EFFECT OF OLIGOCHITOSANS ON EXPRESSION OF INOS GENE IN EHRLICH ASCITES TUMOUR IN VITRO

• Authors: Jan Ignacak , Maria Wiśniewska-Wrona , Joanna Dulińska-Litewka , Iwona Pałka , Jacek Zagajewski , Antoni Niekraszewicz
• Languages of publication: EN

Abstracts

EN

Oligochitosans obtained through degradation of macromolecules of chitosan with a high degree of deacetylation turned out to be biologically active, contributing to an increase of nitric oxide levels in Ehrlich ascites tumor (EAT) cells through inducing expression of the isoform of inducible nitric oxide synthase (iNOS) gene. An increase of NO levels in EAT cells in the presence of the investigated oligochitosans might contribute to nitrosylation of L-cysteine – an allosteric effector of the M2 isoenzyme of pyruvate kinase (PK), which switches the PK kinase activity, responsible for ATP synthesis, to the histone kinase activity that may participate in histone H1 phosphorylation. Lack of the histone activity of the PK M2 isoenzyme may contribute to decreased histone H1 phosphorylation and thus inhibit EAT cells proliferation.

Keywords

EN

chitosan oligomers; histone H1; iNOS (inducible nitric oxide synthase); nitric oxide

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2012
• Volume: 17
• Pages: 141 - 148

Contributors

author

Jan Ignacak

• Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University

author

Maria Wiśniewska-Wrona

• Institute of Biopolymers and Chemical Fibres ul. M. Skłodowskiej-Curie 19/27, 90-570 Łódź, Poland

author

Joanna Dulińska-Litewka

• Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University

author

Iwona Pałka

• Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University

author

Jacek Zagajewski

• Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University

author

Antoni Niekraszewicz

• Institute of Biopolymers and Chemical Fibers,

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• Document Type: article