Preferences help
enabled [disable] Abstract
Number of results
2020 | 29 | 3 | 185-197
Article title

Application of wood rot wild mushrooms in bioethanol production from sawdust of sawmills of Oromia Forest and Wildlife Enterprise, Ethiopia

Title variants
Languages of publication
In this research sawdust samples of Ecalyptus globulus and Cupressus lusitanica were evaluated for bioethanol productions. The sawdust samples were first pretreated with three white rot fungi alone and also by combining the white rot fungi with mild NaOH and steam. Both the fungal and combined pretreated samples were then hydrolyzed with hydrolytic enzymes from three cellulolytic wood rot fungi. Finally, the resulting sugars were fermented into bioethanol using S. cerevisae in anaerobic conditions. Results obtained, in general, indicated that bioethanol amount produced in all cases of sawdust management was significantly higher than the amount obtained from the un-pretreated sawdust samples (p<0.05). In both fungal alone and combined pretreated sawdust samples, higher ethanol yield was obtained from E. globulus than from C. lusitanica. Similarly, combination with NaOH showed better bioethanol yield over combination with steam. The highest alcohol concentration was obtained when pretreated NaOH-006-2G and hydrolyzed with enzymes from 033-1G and followed by results when pretreated with 005-1G and 003-2G, respectively, and hydrolyzed with enzymes from 033-1G.
Physical description
  • Ethiopian Environment and Forest Research Institute, Forest Products Development and Innovation Research and Training Center, Addis Ababa, Ethiopia
  • [1] Ezeoha, S.L., Anyanwu, C.N. and Nwakaire, J.N. (2017). The prospects, impacts and research challenges of enhanced cellulosic ethanol production: a review. Nigerian Journal of Technology 36 (1): 267-275.
  • [2] Valentine, J., Clifton-brown, J., Hastings, A., Robson, P., Allison, G. and Mith, P. (2012). Food vs. fuel: the use of land for lignocellulosic ‘next generation’ energy crops that minimize competition with primary food production. GCB Bioenergy 4: 1-19.
  • [3] Saini, J.K., Saini, R., and Tewari, L. (2015). Lignocellulosic agriculture wastes as biomass feedstocks for second-generation bioethanol production: concepts and recent developments. 3 Biotech 5: 337-353.
  • [4] Branco, R.H.R, Serafim, L.S. and Xavier, A.M.R.B. (2019). Second Generation Bioethanol Production: On the Use of Pulp and Paper Industry Wastes as Feedstock: Review. Fermentation 5, 4.
  • [5] Robak, K. and Balcerek, M. (2018). Review of Second Generation Bioethanol Production from Residual Biomass. Food Technol. Biotechnol. 56 (2): 174-187.
  • [6] Mishra, S., Singh, P.K., Dash, S. and Pattnaik, R. (2018). Microbial pretreatment of lignocellulosic biomass for enhanced biomethanation and waste management. 3 Biotech. 8: 458.
  • [7] Trevorah, R.M. and Othman, M.Z. (2015). Alkali pretreatment and enzymatic hydrolysis of Australian timber mill Sawdust for biofuel production. J. Renewable Energy, Article ID 284250, 9 pages.
  • [8] Safarian, S. and Unnthorsson, R. (2018). An Assessment of the Sustainability of lignocellulosic bioethanol production from wastes in Iceland. Energies 11: 1493.
  • [9] Amezcua-Allieri, M.A., Durán, T.S. and Aburto, J. (2017). Study of Chemical and Enzymatic Hydrolysis of Cellulosic Material to Obtain Fermentable Sugars. Journal of Chemistry, Article ID 5680105.
  • [10] Ibrahim, M.M., El-Zawawy, W.K., Abdel-Fattah, Y.R., Soliman, N.A. and Agblevor, F.A. (2011). Comparison of alkaline pulping with steam explosion for glucose production from rice straw. Carbohydrate Polymers 83: 720-726.
  • [11] Rojo, E., Alonso, M.V., Dom´ınguez, J.C., Saz-Orozco, B.D., Oliet, M. and Rodriguez, F. (2013). Alkali treatment of viscose cellulosic fibers from eucalyptus wood: structural, morphological, and thermal analysis. J. Appl. Polymer Sci. 130(3): 2198-2204.
  • [12] Mirahmadi, M., Kabir, M.M., Jeihanipour, A., Karimi, K. and Taherzadeh, M.J. (2010). Alkaline pretreatment of spruce and birch to improve bioethanol and biogas production. BioResources 5(2): 928-938.
  • [13] Kamdem, I., Jacquet, N., Tiappi, F.M., Hiligsmann, S., Vanderghem, C., Richel, A., Jacques, P. and Thonart, P. (2015). Comparative biochemical analysis after steam pretreatment of lignocellulosic agricultural waste biomass from Williams Cavendish banana plant (Triploid Musa AAA group). Waste Management and Research 33 (11): 1022-1032.
  • [14] Alvira, P., Tomás-Pejó, E., Ballesteros, M. and Negro, M.J. (2010). Pretreatments technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review. Bioresour. Technol. 101: 4851-4861.
  • [15] Qin, D., Ren, H., Zhang, L. and Li, Z. (2015). Enhancing enzymatic digestibility of bamboo by fungal pretreatment. Wood Research 60(1): 25-32.
  • [16] Nazarpour, F., Abdullah, D.K., Abdullah, N. and Zamiri, R. (2013). Evaluation of biological pretreatment of rubber wood with white rot fungi for enzymatic hydrolysis. Materials 6: 2059-2073.
  • [17] Anwar, Z., Gulfraz, M. and Muhammad, I. (2014). Agro-industrial lignocellulosic biomass a key to unlock the future bio-energy: A brief review. J. Rad. Res. and Appl. Sci. 7: 163-173.
  • [18] Alexandropoulou, M., Antonopoulou, G., Ntaikou, I. and Lyberatos, G. (2017). Fungal Pretreatment of willow sawdust with Abortiporus biennis for anaerobic digestion: Impact of an external nitrogen source. Sustainability 9: 130.
  • [19] Martín-Sampedro, R., López-Linares, J.C., Fillat, U., Gea-Izquierdo, G., Ibarra, D., Castro, E. and Eugenio, M.E. (2017). Endophytic fungi as pretreatment to enhance enzymatic hydrolysis of Olive tree pruning. BioMed Research International, Article ID 9727581.
  • [20] Yu, J., Zhang, J., He, J., Liu, Z. and Yu, Z. (2009). Combinations of mild physical or chemical pretreatment with biological pretreatment for enzymatic hydrolysis of rice hull. Bioresour Technol. 100(2): 903-908.
  • [21] Ma F., Yang, N., Xu, C., Yu, H., Wu, J. and Zhang, X. (2010). Combination of biological pretreatment with mild acid pretreatment for enzymatic hydrolysis and ethanol production from water hyacinth. Bioresour Technol. 101: 9600-9604.
  • [22] Megersa, S., Gure, A., Alemu, M. and Feleke, S. (2017a). Qualitative assays and quantitative determinations of laccases of white rot fungi from plantation and natural forests of Arsi forest enterprise, Ethiopia. World Scientific News 67(2): 303-323.
  • [23] Megersa, S., Gure, A., Feleke, S. and Alemu, M. (2017b). Characterization of MnP and LiP from white rot fungi of plantation and natural forests of Arsi forest enterprise. IJPSS 7(5): 6-25.
  • [24] Megersa, S. and Gure, A. (2018). Qualitative assays and quantitative determinations of cellulolytic enzymes of wood rot fungi. American-Eurasian J. Agric. and Environ. Sci. 18 (6): 347-357.
  • [25] Altaf, S.A., Umar, D.M. and Muhammad, M.S. (2010). Production of xylanase enzyme by Pleurotus eryngii and Flamulina velutipes grown on different carbon sources under submerged fermentation. W. Appl. Sci. J. 8: 47-49.
  • [26] Ogunbayo, A.O, Olanipekun, O.O., and Babatunde D.E. (2016). Effect of pretreatment method on the hydrolysis of corn cob and sawdust. Anadolu Univ. J. of Sci. and Technology - A - Appl. Sci. and Eng. 17 (5): 795-811.
  • [27] Hussain, A., Shrivastav, A., Jain, S.K., Baghel, R.K., Rani, S. and Agrawale, M.K. (2012). Cellulolytic enzymatic activity of soft rot filamentous fungi Paecilomyces variotii. Advances in Biores. 3(3): 10-17.
  • [28] Salvachúa, D., Prieto, A., Lopes-Albelaiaras, M., Luchau, T., Martinezs, A.T. and Martinez, M.J. (2011). Fungal pretreatment: an alternative in second generation ethanol from wheat straw. Bioresour. Technol. 102(16): 7500-7506.
  • [29] Nwakaire, J.N., Ezeoha, S.L. and Ugwuishiwu, B.O. (2013). Production of cellulosic ethanol from wood sawdust. Agri. Eng. Int.: CIGR J. 15(3): 136-140.
  • [30] Udhayaraja, P. and J.S. Narayanan. (2012). Optimization for production of bioethanol using sorghum stovar by Saccharomyces cerevisiae. International Journal of Research in Pure and Applied Microbiology 2 (4): 64-67.
  • [31] Ezeoha, S.L., Anyanwu, C.N., and Nwakaire, J.N. (2017). The prospects, impacts, and research challenges of enhanced cellulosic ethanol production: Review. Nigerian Journal of Technology 36 (1): 267-275.
  • [32] Etonihu, A.C. and Idoko, O. (2014). Bioethanol production from lignocellulosic biomass wastes by fermentation of the hydrolyzates. Standard Sci. Res. Essays 9: 431-437.
  • [33] Kathiresan, K., Saravanakumar, K. and Senthilraja, S. (2011). Bio-ethanol production by marine yeasts isolated from coastal mangrove sediment. Int. Multidi. Sci. R. J. 1(1):19-24.
  • [34] Ali, E.N. and Jamaludin, M.Z. (2015). Possibility of producing ethanol from Moringa oleifera pod husk. J. Advanced Res. Design 5(1): 1-9.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.