PL EN


Preferences help
enabled [disable] Abstract
Number of results
2012 | 17 | 141 - 148
Article title

EFFECT OF OLIGOCHITOSANS ON EXPRESSION OF INOS GENE IN EHRLICH ASCITES TUMOUR IN VITRO

Content
Title variants
Languages of publication
EN
Abstracts
EN
Oligochitosans obtained through degradation of macromolecules of chitosan with a high degree of deacetylation turned out to be biologically active, contributing to an increase of nitric oxide levels in Ehrlich ascites tumor (EAT) cells through inducing expression of the isoform of inducible nitric oxide synthase (iNOS) gene. An increase of NO levels in EAT cells in the presence of the investigated oligochitosans might contribute to nitrosylation of L-cysteine – an allosteric effector of the M2 isoenzyme of pyruvate kinase (PK), which switches the PK kinase activity, responsible for ATP synthesis, to the histone kinase activity that may participate in histone H1 phosphorylation. Lack of the histone activity of the PK M2 isoenzyme may contribute to decreased histone H1 phosphorylation and thus inhibit EAT cells proliferation.
Publisher

Year
Volume
17
Pages
141 - 148
Physical description
Contributors
author
  • Institute of Biopolymers and Chemical Fibres ul. M. Skłodowskiej-Curie 19/27, 90-570 Łódź, Poland
  • Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University
author
  • Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University
  • Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University
  • Institute of Biopolymers and Chemical Fibers,
References
  • Delledonne M., Xia Y.J., Nixon R.A., Lamb C.: (1998) Nitric oxide functions as a signal in plant disease resistance. Nature 394, 585-588.
  • Crawford N.M., Guo F.Q.: (2005) New insights into nitric oxide metabolism and regulatory functions. Trends Plant Sci. 10, 195-200.
  • Lind D.S.: (2004) Arginine and cancer. J.Nutr., 134, 2837S-2841S.
  • Porembska Z., Nyckowski P., Skwarek A., Mielczanek M., Barańczyk-Kuźma A.: (2002) Arginase a marker of cancerogenesis. II. Monitoring of patients after resection of colorectal liver metastases. Pol. Merkuriusz Lek. 13, 286-288.
  • Kruncke K.D., Carlberg C.: (2000) Inactivation of zinc finger transcription factors provides a mechanism for a gene regulatory role of nitric oxide. FASEB J. 14, 166-173.
  • Kruncke K.D.: (2001) Cysteine-Zn2+ complexes: unique molecular switches for inducible nitric oxide synthase-derived NO. FASEB J. 15, 2503-2507.
  • Hmadha A., Bedoya F.J., Sobrino F., Pintado E.: (1999) Methylation dependent gene silencing induced by interleukin-1β via nitric oxide production. J. Exp. Med. 190, 1595-1603.
  • Lipiński P., Starzyński R.R.: (2006) Rola białek IRP (iron metabolism proteins) w regulacji ogólnoustrojowej homeostazy żelaza: lekcje płynące z badań na myszach z nokautem genów IPR1 i IPR2. Post. Hig. Med. Dosw. 60, 322-330.
  • Lindermayr C., Saalbach G., Durner J.: (2005) Proteomic identification of S-Nitrosylated proteins in Arabidopsis. Plant Physiol. 137, 921-930.
  • Weber G.: (1996) Gann Monograph 1, 151-178.
  • Gumińska M., Stachurska M.B., Ignacak J.: (1988) Pyruvate kinase isoenzymes in chromatin extracts of Ehrlich ascites tumour, Morris hepatoma 7777 and normal mouse and rat livers. Biochim. Biophys. Acta, 966, 207-213.
  • Ignacak J., Stachurska M.B.: (2003) The dual activity of pyruvate kinase type M2 from chromatin extracts of neoplastic cells. Comp. Biochem. Phys. 134, 425-433.
  • Ignacak J., Zagajewski J., Pałka I., Wiśniewska-Wrona M., Niekraszewicz A.: (2010) The effect of chitosan on the synthesis of L-S-nitrosocysteine that participates in the regulation of the M2 pyruvate kinase isoenzyme activity associated with Ehrlich ascites tumor cells proliferation in vitro. In: Jaworska M. (ed.) Progress on Chemistry and Application of Chitin and its Derivatives. Polish Chitin Society, Monograph, vol.XV, pp. 149-158.
  • Ignacak J., Wiśniewska-Wrona M., Pałka I., Zagajewski J., Niekraszewicz A.: (2011) Role of chitosan oligomers in regulation of Ehrlich ascites tumor cells proliferation in vitro. In: Jaworska M. (ed.) Progress on Chemistry and Application of Chitin and its Derivatives. Polish Chitin Society, Monograph, vol.XVI, pp. 89-97.
  • Dyer RB, Herzog NK. Isolation of intact nuclei for nuclear extract preparation from a fragile B lymphocyte cell line. BioTechniques 1995;19:192–195.
  • Lowry O.H., Rosebrouggh N.J., Farr A.L., Randal R.J.: (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-273.
  • Nagano T.: (1999) Practical methods for detection of nitric oxide. Luminescence, 14, 283-290.
  • Ignacak J., Dulińska-Litewka J., Pałka I., Wiśniewska-Wrona M., Niekraszewicz A.: (2009) The effect of microcrystalline chitosan on the activity of pyruvate kinase M2 isoenzyme involved in regulating proliferation of Ehrlich ascites tumor (EAT) Wells in vitro. In: Jaworska M. (ed.) Progress on Chemistry and Application of Chitin and its Derivatives. Polish Chitin Society, Monograph, vol. XIV, 111-120.
  • Jerzmanowski A., Cole R.D.:(1993) Histone H1 in modulation of chromatin transcriptional activity. Acta Biochem. Pol. 40, 17-22.
Document Type
article
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.psjd-2ba79e01-aaef-4ed6-b733-2b8609ac3c4d
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.