PL EN


Preferences help
enabled [disable] Abstract
Number of results
2019 | 125 | 245-251
Article title

The effect of NAA and BAP on the multiplication of chamomile (Matricaria chamomilla L.) callus tissue in vitro

Content
Title variants
Languages of publication
EN
Abstracts
EN
The aim of the study was to determine the effect of the additive on NAA and BAP on the formation of callus tissue on leaf chamomile explants, cultivar, and to determine the optimal content of said growth regulators for the initiation of callus tissue in vitro. During the experiment fragments of chamomile from the collection of the Department of Genetics, Breeding and Biotechnology of Plant were laid on a medium with mineral composition according to Murashige and Skoog - MS (1962) enriched with auxin NAA in concentrations of 3 and 5 mg·dm-3 and cytokinin BAP in a concentration of 1, 2 , 5, 7 mg·dm-3. Based on the conducted experiment it was found that the initiation of callus chamomile tissue is possible in the case of using leaf explants lined with MS in the combination of auxin NAA in concentration 3 or 5 mg·dm-3 and BAP from 1 to 7 mg·dm-3. The propagation of chamomile callus tissue should be carried out on MS proliferating medium enriched with NAA at a rate of 3 mg·dm-3 and BAP in an amount of 7 mg·dm-3. Explants laid on a medium with this composition develop a large mass of callus tissue.
Year
Volume
125
Pages
245-251
Physical description
Contributors
author
  • Department of Genetics, Plant Breeding and Biotechnology, Faculty of Environmental Sciences and Agriculture, West Pomeranian University of Technology in Szczecin, 17 Słowackiego Str., 71-434 Szczecin, Poland
author
  • Department of Genetics, Plant Breeding and Biotechnology, Faculty of Environmental Sciences and Agriculture, West Pomeranian University of Technology in Szczecin, 17 Słowackiego Str., 71-434 Szczecin, Poland
References
  • [1] T. Furmanek, W. Banaś, A. Banaś,. Embryogenic callus formation by cotyledon and leaf explants of (Crambe sbyssinica) seedlings. Journal of Biotechnology, Computational Biology and Bionanotechnology Vol. 92(2) (2011) 209-213
  • [2] Gatz, T. Kowalski, Trachery element differentiation and morphogenetic changes in callus (Capsicium annuum L.). Acta Scientiarum Polonorum, Hortorum Cultus 10(1) (2011) 131-146
  • [3] E. Hanus-Frajerska, K. Ciarkowska, The effecy of (Nicotiana tabacum L.) extracts on cultures of tobacco callus. Acta Scientiarum Polonorum, Hortorum Cultus Vol. 11(4) (2012) 59-66
  • [4] L. KeShun, Soybeans chemistry, technology, and utilization. Springer-Science + Business Media Dordrecht (1997) 4-22
  • [5] S. Bhatia. T. Bera, R. Dahiya. T. Bera. Plant Biotechnology in Pharmaeutical Sciences. Academic Press is an imprint of Elsevier (2015) 32-89
  • [6] S. Kintzios. A. Michaelakis, Induction of somatic embryogenesis and in vitro flowering from inflorescences of chamomile (Chamomilla recutita L.). Plant Cell Report Vol. 18 (1999) 684-690
  • [7] O. Singh. Z. Khanam. N. Neelam. S. Manoj – Kumar, Chamomile (Matricaria chamomilla L.): An overvie. Pharmaogn Review Vol. 5 (2011) 82-95
  • [8] M. Krupa-Małkiewicz. K. Zieliński, The influence of plant growth regulators (2,4-D, BAP, GA3) on the shoots propagation and callus tissue induction of sweet basil (Ocimum basilicum L.). Folia Pomeranae Universitatis Technologiae Stetinensis. Agricultura, Alimentaria Vol. 26 (2013) 33-38
  • [9] S. Kumar. J. Kumar-Kamawar, Plant regeneration from cell suspension in (Gerbera jamesonii Bolus). Journal of Fruit and Ornamental Plant Research Vol. 15 (2007) 157-166
  • [10] P. Gardiner. Chamomile (matricaria reutita, Anthemis nobilis). Longwood Herbal Task Force. (1999) 1-21
  • [11] T. Murashige. F. Skoog, A revised medium for rapid growth and bioassays with Tobacco tissue culture. Physiologia Plantarum Vol. 15 (1963) 473-497
  • [12] M. Rafieohossaini. H. Sodaeizadeh. A. Adams. N. Kimpe, P. Damme, Effects of planting date and seedling age on agro-morphological characteristics, essential oil content and composition of German chamomile (Matricaria chamomilla L.) grown in Belgium. Industrial Crops and Products Vol. 31 (2010) 145-152
  • [13] D. Rzepka-Plevnes. D. Kulpa. M. Grabiec. K. Kowalczys. J. Kurek, The effect of growth regulators and culture conditions on the callus induction in tomato Lycopersicon sp. Acta Scientiarum Polonorum. Hortorum Cultures Vol. 5(2) (2006) 23-34
Document Type
short_communication
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.psjd-393a56ad-88f0-40a6-ba60-5a9193c6f693
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.