In Vitro Micropropagation of Vernonia cinerea (L.) Less. - An important medicinal herb
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In vitro micropropagation was achieved from Nodal and leaf explants of Vernonia cinerea L. on MS medium supplemented with different concentrations of BAP (1.0 to 3.0 mg/l), GA3 (1.0 to 5.0 mg/l) and KN (0.5 mg/l) for direct shoot induction. Maximum numbers of shoots were obtained from nodal and leaf explants at BAP (2.5 mg/l) GA3 (2.0 mg/l) and KN (0.5 mg/l) respectively. The multiple shoots elongation and their development were better on MS medium supplemented with 1.5 mg/l BAP and 3.0 mg/l GA3 for both the explants. The regenerated shoots were successfully rooted on MS medium supplemented with IBA 2.0 mg/1, after sequential hardening, survival rate was 91%. The in vitro raised plantlets were acclimatized and established successfully in the field.
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