Isolation and characterisation of alpha-amylase producing yeast from different fermented foods and dairy products

• Authors: F. T. Afolabi , O. O. Adetayo
• Languages of publication: EN

Abstracts

EN

The use of acids in the hydrolysis of starch for industrial and domestic purpose have become relatively unstable, expensive, difficult to control and even harmful to users over the years. This study aimed at isolating and characterizing alpha -amylase producing yeasts and investigate the effects of different cultural and physico-chemical parameters on the production of alpha- amylase. A total of forty- three (43) yeast strains were isolated from different fermented foods such as ogi, wara, palm wine and kunu. The isolates (OG9 and WR4) had the highest activity and were identified as: Candida tropicalis strain IFM 63517 and Candida tropicalis strain CMC 1836 respectively. Fermentation parameters such as incubation period, pH, temperature, effect of surfactants, carbon and nitrogen source were optimized using submerged fermentation. The optimum pH, temperature and incubation period for enzyme production were: 5.0, 30 °C and 48 hours respectively. Of the carbon sources, soluble starch at 1% concentration was observed to be the best carbon source for enzyme production using the two yeasts. Yeast extract at 1% concentration was ideal nitrogen source for the yeasts. Surfactants Tween-80 was most effective for enhancement of alpha amylase production. Cacl2 supported the highest alpha amylase activity for both strains. This study revealed that favourable fermentation conditions and the selection of suitable growth parameters played key roles in the production of alpha amylase by Candida tropicalis. Soluble starch is a good substrate for alpha amylase production. Alpha- amylase producing yeast strains with increasing enzyme activities at high temperature are promising candidates for industrial application.

Keywords

EN

Alpha amylase; Candida tropicalis; Yeast; fermentation; soluble starch

Discipline

• MICROBIOLOGY: MICROBIOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2022
• Volume: 44
• Pages: 89-110

Contributors

author

F. T. Afolabi

• Department of Microbiology, Faculty of Science, University of Ibadan, Ibadan, Nigeria

author

O. O. Adetayo

• Department of Microbiology, Faculty of Science, University of Ibadan, Ibadan, Nigeria

References

• [1] Arnesen S, Eriksen S.H., Olsen J, and Jensen B (1998). Increased production of alpha amylase from Thermomyces lanuginosus by the addition of Tween-80. Enzyme Microbial Technology. Vol. 23, No. 3-4 pp. 249–252
• [2] Arnold K., Kersti K, and Marko L (2011). Extraction of genomic DNA from yeasts for PCR- based applications. Biotechniques 50(5): 325-328 doi: 10.2144/000113672
• [3] Balkan B. and Ertan F. (2007). Production of alpha amylase from Penicillium Chrysogenum. Food Technology and Biotechnology 45: 439-442
• [4] Banwo K., Omotade R.. Sanni A.I. and Alakeji T.P (2015. Functional properties of yeasts isolated from some Nigerian traditional fermented foods. Journal of Microbiology, Biotechnology and Food Sciences. 4(5): 437-441
• [5] Beyer S. (2011). Enzymes for industrial applications: Global markets. Glob Indus Anal., Inc.
• [6] Burhan A., Nisa U., Gokhan C., Omer C., Ashabil A. and Osman G. (2003). Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6. Process Biochemistry 38: 1397-403
• [7] Chi Z., Liu G., Wang F. and Zhang L. (2009). Saccharomycopsis fibuligera and its applications in biotechnology. Biotechnology Advances 27: 423-431
• [8] Crabb W. and Shetty J. (1999). Commodity scale production of sugars from starches. Current Opinion in Microbiology, Vol. 2, pp. 252–256
• [9] Fabiana C.P ., Eleni G. and Roberto S. (2008). Isolation, characterization and optimization of cultural conditions for amylase production from fungi. Brazilian Journal of Microbiology 39: 108-114
• [10] Fossi B.T., Tavea F., Jiwoua C. and Ndjouenke R. (2009). Screening and phenotypic characterization of thermostable amylases producing yeasts and bacteria strains from some Cameroonian soils. African Journal of Microbiological Research. 3(9): 504-514
• [11] Gashaw Mamo and Amare Gessesse (1999b). Effect of cultivation conditions on the growth and α-amylase production by a thermophilic Bacillus sp. Letter in Applied Microbiology 29: 61-65
• [12] Gupta R., Giras P., Mohapatra H., Gaswami Y.K. and Chauhan B. (2003). Microbialα-amylase: a biotechnological perspective. Process Biochemistry 38(11): 1599-1616
• [13] Haq I., Ashraf H., Qadeer M.A. and Iqbal J. (2003). Production of alpha-amylase by Bacillus licheniformis using an economical medium. Bioresource Technology 87: 57-61
• [14] Hoa B.T. and Hung P.V.(2013). Optimization of nutritional composition and fermentation conditions for cellulase and pectinase production by Aspergillus oryzae using response surface methodology. International Food Research Journal 20: 3269-3274
• [15] Holker U., Hofer M. and Lenz J. (2004). Biotechnological advantages of laboratory-scale solid state fermentation with fungi. Applied Microbiology and Biotechnology 64: 175-186
• [16] Hostinova E. (2002). Amylolytic enzyme produced by the yeast Saccharomycopsis fibuligera. Biological Bratislava 57(11): 247-251
• [17] Kirk O., Borchert T.V. and Fuglsaang C. (2002). Industrial enzyme applications. Current Opinion in Biotechnology 13: 345-351
• [18] Kumar A. and Duhan J.S. (2011). Production and Characterization of Amylase Enzyme Isolated from Aspergillus niger MTCC-104 Employing Solid State Fermentation. International Journal of Pharmaceutical and Bio- Sciences 2(3): 250-258
• [19] Lowry O.H., Rosebrough N.J. and Farr A.L. (1951). Protein Measurement with the Folin Phenol Reagent. Journal of Biological Chemistry 193: 265-275
• [20] Maarel van der, Van der Veen B, Uitdehaag Leemhuis H. and Dijkhuizen L. (2005). Properties and applications of starch converting enzymes of the alpha-amylase family. Journal of Biotechnology 94: 137–155
• [21] McMohan E.M., Ketty C.T. and Forgarty W.M. (1999). High maltose producing amylolytic system of a Streptomyces sp. Biotechnology Letters 21(1): 23-26
• [22] Moreira F.G, Lima F.A, Pedrinho S.R.F, Lenartoviez V, Souza C.G.M, and Peralto R.M (1999). Production of amylases by Aspergillus tamari. Revolution Microbiology 30(2): 157-162
• [23] Pandey A., Nigam P., Soccol C., Soccol V., Singh D. and Mohan R. (2000). Advances in microbial amylases. Biotechnology and Applied Biochemistry Vol. 31, No. 2, pp. 135–152
• [24] Pederson H. and Neilson J. (2000). The influence of nitrogen sources on alpha amylases productivity of Aspergillus oryzae in continuous cultures. Applied Microbiology and Biotechnology 53: 278-281
• [25] Prabhakaran D. and Hewitt C.J. (2009). The production of amylase by Bacillus sp in a complex and a totally defined synthetic culture medium. Journal of Industrial Microbiology 17: 96-99
• [26] Rene D.M. and Hubert (1985). Purification and characterization of extracellular amylolytic enzymes from the yeast Filobasidium capsuligenum. Applied Environmental Microbiology 50(6): 1474-1482
• [27] Rick W. and Stegbauer H.P. (1974). α-amylase measurement of reducing groups. In (Ed. Bergmeyer HU). Methods of Enzymatic Analysis; pp. 55-56, Academic Press, New York.
• [28] Souza P.M. and Magalhães P.O. (2010). Application of microbialα-amylase in industry from thermophilic Bacillus sp. Brazilian Journal of Microbiology 33: 57-61
• [29] Srivastava R.A.K. and Baruah J.N. (1986). Culture conditions for production of thermostable amylase by Bacillus stearothermophilus. Applied and Environmental Microbiology 52: 179-184

• Document Type: article