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2017 | 72 | 141-149
Article title

Probiotic Bacillus sp. environmental strains as a component of improved dishwasher cleaning product

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The use of probiotics has become a mainstream application in pharmaceutical, food, cosmetic and cleaning technologies. Probiotic microorganisms seem to be a remedy for the problem of undesired, persistent microbiological contamination or uncontrolled biofilm formation in the automatic household equipments, dedicated to the undirect contact with food. We propose the mixture of commonly widespread and bio-safe environmental probiotic Bacillus sp. strains as a component of an improved cleaning product for automatic dishwashers. Our design of probiotic-improved cleaning product combines the chemical detergent formula and probiotic Bacillus sp. preparation in two separate pockets of self-soluble cleaning capsule. To pinpoint the validity of an improved probiotic-based cleaning product design, we present a case study of the microflora from the chosen localizations of an old household dishwasher. The swab collecting, followed by bacterial cultivation and MALDI-TOF identification revealed Enterobacter cloacae and Pseudomonas oleovorans forming the major inner microflora of an old household dishwasher, where a commonly available dishwashing products were used. We applied probiotic Bacillus sp. improved cleaning product to a model dishwasher in a conventional cleaning cycle and conducted the before-after trial to identify the inner microflora in chosen localizations. Using standard microbiological Gram-staining method with microscopic observation, followed by MALDI-TOF identification we confirmed, that Bacillus sp. replaced Acinetobacter sp., residing formerly model dishwasher filter and water drain. Conducted experiments have shown the potential for use of the chosen probiotic Bacillus sp. strains mixture as an addition for encapsulated formula of cleaning product, to provide microbiologically safe and controllable conditions during the conventional dishwasher cleaning cycle.
Physical description
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