THE ROLE OF CHITOSAN IN AKT KINASE REGULATION ACTIVITY

• Authors: Jan Ignacak , Maria Wiśniewska-Wrona , Joanna Dulińska-Litewka , Iwona Pałka , Magdalena Kucharska , Jan Kazimierski
• Languages of publication: EN

Abstracts

EN

A decrease in migration of tumor cells incubated with the investigated chitosan preparations was correlated with a decreased activity of MMP-2 and MMP-9 metalloproteinases, what significantly affected inhibition of tumor cell proliferation. In the investigations of the effects of various chitosan preparations on expression of PCNA, Akt and β-catenin in the normal human 184A1 cells and in breast carcinoma MCF7 cells evaluated at the protein level, significant differences in inhibition of expression of selected genes were noted in the tumor cells. Similarly as in the case of human cells, in mouse cells, the differences in expression of the investigated genes involved solely the Ehrlich carcinoma cells. In the presence of the investigated chitosan preparations, there was observed inhibition of expression of the N-cadherin, β-catenin, Akt and PCNA genes. In case of p21 protein, its level increased, similarly as in the human breast carcinoma cells, what may also be related to phosphorylation of the protein, its capture by the cytosol and prolonging its half-life as compared to the non-phosphorylated form. In case of the normal human 181A1 cells and mouse CRL 1636 cells, no significant alterations were noted in expression of the investigated genes in presence of the employed chitosan preparations.

Keywords

EN

Akt kinase (PKB); M2 pyruvate kinase; cells migration; metalloproteinases

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2016
• Volume: 21
• Pages: 73-82

Contributors

author

Jan Ignacak

• Chair of Medical Biochemistry, Medical College, Faculty of Medicine, Jagiellonian University Kopernika 7, 31-034 Kraków,

author

Maria Wiśniewska-Wrona

• Institute of Biopolymers and Chemical Fibres, M. Skłodowskiej-Curie 19-27, 90-570 Łódź,

author

Joanna Dulińska-Litewka

• Chair of Medical Biochemistry, Medical College, Faculty of Medicine, Jagiellonian University Kopernika 7, 31-034 Kraków,

author

Iwona Pałka

• Chair of Medical Biochemistry, Medical College, Faculty of Medicine, Jagiellonian University Kopernika 7, 31-034 Kraków,

author

Magdalena Kucharska

• Institute of Biopolymers and Chemical Fibres, M. Skłodowskiej-Curie 19-27, 90-570 Łódź,

author

Jan Kazimierski

• Poli-Farm Company o.o. Łyszkowicka 59, 99-400 Łowicz,

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• Document Type: article