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2019 | 23 | 13-23
Article title

Production of amylase by the intestinal microflora of cultured freshwater fishes (Oreochromis niloticus and Clarias gariepinus) rared localy in Calabar, south Nigeria

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This study focused on determining the amylase-producing ability of the intestinal microbes in cultured fresh water fishes – Oreochromis niloticus and Clarias gariepinus. The bacterial isolates were identified on the basis of standard cultural, morphological and biochemical characteristics. The amylase production ability of the bacterial isolates was determined using starch agar. The mean viable count of the intestinal microbes ranged from 1.2 × 105 CFU/ml to 7.1 × 105 CFU/ml for tilapias (Oreochromis niloticus) and from 2.0 × 104 CFU/ml to 8.9 × 104 CFU/ml for catfishes (Clarias gariepinus). Staphylococcus and Micrococcus were predominant for both tilapias and catfishes. Out of 24 isolates, 21 were amylase producers. These included the following bacteria genera: Bacillus, Micrococcus, and Staphylococcus. These results strongly suggest that intestinal microbes play a pivotal role in the digestion of starch in cultured freshwater fishes and should be explored for industrial amylase production.
Physical description
  • Department of Microbiology, Faculty of Biological Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria
  • Department of Microbiology, Faculty of Biological Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria
  • Department of Microbiology, Faculty of Biological Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria
  • [1] Abe, J., Bergman, F. W., Obata, K., and Hikuri, S. (1988). Production of Raw Starch Digesting Amylase by Aspergillus k-27. Applied Microbiology and Biotechnology, 27: 447-450.
  • [2] Austin, B. (2002). The Bacterial Microflora of Fish. The Scientific World Journal, 2: 558-572.
  • [3] Bailey, J. E., and Ollis, D. F. (1986). Starch Hydrolysis by Amylase. In Biochemical Engineering Fundamentals (2nd ed) (39-40). New York: McGraw-Hill.
  • [4] Barrow, G. I. and Feltham, R. K. A. (1993). In Cowan and Steel’s Manual for the Identification of Medical Bacteria (3rd ed) (pp 52-58, 87-89, 109-110). United Kingdom: Cambridge.
  • [5] Ben, A., Mexghani, M., Bejar, S. (1999). A Thermo-Stable Alpha-amylase Producing Maltohexose from a New Isolated Bacillus sp. US 100: Study of Activity and Molecular Cloning of the Corresponding Agent. Enzyme, Microbiology, Technology, 24: 548-549
  • [6] Berlin, S. (2004). Comparison of Digestive Alpha-amylase from Two Species of Spiders (Tegeneria atrica and Cupiennius Salei). Journal of Comparative Physiology, Biochemical, Systemic, and Environmental Physiology, 127 (4): 355-361.
  • [7] Cheesebrough, M. (2006). Preparation of Reagents and Culture Media. In District Laboratory Practice in Tropical Countries Part 2 (pp 394, 401). United Kingdom: Cambridge University Press.
  • [8] Cowan, S. T., Steel, K. J. (1993). Manual for the identification of medical bacteria. 2nd Ed. London: Cambridge University Press
  • [9] Erickson, H. M. (1992). Usage Recommendations for Alpha-amylase Maximizing Enzyme Activity while Minimizing Enzyme Artifact Binding Residues. The American Institute for Conservation, 11: 24 - 33.
  • [10] Gupta, R., Gigras, P., Mohapatra, H., Goswami, V. K., Chauhan, B. (2003). Microbial Alpha-amylases: A Biotechnological Perspective. Process Biochemistry, 38: 1599-1616.
  • [11] Izvekova, G. I., Izvekov, E. I., and Plotnikov, A. O. (2007). Symbiotic Microflora in Fishes of Different Ecological Groups. Ecology, 34 (6): 610-618.
  • [12] Naviner, M., Giraud, E., Le Bris, H., Armand, F., Mangion, C., and Ganiere, J. P. (2006). Seasonal Variability of Intestinal Microbiota in Rainbow Trout (Oncorhynchus mykiss), with a Particular Attention to Aeromonas spp. as Candidate Indicator of Antimicrobial Resistance. Revue de médecine vétérinaire 157(12): 599-604
  • [13] Pandey, A., Selvakumar, P., Soccol, C. R., and Nigam, P. (1999). Solid State Fermentation for the Production of Industrial Enzymes. Current science 77(1):149-162.
  • [14] Prescott, L. M., Harley, J. P., and Klein, D. A. (2005). Metabolism: Energy, Enzymes, and Regulation. In Microbiology (6th Ed) (pp 156-159, 171-172). New York: McGraw-Hill.
  • [15] Rao, D. M., Swamy, A. V. N., and Siva Rhama Krishna, G. (2007). Bioprocess Technology Strategies, Production, and Purification of Amylases: An Overview. The Internet Journal of Genomics and Proteomics, 2(2): 30-34.
  • [16] Reddy, N. S., Nimmagadda, A., and Sambasiva Roa, K. R. S. (2003). An Overview of the Microbial Alpha-amylase Family. African Journal of Biotechnology, 2(12): 645 – 648.
  • [17] Sanwo, M. M. and Demason, D. A. (1992). Characteristics of Alpha-amylase During Germination of Two High-sugar Sweet Corn Cultivars of Zea mays L. Plant Physiology, 99(3): 1184-1192.
  • [18] Sugita, H., Kawasaki, J., Kumazawa, J., and Deguchi, Y. (2008). Production of Amylase by the Intestinal Bacteria of Japanese Coastal Animals. Letter in Applied Microbiology, 23: 174-178.
  • [19] Sugita, H., Kawasaki, J., and Deguchi, Y. (1997). Production of Amylase by the Intestinal Microflora in Cultured Freshwater Fish. Letter in Applied Microbiology, 24(2), 105-108.
  • [20] Sugita, H., Takahashi, J., and Deguchi, Y. (1992). Production and Consumption of Biotin by the Intestinal Microflora of Cultured Freshwater Fishes. Bioscience, Biotechnology, and Biochemistry, 56: 1678-1679.
  • [21] Sugita, H., Miyajima, C., and Deguchi, Y. (1991). The Vitamin B12 Producing Ability of the Intestinal Microflora of Freshwater Fish. Aquaculture, 92: 267-276.
  • [22] Takeuchi, T. (1991). Digestion and Nutrition of Fish. In Itazawa, Y. and Hanyu, I. (ed). Fish Physiology (pp 67-101). Tokyo: Koseisha Koseikaku (in Japanese).
  • [23] Teotia, S., Khare, S. K., and Gupta, M. N. (2001). An Efficient Purification Process for Sweet Potato Beta-amylase by Affinity Precipitation with Alginate. Enzyme and Microbiological Technology, 28: 792-795.
  • [24] Westerdahl, A., Olsson, J. C., Kjelleberg, S., and Conway, P. (1991). Isolation and Characterization of Turbot (Scophtalmus maimus) Associated Bacteria with Inhibitory Effects Against Vibrio angillarum. Applied and Environmental Microbiology, 57: 2223-2228.
  • [25] J. Li, J. Ni, J. Li, C. Wang, X. Li, S. Wu, T. Zhang, Y. Yu and Q. Yan, Comparative study on gastrointestinal microbiota of eight fish species with different feeding habits, Journal of Applied Microbiology, 117, 6, (1750-1760), (2014).
  • [26] S. Mandal and K. Ghosh, Isolation of tannase‐producing microbiota from the gastrointestinal tracts of some freshwater fish, Journal of Applied Ichthyology, 29, 1, (145-153), (2012).
  • [27] S. Ganguly and A. Prasad, Microflora in fish digestive tract plays significant role in digestion and metabolism, Reviews in Fish Biology and Fisheries, 22, 1, (11), (2012).
  • [28] Qinghui Ai, Houguo Xu, Kangsen Mai, Wei Xu, Jun Wang and Wenbing Zhang, Effects of dietary supplementation of Bacillus subtilis and fructooligosaccharide on growth performance, survival, non-specific immune response and disease resistance of juvenile large yellow croaker, Larimichthys crocea, Aquaculture, 317, 1-4, (155), (2011).
  • [29] Gary Burr, Michael Hume, William H Neill and Delbert M Gatlin III, Effects of prebiotics on nutrient digestibility of a soybean‐meal‐based diet by red drum Sciaenops ocellatus (Linnaeus), Aquaculture Research, 39, 15, (1680-1686), (2008).
  • [30] A. A. Adeoye, E. A. Rotimi, J. E. Udoh, Quantitative Characterization of Farmed African Cat Fish (Clarias gariepinus) in Okitipupa, Ondo State, Nigeria. World Scientific News 47(2) (2016) 329-339
  • [31] Kimiko Uchii, Kazuaki Matsui, Ryuji Yonekura, Katsuji Tani, Takehiko Kenzaka, Masao Nasu and Zen'ichiro Kawabata, Genetic and Physiological Characterization of the Intestinal Bacterial Microbiota of Bluegill (Lepomis macrochirus) with Three Different Feeding Habits, Microbial Ecology, 51, 3, (277), (2006).
  • [32] Ricky Rahmat Maliki Tanjung, Irfan Zidni, Iskandar, Junianto, Effect of difference filter media on Recirculating Aquaculture System (RAS) on tilapia (Oreochromis niloticus) production performance. World Scientific News 118 (2019) 194-208
  • [33] G. I. Izvekova, Hydrolytic activity of enzymes produced by symbiotic microflora and its role in digestion processes of bream and its intestinal parasite Caryophyllaeus laticeps (Cestoda, Caryophyllidea), Biology Bulletin, 10.1134/S1062359006030125, 33, 3, (287-292), (2006).
  • [34] I. Huber, B. Spanggaard, K.F. Appel, L. Rossen, T. Nielsen and L. Gram, Phylogenetic analysis and in situ identification of the intestinal microbial community of rainbow trout (Oncorhynchus mykiss, Walbaum). Journal of Applied Microbiology, 96, 1, (117-132), (2003).
  • [35] I Fernández, F.J Moyano, M Dı́az and T Martı́nez, Characterization of α-amylase activity in five species of Mediterranean sparid fishes (Sparidae, Teleostei), Journal of Experimental Marine Biology and Ecology, 262, 1, (1), (2001).
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