Porównanie działania klozapiny i haloperidolu na grupy tiolowe osocza osób zdrowych

• Authors: Anna Dietrich-Muszalska , Anna Kwiatkowska , Justyna Kopka

Title variants

EN

The comparison of clozapine and haloperidol effects on plasma thiol groups in healthy subjects

• Languages of publication: EN

Abstracts

EN

Differentiated effects of antipsychotics: haloperidol and clozapine on oxidative stress biomarkers were analysed. It has not been determined yet in what way clozapine and haloperidol affect human plasma thiol groups (-SH). The study was aimed at establishing of the effects of haloperidol and clozapine, in doses recommended for treatment of acute episode of schizophrenia, on free thiols in human plasma under in vitro conditions. Material and methods: Blood for the study was collected from 10 healthy male volunteers (aged 24-26 years) for ACD solution. Active substance of the drugs was dissolved in 0.01% dimethyl sulfoxide to the final concentrations (haloperidol 4 ng/ml and 20 ng/ml; clozapine 350 ng/ml and 420 ng/ml) and incubated with plasma for 24 hours at 37°C. Control samples were performed for each experiment (without the drug). The free thiols level was measured using the Ellman method (acc. to Rice-Evans, 1991). The results were analysed using the paired Student t-test (StatSoft Inc., Statistica v. 6.0). Results: After 24 hours’ incubation with plasma, haloperidol in concentrations 4 ng/ml and20 ng/ml and clozapine in concentrations 350 ng/ml and 420 ng/ml caused statistically insignificant, as compared to control samples (without the drug), modifications in the level of free thiols (p>0.05). Conclusions: Clozapine and haloperidol in concentrations corresponding to doses recommended for treatment of acute episode of schizophrenia, do not induce significant changes in the concentration of free thiols in plasma.

PL

Analizowano zróżnicowane działanie leków przeciwpsychotycznych: haloperidolu i klozapiny na biomarkery stresu oksydacyjnego. Dotychczas nie rozstrzygnięto, w jaki sposób klozapina i haloperidol oddziałują na grupy tiolowe (-SH) osocza ludzkiego. Celem badania było ustalenie wpływu haloperidolu i klozapiny w dawkach rekomendowanych do leczenia ostrego epizodu schizofrenii na wolne tiole w ludzkim osoczu w modelu in vitro. Materiał i metody: Krew do badań pobrano od 10 zdrowych ochotników płci męskiej (w wieku 24-26 lat) na roztwór ACD. Substancję aktywną leków rozpuszczono w 0,01% dimetylosulfotlenku do stężeń końcowych (haloperidol 4 ng/ml i 20 ng/ml; klozapina 350 ng/ml i 420 ng/ml) i inkubowano z osoczem 24 godziny w temperaturze 37°C. Do każdego doświadczenia wykonano próby kontrolne (bez leku). Oznaczenia poziomu wolnych tioli wykonano metodą Ellmana (Rice-Evans, 1991). Do analizy wyników zastosowano sparowany test t-Studenta (StatSoft Inc., Statistica v. 6.0). Wyniki: Ustalono, że po 24-godzinnej inkubacji z osoczem haloperidol w stężeniu 4 ng/ml i 20 ng/ml oraz klozapina w stężeniu 350 ng/ml i 420 ng/ml spowodowały nieistotne statystycznie, w porównaniu z próbami kontrolnymi (bez leku), zmiany poziomu wolnych tioli (p>0,05). Wniosek: Klozapina i haloperidol w stężeniach odpowiadających dawkom rekomendowanym do leczenia ostrego epizodu schizofrenii nie powodują istotnych zmian stężenia wolnych tioli w osoczu.

Keywords

EN

clozapine; free thiols; haloperidol; klozapina; oxidative stress; schizofrenia; schizophrenia; stres oksydacyjny; wolne tiole

Discipline

• MEDICINE: MEDICINE

• Publisher: Medical Communications
• Journal: Psychiatria i Psychologia Kliniczna
• Year: 2012
• Volume: 12
• Issue: 4
• Pages: 255-264

Contributors

author

Anna Dietrich-Muszalska

• Pracownia Badań Biologicznych w Psychiatrii, I Katedra Psychiatrii, Uniwersytet Medyczny w Łodzi, Klinika Zaburzeń Afektywnych i Psychotycznych, Uniwersytet Medyczny w Łodzi

author

Anna Kwiatkowska

• Pracownia Badań Biologicznych w Psychiatrii, I Katedra Psychiatrii, Uniwersytet Medyczny w Łodzi

author

Justyna Kopka

• Pracownia Badań Biologicznych w Psychiatrii, I Katedra Psychiatrii, Uniwersytet Medyczny w Łodzi

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• Document Type: article