EXPRESS ANALYSIS OF CHITOSAN AND ITS DERIVATIVES BY GEL ELECTROPHORESIS

• Authors: Mariya Konovalova , Balzhima Ts. Shagdarova , Vitaliy Zubov , Elena Svirshchevskaya
• Languages of publication: EN

Abstracts

EN

Chitosan is one of the most promising polymers for biomedical applications due to its unique properties, such as its biocompatibility, low toxicity, biodegradation, and the presence of reactive amino and hydroxyl groups. Analysis of physicochemical properties of chitosan and its derivatives is a time-consuming process and requires expensive equipment and large amounts of the sample. This paper proposes a method for express analysis of the molecular weight (MW), the degree of deacetylation (DD), the substitution degree (SD), and the charge of chitosan and its derivatives using agarose gel electrophoresis under acidic and neutral conditions with Coomassie staining. Positively charged chitosan samples required acidic Tris Acetate-EDTA (TAE) buffer to move in the gel. The electrophoretic mobility of chitosan depended on MW, DD, SD, and the chitosan charge. Based on the dependences obtained by the proposed method, the MW and DD of commercial chitosan samples were determined. Express analysis of chitosan and its derivatives in agarose gel can be used to monitor the reactions of chitosan modification and to analyse samples with unknown characteristics.

Keywords

EN

agarose gel electrophoresis; chitosan; chitosan derivatives; degree of deacetylation; molecular weight

Discipline

• CHEMISTRY: CHEMISTRY

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2019
• Volume: 24
• Pages: 84 - 95

Contributors

author

Mariya Konovalova

• Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

author

Balzhima Ts. Shagdarova

• Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences

author

Vitaliy Zubov

• Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

author

Elena Svirshchevskaya

• Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

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• Document Type: article