Preferences help
enabled [disable] Abstract
Number of results
2019 | 115 | 15-26
Article title

Anti-MRSA Activity of Padina tetrastromatica, Padina gymnospora from Gulf of Mannar Biosphere

Title variants
Languages of publication
Nowadays coming out of bacterial resistance poses a significant clinical problem. Hence, the aim of this study was to explain the current susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA).As well as to find out antimicrobial characteristics in the different organic solvents with increasing polarity viz., hexane, chloroform, ethyl acetate, and methanol extracts of Padina tetrastromatica, Padina gymnospora marine macro algae belonging to the family Phaeophyta were studied. Their crude extracts were tested against Staphylococcus aureus (MTCC 737 & 7443), and three clinical isolates of MRSA were tested and has been shown to exhibit antibacterial activity against methicillin methicillin-resistant Staphylococcus aureus (MRSA). The Minimum inhibitory concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) were determined. The ethyl acetate extracts of the seaweeds showed the presence of Photochemical, terpenoids, tannins, phenolic compounds and steroids strongly than the other solvent extracts. The highest activity was recorded in the ethyl acetate extract of Padina tetrastromatica than the other extract tested. The mean zone of inhibition produced by the extracts in disc diffusion assays against the tested bacterial strains ranged from 7.1 to 26.5 mm. The lowest MIC (62.5 µg/ml) and MBC (125 µg/ml) values were observed in the ethyl acetate extract of P. tetrastromatica against S. aureus (737 & 7443), MRSA1 and MRSA3. Further separation of active principle from the potential seaweed extract as a source of antibacterial compound useful for the control of Methicillin resistant S. aureus is under progress.
Physical description
  • Department of Botany, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India
  • Department of botany, St. Joseph University, Dimapur-797115, Nagaland, India
  • Department of Botany, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India
  • Department of Botany, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India
  • [1] Klevens RM, Morrison MA, Nadle J, Petit S, Gershman K, Ray S, Harrison LH, Lynfield R, Dumyati G, Townes JM, Craig AS, Zell ER, Fosheim GE, McDougal LK, Carey RB, Fridkin SK. Invasive methicillin-resistant Staphylococcus aureus infections in the United States. JAMA 298 (2007) 1763-1771.
  • [2] Lavanya R, Veerappan N Antibacterial Potential of Six Seaweeds Collected from Gulf of Mannar of the Southeast Coast of India. Advances in Biological Research 5 (1) (2011) 38-44.
  • [3] DH Tambekar, SB Dahikar. Antibacterial activity of some Indian ayurvedic preparations against enteric bacterial pathogens .Journal of advanced pharmaceutical technology & research 2 (1) (2011) 24–29.
  • [4] Lee DS, Isolation and characterization of anti-MRSA (methicillin-resistant Staphylococcus aureus) substances from marine organisms. Ph.D. Dissertation, Department of Microbiology, Pukyong National University, Busan. Chapter 1 (2009) 2-23.
  • [5] Kim, H.I., D. Lee, H.S. Lee, J. Ha, B. Ha, S. Kim and J. Lee, Antibacterial activity of Ulva lactuca againt methicillin-resistant Staphylococcus aureus (MRSA). Biotechnology and bioprocess Engineering (2007) 579-582.
  • [6] Finland, M. Emergence of antibiotic resistance in hospitals, 1935-1975. Reviews of infectious diseases 1(1) (1979) 4.
  • [7] Brumfitt, W. & J. M. T. Hamilton-Miller: The worldwide problem of methicillin-resistant Staphylococcus aureus. Drugs Under Experimental and Clinical Research 16(5) (1990) 205-214.
  • [8] [Isnansetyo, A. and Y. Kamei MC21-A, a bactericidal antibiotic produced by a new marine bacterim, Pseudoalteromonas phenolica sp. nov. O-BC30T, against methicillin-resistant Staphylococcus aureus. Antimicrobial agents and chemotheraphy 47 (2003) 2-488.
  • [9] Eom SH, Kim DH, Lee SH, Yoon NY, Kim JH, Kim TH, Chung YH, Kim SB, Kim YM, Kim HW, Lee MS, Kim YM. In vitro antibacterial activity and synergistic antibiotic effects of phlorotannins isolated from Eisenia bicyclis against methicillin resistant Staphylococcus aureus. Phototherapy Research 27 (2013) 1260–1264.
  • [10] Liu C, Graber CJ, Karr M. A population-based study of the incidence and molecular epidemiology of methicillin-resistant Staphylococcus aureus disease in San Francisco, 2004–2005. Clinical Infectious Diseases 46 (2008) 1637-1646.
  • [11] Eom SH, Park JH, Yu DU, Choi JI, Choi JD, Lee MS, Kim YM Antimicrobial activity of brown alga Eisenia bicyclis against methicillin resistant Staphylococcus aureus. Fisheries and Aquatic Sciences 14 (2011) 251-256.
  • [12] Smit, A.J.Medicinal and pharmaceutical uses of seaweed natural products. A review. Journal of Applied Phycology 16 (2004) 245-262.
  • [13] Cabrita, M., Vale, C., Rauter, A. Halogenated compounds from marine algae. Marne Drugs 8 (2010) 2301-2317.
  • [14] Bennamara, A., A. Abourriche, M. Berrada, M. Charrouf, N. Chaib, M. Boudouma, and F.X. Garneau. Methoxybifurcarenone: an antifungal and antibacterial meroditerpenoid from the brown alga Cystoseira tamariscifolia. Phytochemistry 52(1) (1999) 37-40.
  • [15] Nagayama, K., Y. Iwamura, T. Shibata, I. Hirayama, and T. Nakamura. Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. Journal of Antimicrobial Chemotherapy 50 (2002) 889-893.
  • [16] Nunnery JK, Mevers E, Gerwick WH. Biologically active secondary metabolites from marine cyanobacteria. Current Opinion in Biotechnology 21 (2010) 787-793.
  • [17] Mayer AM, Rodriguez AD, Berlinck RG, Fusetani N. Marine pharmacology in 2007-8: marine compounds with antibacterial, anticoagulant, antifungal, anti-inflammatory, antimalarial, antiprotozoal, antituberculosis and antiviral activities; affecting the immune and nervous system and other miscellaneous mechanism of action. Comparative Biochemistry and Physiology 153 (2011) 191-222.
  • [18] Cabrita MT, Vale C, Router AP. Halogenated compounds from marine algae. Marain Drugs 8 (2010) 2301-2317.
  • [19] Harborne, I. B. Phytochemicals methods: A guide to modern techniques of plant analysis. 2nd edn, Chapman and Hall, New York, (1973) 88-185.
  • [20] Trease, G.E. and Evans, W.C.Textbook of pharmacognosy. 12th Edition, Tindall and Co., London, (1983) 343-383.
  • [21] CLSI (Clinical Laboratory Standard Institute) (formerly NCCLS), Performance standards for antimicrobial disk susceptibility tests: Wayne, PA, USA. 100-22: 32-3.
  • [22] Colle J.G., A.G. Frasher, B.P. Marmiom, A. Simmon Makie and McCartney’s Practical Medical Microbiology, 14th edn., Churchill Livingstone, (1996) 254-256: 796-8.
  • [23] Bauer A.W., W.M.M. Kirby, J.C. Scherris, M. Turck. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45 (1966) 493-496.
  • [24] Sarker S. D., L. Nahar, Y. Kumarasamy Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 42 (2007) 321-324.
  • [25] Oumaskour, K., N. Boujaber, S. Etahiri and Assobhei, O. Screening of antibacterial and antifungal activities in green and brown algae from the coast of Sidi Bouzid (El Jadida, Morocco). African Journal of Biotechnology 11 (104) (2012) 16831-16837.
  • [26] Nagayama, K., Y. Iwamura, T. Shibata, I. Hirayama, and Nakamura, T. Bactericidal activity phlorotannins from the brown alga Ecklonia kurome. Journal of Antimicrobial Chemotheraphy 50 (2002) 889-893.
  • [27] Thillairajasekar K., V. Duraipandiyan, P. Perumal, S. Ignacimuthu Antimicrobial activity of Trichodesmium erythraeum (Ehr) (microalaga) from South East Coast of Tamil Nadu, India. International Journal of Integrative Biology 5 (2009) 167-170.
  • [28] Salem W. M., H. Galal, F. Nasr El-deen Screening for antibacterial activities in some marine algae from the red sea (Hurghada, Egypt). African Journal of Microbiology Research 5(15) (2011) 2160-2167.
  • [29] Lavanya R. and N. Veerappan Antibacterial Potential of Six Seaweeds Collected from Gulf of Mannar of Southeast Coast of India. Advances in Biological Research 5 (1) (2011) 38-44.
  • [30] Kim, I.H., S.H. Lee, J.M. Ha, B.J. Ha, S.K. Kim and J.H. Lee, Antibacterial activity of Ulva lactuta against methicillin- resistant Staphylococcus aureus (MRSA). Biotechnology and Bioprocess Engineering 12 (2007) 579-582.
  • [31] Lee, D.S., M.S. Kang, H.J. Hwang, S.H. Eom, J.Y. Yang, M.S. Lee, W.J. Lee, Y.J. Jeon, J.S. Choi and Y.M. Kim, Synergistic effect between deckle from Ecklonia stolonifera and β-lactams against methicillin-resistant Staphylococcus aureus. Biotechnology Bioprocess Engineering 13 (2008) 758-764.
  • [32] Chandrasekaran, M., V. Venkatesalu and G. Adaikala Raj, 2014a. Antibacterial activity of selected marine macro algae against Vancomycin resistant Enterococcus faecalis. Journal of Coastal Life Medicine. 2(12) (2014) 940-946.
  • [33] Chandrasekaran, M., V. Venkatesalu and G. Adaikala Raj, 2014b. Anti-MRSA activity of brown and red algae from Gulf of Mannar coast, South India. Journal of Life Sciences and Technology. 7(4) (2014) 22-31; 0974-5335.
  • [34] Shanmughapriya, S., A. Manilal, S. Sujith, J. Selvin, G. Seghal kiran and K. Nataraja seenivasan, Antimicrobial activity of seaweeds extracts against multiresistant pathogens. Annals of Microbiology 58 (3) (2008) 535-541.
  • [35] Usmanghani, K. and M. Shameel, Studies on the antimicrobial activity of certain seaweeds from Karachi coast. In: R. Ahmad and A.S. Pietro (Eds.), Prospects for Biosaline Research, Proc. US-Pak. Biosal. Res. Works, Karachi, pp. 519-526.
  • [36] Reguant C., A. Bordons Arola, L N. Roze Influence of phenolic compounds on the physiology of Oenococcus oeni. Journal of Applied Microbiology 88 (2000) 1065–1071.
  • [37] Zubia M., C. Payri, E. Deslandes Alginate, mannitol, phenolic compounds and biological activities of two range-extending brown algae, Sargassum mangarevense and Turbinaria ornata (Phaeophyta: Fucales), from Tahiti (French Polynesia). Journal of Applied Phycology 20(6) (2008) 1033-1043.
  • [38] Ivanchina N.V., A. A. Kicha, V.A. Stonik Steroid glycosides from marine organisms. Steroids 76 (2011) 425-454.
  • [39] Scalbert A. Antimicrobial properties of tannins. Photochemistry, Phyfochemisrry, 30 (12) (1991) 3875-3883.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.