Preparation, chemical components and biological activities of essential oil from Licorice seeds

• Authors: Xuhui Qin , Li Le , Fujunrui Jiang , Jianping Yong , Danian Tian
• Languages of publication: EN

Abstracts

EN

The essential oil from licorice seeds was prepared and investigated using gas chromatography-mass spectrometry (GC-MS), resulting in the identification of 47 beneficial components. The oil was found to be abundant in fatty acids: including linoleic acid (43.87%), palmitic acid (10.43%), oleic acid (4.51%), stearic acid (1.83%), and palmitoleic acid (1.25%). The oil’s physicochemical properties revealed an acid value of 0.066 mg/g, a peroxide value of 7.267 g/100g, an iodine value of 37.231 g/100g, and a saponification value of 186.823 mg/g, indicating that it can be regarded as a premium edible oil. The antioxidant activity of this oil showed that it has a antioxidant capacity with a free radical scavenging rate of up to 38.53% at a concentration of 15 mg/mL. The in vitro anticancer activities against lung cancer A549 cell lines and cervical cancer Hela cell lines showed that it inhibited the cancer cell lines with cell viability of 61% and 56% at a concentration of 100 mg/mL, respectively with a dose-depedent manner. This results indicate that the oil can be considered to be used as edible oil or potential applications in the field of food and medicine.

Keywords

EN

Licorice seeds; anticancer; antioxidant; chemical components analysis; essential oil; preparation

Discipline

• BIOCHEMISTRY: BIOCHEMISTRY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2024
• Volume: 54
• Pages: 45-57

Contributors

author

Xuhui Qin

• Ningxia Medical University, Yinchuan, Ningxia, China

author

Li Le

• Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, China

author

Fujunrui Jiang

• Ningxia Medical University, Yinchuan, Ningxia, China

author

Jianping Yong

• Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, China

author

Danian Tian

• Ningxia Medical University, Yinchuan, Ningxia, China

References

• [1] National Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China. A Part. Beijing, China Pharmaceutical Science and Technology Press, 2020.
• [2] Shang, Zhanpeng, Chenrui Liu, Xue Qiao, and Min Ye. Chemical analysis of the Chinese herbal medicine licorice (Gan-Cao): An update review. Journal of Ethnopharmacology, 299, 115686 (2022)
• [3] Wei-wei Han, Dong-jun Fang, Lu-jun Li, and Yan Liu. Research progress on chemical constituents and biological activities of Glycyrrhiza uralensis Fisch. Chemical Engineer, 36(2), 56-58 (2022)
• [4] Chen Xiang, Xue Qiao, Min Ye, and De-an Guo. Classification and distribution analysis of components in Glycyrrhiza using licorice compounds database. Journal of Pharmacy 47(8), 1023-1030 (2012)
• [5] Yang, R., Yuan, B. C., Ma, Y. S., Zhou, S., and Liu, Y. The anti-inflammatory activity of licorice, a widely used Chinese herb. Pharmaceutical Biology 55(1), 5-18 (2017)
• [6] Zuo, J., Meng, T., Wang, Y. and Tang, W. A review of the antiviral activities of glycyrrhizic acid, glycyrrhetinic acid and glycyrrhetinic acid monoglucuronide. Pharmaceuticals 16(5), 641 (2023).
• [7] Park, M., Horn, L., Lappi, V., Boxrud, D., Hedberg, C. and Jeon, B. Antimicrobial synergy between aminoglycosides and licorice extract in Listeria monocytogenes. Pathogens 11(4), 440 (2022)
• [8] Wang, Zhi Y., and Daniel W. Nixon. Licorice and cancer. Nutrition and Cancer, 39(1), 1-11 (2001)
• [9] Kim, H.J., Seo, J.Y., Suh, H.J., Lim, S.S. and Kim, J.S. Antioxidant activities of licorice-derived prenylflavonoids. Nutrition Research and Practice 6(6), 491 (2012)
• [10] Feihua Wu, Zhigui Jin, Jian Jin. Hypoglycemic effects of glabridin, a polyphenolic flavonoid from licorice, in an animal model of diabetes mellitus. Molecular Medicine Reports 7(4), 1278-1282 (2013)
• [11] Armanini, D., Mattarello, M.J., Fiore, C., Bonanni, G., Scaroni, C., Sartorato, P. and Palermo, M. Licorice reduces serum testosterone in healthy women. Steroids, 69(11-12), 763-766 (2004)
• [12] Wu, S.Y., Wang, W.J., Dou, J.H. and Gong, L.K. Research progress on the protective effects of licorice-derived 18β-glycyrrhetinic acid against liver injury. Acta Pharmacologica Sinica 42(1), 18-26 (2021)
• [13] Ma, X., Sun, Z., Chen, H., Cao, L., Zhao, S., Fan, L., Zhao, C., Yin, S. and Hu, H. 18β-glycyrrhetinic acid suppresses Lewis lung cancer growth through protecting immune cells from ferroptosis. Cancer Chemotherapy and Pharmacology 1-11 (2024)
• [14] Ding, Y., Brand, E., Wang, W. and Zhao, Z. Licorice: resources, applications in ancient and modern times. Journal of Ethnopharmacology 298, 115594(2022).
• [15] Wang, L., Zhang, K., Han, S., Zhang, L., Bai, H., Bao, F., Zeng, Y., Wang, J., Du, H., Liu, Y. and Yang, Z. Constituents isolated from the leaves of Glycyrrhiza uralansis and their anti-inflammatory activities on LPS-induced RAW264. 7 cells. Molecules, 24(10), 1923 (2019)
• [16] Li-Yao Wang. Study on the Chemical Constituents in Ethyl Acetate Layer of Leaf Extract from Glycyrrhiza ural. Lanzhou University, 2020.
• [17] Docimo T, Celano R, Lambiase A, Di Sanzo R, Serio S, Santoro V, Coccetti P, Russo M, Rastrelli L, Piccinelli AL. Exploring Influence of Production Area and Harvest Time on Specialized Metabolite Content of Glycyrrhiza glabra Leaves and Evaluation of Antioxidant and Anti-Aging Properties. Antioxidants, 13(1), 93 (2024)
• [18] Deng Yinmei. Study on the Extraction Technology of Total Flavonoids and Potential Basis for Prostatitis from Glycyrrhiza Leaves. Central South University for Nationalities, 2008.
• [19] Ye, Rigui, Yu-Hong Fan, and Chao-Mei Ma. Identification and Enrichment of α-Glucosidase-Inhibiting Dihydrostilbene and Flavonoids from Glycyrrhiza uralensis Leaves. Journal of Agricultural and Food Chemistry, 65(2), 510-515 (2017)
• [20] da Silva, A. P., Silva, N. D. F., Andrade, E. H. A., Gratieri, T., Setzer, W. N., Maia, J. G. S., and da Silva, J. K. R. Tyrosinase inhibitory activity, molecular docking studies and antioxidant potential of chemotypes of Lippia origanoides (Verbenaceae) essential oils. PloS ONE, 12(5), e0175598 (2017)
• [21] La, J., Kim, M. J., and Lee, J. Evaluation of solvent effects on the DPPH reactivity for determining the antioxidant activity in oil matrix. Food Science and Biotechnology, 30, 367-375 (2021)
• [22] Dachev, M., Bryndová, J., Jakubek, M., Moučka, Z., and Urban, M. The effects of conjugated linoleic acids on cancer. Processes, 9(3), 454 (2021)
• [23] Zahir, E., Saeed, R., Hameed, M. A., and Yousuf, A. Study of physicochemical properties of edible oil and evaluation of frying oil quality by Fourier Transform-Infrared (FT-IR) Spectroscopy. Arabian Journal of Chemistry, 10, S3870-S3876 (2017)
• [24] Jingzun Wang, and Tim Wang. How to Interpret Infrared (IR) Spectra. University Chemistry, 6, 90-97 (2016)

• Document Type: article