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2022 | 167 | 28-40

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Phytochemical screening and bioassay of the ethanolic extracts of Ocimum basilicum and Commiphora kerstingii leaves


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Phytochemical screening, and antioxidant activities of ethanolic crude extracts of the leave Ocimum basilicum and Commiphora kerstingii were investigated. Phytochemical screening was conducted using standard qualitative techniques, while the essential oil was extracted from the Ocimum basilicum and Commiphora kerstingii leaves by modified steam distillation apparatus. The plant extract was tested to investigate its chemical composition and antioxidant activity. Phytochemical analysis of the ethanolic crude extract showed presence of major classes of phytochemicals i.e Essential oil, Alkaloids, Tannins, Saponins, Flavonoids, Carbohydrates, Proteins, Terpenoids and Anthraquinones, phytosterol in Ocimum bacilicum, glycoside absent while all the tested phytochemicals were present in Commiohora kerstingii except phytosterol. The antioxidant activity of the ethanolic crude extract was screened using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide free radical scavenging assay using ascorbic acid (standard drug) as reference. Antioxidant activities test using both the DPPH and and hydrogen peroxide free radical scavenging method reveals that all the ethanolic extract examined showed positive effect at difference concentration. Using DPPH radicals scavenging method, At a concentration of 10 μL/mL, the Ocimum basilicum extract significantly scavenged 87.68%, Commiphora kerstingii scavenged 81.72% and ascorbic acid (standard) scavenge 91.24%. Against hydrogen peroxide free radical scavenging,at a concentration of 10 μL/mL, Ocimum basilicum leaves extract exhibited a maximum of 90.10% of activity, Commiphora kerstingii scavenged 86.34% and ascorbic acid (standard drug) scavenged 90.60%.






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  • Department of Chemistry, Modibbo Adama University, Yola, Adamawa State, Nigeria
  • Department of Chemistry, Modibbo Adama University, Yola, Adamawa State, Nigeria
  • Department of Petroleum Chemistry and Engineering, American University of Nigeria, Yola, Adamawa State, Nigeria
  • Department of Chemistry, Adamawa State University, Mubi, Adamawa State, Nigeria
  • Department of Biological Sciences, Adamawa State University, Mubi, Adamawa State, Nigeria


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