaminobenzidine-based detection. Negative controls were included to ensure specificity. RESULTS: OX1R-positive cells were localized primarily to the subgranular zone (SGZ) of the dentate gyrus and β2 tanycytes of the median eminence showed uniform OX1R expression in both somata and vascular-directed processes. Morphological variation was observed between species, with diverse perikaryon shapes in mice and predominantly elongated, multipolar forms in rats. CONCLUSIONS: This study revealed, for the first time, region-specific OX1R expression in the SGZ in β2 tanycytes of the median eminence. These findings suggest a potential role for orexin signaling in adult neurogenesis.

PL

WSTĘP: Sygnalizacja oreksynergiczna odgrywa kluczową rolę w regulacji funkcji autonomicznych i poznawczych, w tym cyklu snu i czuwania oraz przyjmowania pokarmu. Receptor oreksyny-1 (orexin receptor-1 – OX1R), pierwszoplanowy element tego układu, może również wpływać na neurogenezę w mózgu dojrzałym. W przedstawionym badaniu zbadano ekspresję OX1R zarówno w klasycznych (hipokamp), jak i nieklasycznych (podwzgórze) regionach neurogennych mózgu dorosłych gryzoni (szczurów i myszy). MATERIAŁ I METODY: Mózgi dorosłych gryzoni utrwalono, zatopiono w parafinie i skrojono w płaszczyźnie poprzecznej. Badania immunohistochemiczne i fluorescencyjne wykonano z wykorzystaniem pierwszorzędowych przeciwciał selektywnych względem OX1R, DCX i TUC-4, a następnie przeciwciał drugorzędowych sprzężonych z fluorochromami lub w reakcji diaminobenzydyny. W celu zapewnienia swoistości uwzględniono kontrole negatywne. WYNIKI: Komórki OX1R-pozytywne zlokalizowano głównie w strefie podziarnistej (subgranular zone – SGZ) zakrętu zębatego, β2-tanycyty wyniosłości pośrodkowej wykazywały jednorodną ekspresję OX1R zarówno w ich ciałach komórkowych, jak i w wypustkach okołonaczyniowych. Zaobserwowano istotne zróżnicowanie morfologiczne neuronów OX1R-pozytywnych między badanymi gatunkami gryzoni – u myszy dominowały perykariony różnokształtne, natomiast u szczurów komórki wydłużone i wielobiegunowe. WNIOSKI: Badanie to po raz pierwszy ujawniło specyficzną dla regionu ekspresję OX1R w SGZ hipokampa oraz w β2-tanycytach wyniosłości pośrodkowej podwzgórza. Wyniki sugerują potencjalną rolę sygnalizacji oreksynowej w regulacji neurogenezy w mózgu dojrzałym.

Keywords

EN

adult neurogenesis; hipokamp; hippocampus; neurogeneza w mózgu dojrzałym; oreksyna; orexin; tanycytes; tanycyty

Discipline

• MEDICINE: MEDICINE

• Publisher: Śląski Uniwersytet Medyczny w Katowicach
• Journal: Annales Academiae Medicae Silesiensis
• Year: 2025
• Volume: 79
• Pages: 308–315

Contributors

author

Antonello Pinna

• Department of Histology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland

author

Artur Pałasz

• Department of Histology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland

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• Document Type: article