Evaluation of phytochemical constituents of Methanol extract of Moringa oleifera Lam. whole leaf by Gas Chromatography-Mass Spectrometry and Fourier transform infrared spectroscopy analysis

• Authors: Victor Uchechukwu Okechukwu , Sunday Onyekwere Eze , Daniel Omeodisemi Omokpariola , Janefrancess Chinemerem Okereke
• Languages of publication: EN

Abstracts

EN

The research study evaluated the phytochemical constituents of Methanol extracts of Moringa oleifera whole leaf by Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FT/IR) analysis. The leaves was washed, air dried for 2 weeks, then ground into a fine powder and extracted using methanol by maceration for 24 hours using standard procedures. After the contact time elapsed, the solvent filtered to recover the extract. The functional groups and the chemical constituents of the methanol extract of Moringa oleifera leaves investigated using Fourier transform infrared spectroscopy (FT/IR) and Gas Chromatography-Mass Spectrometry (GC-MS) respectively. The FT/IR analysis confirmed the presence of O-H, C=C, C-H, C-O, CH3 and C=C-H bond stretching functional groups, which indicates that the substance is an aliphatic alcohol, ester, aldehyde and carboxylic acid. However, the fingerprint region had a pattern that is specific for every molecule, the presence of -OH function and N-O stretch; suggest that or alcohols and nitrogen or aromatic or aliphatic phenols containing molecules are major components of the Moringa oleifera leaf studied. GC-MS analysis of the extract reveals the identification of twenty compounds, in which two compounds were identified in each peak. N,N'-Pentamethylenebis[s-3-aminopropyl thiosulfuric acid and 2-Myristynoyl pantetheine (100%), 2-Myristynoyl pantetheine and Deoxyspergualin (92.05%), 5-Octadecenal and 9-Hexadecenoic acid (27.94%). N,N'-Pentamethylenebis[s-3-aminopropyl thiosulfuric acid and Pentetic Acid are the major phytoconstituents. Most of the compounds in the list are bioactive and possess medicinal properties, which further justify the application of Moringa oleifera traditional plant in the discovery of novel therapeutics.

Keywords

EN

FT-IR analysis; GC-MS analysis; Moringa oleifera; methanol extract; phytochemical constituents

Discipline

• BIOCHEMISTRY: BIOCHEMISTRY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2021
• Volume: 37
• Pages: 18-30

Contributors

author

Victor Uchechukwu Okechukwu

• Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University, Awka, Nigeria

author

Sunday Onyekwere Eze

• Department of Chemistry, Abia State University Uturu, Nigeria

author

Daniel Omeodisemi Omokpariola

• Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University, Awka, Nigeria

author

Janefrancess Chinemerem Okereke

• Department of Chemistry, Abia State University Uturu, Nigeria

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• Document Type: article