Response of Soil Microbial Isolates to the Toxicity of Green Surfactant

• Authors: Doris I. Iroegbu , Ifechukwu E. Adieze , Christopher E. Nwanyanwu , Obioma K. Mejeha , Barinuale Sinee
• Languages of publication: EN

Abstracts

EN

In this study, microorganisms were obtained from hydrocarbon-polluted and unpolluted soil samples using Nutrient agar and Bushnell Hass agar for the enumeration of heterotrophic bacteria and hydrocarbon-utilizing bacteria respectively.The response of hydrocarbons utilizing soil bacterial isolates to the toxicity of green surfactant was assayed. The hydrocarbons utilizing bacterial isolates in soil extract broth were exposed to different concentrations; (1, 2, 3, 4, and 5) % w/v of green surfactants, and their responses (cell growth and viability) were time-dependent and monitored using spectrophotometer and total viable count. These hydrocarbon-utilizing bacteria isolates were two different species of Micrococcus (C and D), Bacillus sp. (F2) and Pseudomonas sp. (A2). They showed good response to high concentrations (4 and 5%) of the green surfactant in soil extract broth. This could be a result of a component of the green surfactant such as phosphorus which is an essential nutrient for microbial growth. Hence these tolerated green surfactant concentrations could be recommended to be used as surface active agents to enhance the bioavailability of hydrocarbon to microorganisms for bioremediation practices of hydrocarbon-contaminated sites.

Keywords

EN

Concentration; Green surfactant; Hydrocarbon; Responses; Soil isolates

Discipline

• MICROBIOLOGY: MICROBIOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2024
• Volume: 52
• Pages: 12-22

Contributors

author

Doris I. Iroegbu

• Department of Microbiology, Federal University of Technology, Owerri, Nigeria

author

Ifechukwu E. Adieze

• Department of Microbiology, Federal University of Technology, Owerri, Nigeria

author

Christopher E. Nwanyanwu

• Department of Microbiology, Federal University of Technology, Owerri, Nigeria

author

Obioma K. Mejeha

• Department of Microbiology, Federal University of Technology, Owerri, Nigeria

author

Barinuale Sinee

• Department of Microbiology, Federal University of Technology, Owerri, Nigeria

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• Document Type: article