Preferences help
enabled [disable] Abstract
Number of results
2018 | 21 | 164-170
Article title

The Influence of Concentration of Acetic Acid and Pepsin Enzyme in Nilem Fish Skin Collagen Extractionto the Amount of Rendement Produced

Title variants
Languages of publication
The added value of nilem fish skin needs to be increased. The purpose of this study is to determine the concentration of acetic acid solution combined with the pepsin enzyme in the extraction of collagen from nilem fish skin that is necessary to obtain the highest yield/renderment. The study employed an experimental research method that used a completely randomized factorial design. The first treatment is the concentration of acetic acid solution. This consists of three levels, namely 0.5M, 0.7M and 0.9M. The second treatment is the concentration of the enzyme pepsin. This in turn consists of three levels, namely 0.5 %, 1.0 % and 1.5% (weight / weight). The parameters observed were collagen renderment. The results showed that the combination treatment concentration of 0.7 M solution of acetic acid by the pepsin enzyme at 1.0%, in the extraction of collagen from fish skin, produce the highest yield compared to other combinations. The renderment yield is 6.18%.
Physical description
  • Department of Fishery, Universitas Padjadjaran, West Java, Indonesia
  • Department of Fishery, Universitas Padjadjaran, West Java, Indonesia
  • Department of Fishery, Universitas Padjadjaran, West Java, Indonesia
  • Laboratory of Fishery Products Processing, Univesitas Padjadjaran, West Java, Indonesia
  • [1] Aberoumand A. 2012. Comparative Study Between Different Extraction Methods of Collagen from Fish and its Properties. World Applied Sciences Journal 16 (3): 316-319.
  • [2] Bama,P., Vijayalakshimi, M., Jayasimman, R., Kalaichelvan PT, Deccaraman, M., and Sankaranarayanan, S. 2010. Extraction of collagen from cat fish (Tachysurus maculatus) by pepsin digestion and preparation and characterization of collagen chitosan sheet. International Journal of Pharmacy and Pharmaceutical Sciences Vol.2: 133-137.
  • [3] Bhagwat PK and Dandge PB. 2016. Isolation, characterization and applications of fi sh valorizable scale collagen in food and agriculture industries. Biocatalysis and Agricultural Biotechnology 7: 234-240.
  • [4] Hema GS, Shyni K, Mathew S, Anandan R, Ninan G, and Lakshmanan PT 2013. A simple method for isolation of fish skin collagen-biochemical characterization of skin collgagen extracted from Albacore Tuna (Thunnus Alalunga), Dog Shark (Scoliodon Sorrakowah), and Rohu (Labeo Rohita). Annals of Biological Research 4 (1): 271-278.
  • [5] Junianto. 2015. The strategic role of fisheries product processing in national development. Oration Scientific, Faculty of Fisheries and Marine Sciences - Padjadjaran University. Jatinangor, Indonesia
  • [6] Junianto, Afrianto, E., Rohimma, R., Rostika. R., Andriani. Y., Handaka, AA and Nurhayati, A. 2015. Commercialization of products made from raw fish nilem as leverage local fish production in West Java.Research Report ALG, Unpad. Bandung
  • [7] Kittiphattanabawon, P., Benjakul, S., Visessaguan, W., Nagai, T., and Tanaka, M. 2005. Characterization of acid-soluble collagen from skin and bone of bigeye snapper (Priacanthus tayenus). Food Chemistry 221: 363-372
  • [8] Liu D, Wei G, Li T, Hu J, Lu N, Regenstein, JM and Zhou P. 2015. Effects of alkaline pretreatments and acid extraction conditions on the acid-soluble collagen from grass carp (Ctenopharyngodon idella) skin. Food Chemistry Vol. 172: 836-843.
  • [9] Sahubawa L and Putra ABN 2011. Effect of acetic acid concentration and extraction time on collagen quality of skin waste of black tilapia fish. Journal of Science Technology, No.1: 16-25
  • [10] Siddiqui, YD, Arief, EM, Yusoff, A., Suzina, AH and Abdullah, SY, 2013. Isolation of pepsin-Solublized (PSC) from the crude collagen extracted from the body wall of sea cucumber (Bohadschia spp). International Journal of Pharmacy and Pharmaceutical Sciences Vol. 5: 555-559.
  • [11] Suseno SH, Suptijah P and Wahyuni D S. 2004. Influence of addition of Nilem fish flesh (Ostheochilus hasselti) on Making Simping As Snack Food. Technology Bulletin of Fishery Products. Vol. VII no. 1. Pages: 44-55.
  • [12] Veeruraj A., Arumugam M., Ajithkumar T and Balasubramanian T. 2015. Isolation and characterization of collagen from the outer skin of squid (Doryteuthis singhalensis). Food Hydrocolloids 43: 708 - 716.
  • [13] Zhang, J., Duan, R., Ye, C., & Konno, K. (2010). Isolation and characterization of collagens from scale of silver carp (Hypophthalmichthys molitrix). Journal of Food Biochemistry, 34 (6), 1343-1354.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.