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2018 | 23 | 97 - 102

Article title

DETERMINATION OF THE HYDROLYTIC ACTIVITY OF WHOLE SALIVA USING CHITOSAN ASCORBATE AS A SUBSTRATE

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EN

Abstracts

EN
The aim of this work was to evaluate of hydrolytic activity of whole saliva using chitosan ascorbate as a substrate. In this aim, the concentrations of N-acetyl-D-glucosamine were determined in saliva before addition of chitosan ascorbate, directly after addition and during incubation with chitosan ascorbate by 20 hrs. In this in vitro study were used sterile chitosan ascorbate in the form of powder. Chitosan was obtained from krill chitin. The ratio of ascorbic acid to chitosan was 1:1. The unstimulated whole saliva showed the hydrolytic activity in the presence of the chitosan ascorbate as a substrate.

Contributors

  • Department of Conservative Dentistry, Medical University of Gdańsk
  • Department of Conservative Dentistry, Medical University of Gdańsk
  • Department of Conservative Dentistry, Medical University of Gdańsk

References

  • [1] Schenkels LCP, Veerman ECI, Nieuw Amerongen AV; (1995) Biochemical composition of human saliva in relation to other mucosal fluids. Crit Rev Oral Biol Med. 6, 2, 161-175.
  • [2] Siqueira WL, Dawes C; (2011) The salivary proteome: Challenges and perspectives. Proteomics Clin Appl. 5, 575-579. DOI 10.1002/prca.201100046.x
  • [3] Bridges J, Smythe J, Reddrick R; (2017) Impact of salivary enzyme activity on the oral perception of starch containing foods. J Texture Stud. 48, 288-293. DOI:10.1111/jtxs.12252.x
  • [4] Neyraud E, Palicki O, Schwartz C, Nicklaus S, Feron G; (2012) Variability of human saliva composition: Possible relationships with fat perception and liking. Arch Oral Biol. 57, 556-566.
  • [5] Barwińska-Płużyńska J, Kochańska B, Ochocińska J; (2014) Stimulated salivary flow rate and the level of selected salivary proteins in elderly people population. J Stomat. 67, 6, 762-779.
  • [6] Gajda E, Bugla-Płoskońska G; (2014) Lysozyme – occurrence in nature, biological properties and possible applications. Postepy Hig Med Dosw (online). 68, 1501-1515. e-ISSN 1732-2693.
  • [7] Aiba S; (1994) Preparation of N-acetylchitooligosaccharides from enzymic hydrolysates of chitosans. In: Karnicki ZS, Wojtasz-Pajak A, Brzeski MM, Bykowski P (eds) Chitin World. Monograph. Wirtschaftsverlag NW, Bremerhaven, 108-111.
  • [8] El-Sayed ST, Nagwa I. Omar NI, El-Sayed M, Shousha WG; (2017) Evaluation antioxidant and cytotoxic activities of novel chitooligosaccharides prepared from chitosan via enzymatic hydrolysis and ultrafiltration. J Applied Pharm Sci. 7, 11, 050-055. http://www.japsonline.com, DOI:10.7324/JAPS.2017.71107.x
  • [9] Rokhati N, Susanto H, Haryani K, Pramudono B; (2017) Enhanced Enzymatic Hydrolysis of Chitosan by Surfactant Addition. Period Polytech Chem Eng. https://doi.org/10.3311/PPch.11142.x
  • [10] Ahsan SM, Thomas T, Reddy KK, Sooraparaju SG, Asthana A, Bhatnagar I; (2017) Chitosan as biomaterial in drug delivery and tissue engineering. Int J Biol Macromol. http://dx.doi.org/10.1016/j.ijbiomac.2017.08.140.x
  • [11] Islam S, Rahman Bhuiyan MA, Islam MN; (2017) Chitin and Chitosan: Structure, Properties and Applications in Biomedical Engineering. J Polym Environ. 25:854–866. DOI 10.1007/s10924-016-0865-5.x
  • [12] Kochańska B, Śramkiewicz J; (2000) Evaluation of chitosan ascorbate application as a multifunctional dressing during dental operation within the region of dental cervix. In: Muzzarelli RAA (ed) Chitosan per os: from dietary supplement to drug carrier. Grottammare, 257-264.
  • [13] Kochańska B, Witek E, Śramkiewicz J; (1994) Haemostatic properties of chitosan and its application in stomatology. In: Karnicki ZS, Wojtasz-Pajak A, Brzeski MM, Bykowski P (eds) Chitin World. Monograph. Wirtschaftsverlag NW, Bremerhaven, 520-529.
  • [14] Kochańska B, Kędzia A, Gębska A; (2016) Sensitivity to chitosan ascorbate microaerophilic bacteria isolated from infections of oral cavity. PCACD 21, 109-113.
  • [15] Reissig JL, Strominger JL, Leloir LF; (1955) A modified colorimetric method for the estimation of N-acetyloamino sugars. J Biol Chem. 217, 959-966.
  • [16] Kochańska B; (1997) Biodegradation of chitosan in saliva: In vitro studies. In: Struszczyk H (ed) Progress on Chemistry and application of chitin and its derivatives. Monograph. PTCH, Łódź, III, 103-108.
  • [17] Sashiwa H, Saito K, Saimoto H, Minami S, Okamoto Y, Matsuhashi A. Shigemasa Y; (1993) Enzymatic degradation of chitin and chitosan. In: Muzzarelli RAA (ed) Chitin Enzymology. Monograph. Eur Chitin Soc. Ancona, 177-186.
  • [18] Zhang J, Xia W, Liu P, Cheng Q, Tahirou T, Gu W, Li B; (2010) Chitosan Modification and Pharmaceutical/Biomedical Applications. Mar. Drugs. 8, 1962-1987; DOI:10.3390/md8071962.
  • [19] Li J, Du Y, Liang H; (2007) Influence of molecular parameters on the degradation of chitosan by a commercial enzyme. Polymer Degradation and Stability. 92, 3, 515-524. https://doi.org/10.1016/j.polymdegradstab.2006.04.028.

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article

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bwmeta1.element.psjd-4332652f-1421-4ca5-a572-03433933e823
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