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2018 | 107 | 108-124
Article title

Biochemical and molecular studies of Jatropha curcas L. - Biofuel species

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EN
Abstracts
EN
Fossil fuels plays vital role in the development of the nation. The limitation of the Non-renewable energy required alternative energy sources such as solar, wind energy, hydro energy and biomass energy etc., and these renewable energies are eco-friendly in nature. The study has conducted for the determination of fatty acid profile in Jatropha curcas oil, determination of restriction sites in genomic DNA of Jatropha curcas, comparison of DGAT gene sequence alignment of different plants using Clustal W software. and PCR amplification of DGAT gene. The results confirmed that the solvent extraction produced high quality oil. From the thin layer chromatography plate, the saturated fatty acid like Linoleinic acid (0.14), Palmatic acid (0.38), Stearic acid (0.72), and unsaturated fatty acid like and Oleic acid (0.90) were identified. The genomic DNA was restriction digested with EcoRI, BamHI, PstI, HindIII. The observed that restriction bands for EcoRI was 3 sites and for Hind III was 1 site respectively. The amplification of gene encoding DGAT gene from the genomic DNA was carried out by polymerase chain reaction and also by bioinformatics software and was found to be approximately 2 kb size. Our study makes initial step for altering of the Jatropha seed oil for enhancing the oil content level.
Year
Volume
107
Pages
108-124
Physical description
Contributors
  • Department of P.G. Studies and Research in Biotechnology, Gulbarga University, Kalaburagi, Karnataka, 585106, India
author
  • Department of P.G. Studies and Research in Biotechnology, Gulbarga University, Kalaburagi, Karnataka, 585106, India
author
  • Department of P.G. Studies and Research in Biotechnology, Gulbarga University, Kalaburagi, Karnataka, 585106, India
author
  • Department of P.G. Studies and Research in Biotechnology, Gulbarga University, Kalaburagi, Karnataka, 585106, India
References
  • [1] Banapurmath NR, PG Tewari, RS Hosmath (2008). Performance and emission characteristics of a DI compression ignition engine operated on Honge, Jatropha and sesame oil methyl esters. Renewable Energy. 33: 1982-1988.
  • [2] Baud S, Lepiniec L (2009). Regulation of de novo fatty acid synthesis in maturing oilseeds of Arabidopsis. Plant Physiol Biochem Jun; 47(6): 448-55.
  • [3] Cagliari, A., Margis, R., dos Santos Maraschin, F., Turchetto-Zolet, A. C., Loss, G., and Margis-Pinheiro, M. (2011). Biosynthesis of triacylglycerols (TAGs) inplants and algae. Int. J. Plant Biol. 2, 10.
  • [4] Doyle J. J. and Doyle J. C. (1990). Isolation of plant DNA from fresh tissue. Focus, 12: 13-15.
  • [5] Foidl N, Foidl G, M Sanchez, M Mittelbach, S Hackel (1996). Jatropha curcas L. as a resource for the production of Biofuel in Nicaragua. Bioresour. Technol. 58: 77-82.
  • [6] Giannoulia K, Haralampids K, Poghosyan Z, Murphy DJ, Hatzopoulos P (2000). Differential expression of diacylglycerol acyltransferase (DGAT) genes in olive tissues. Biochemical Society Transactions 28, 695–697.
  • [7] Gressel J. (2008). Transgenics are imperative for biofuel crops - Review. Plant Sci. 174: 246-263.
  • [8] Hobbs DH, Chaofu L, Hills MJ (1999). Cloning of a cDNA encoding diacylglycerol acyltransferase from Arabidopsis thaliana and its functional expression. FEBS Lett 452: 145–149
  • [9] Ilaiyaraj N., A P Rajarani and I M Santha (2008). Cloning and characterization of diacylglycerol acyltransferase (DGAT) cDNA sequence from Brassica juncea cv. Pusa Bold. Indian journal of Biochemistry and Biophysics: Vol. 45, 30-36.
  • [10] Nykiforuk Cory L, Andre Laroche André Laroche R. J. Weselake (1999). Isolation and sequence analysis of a novel cDNA encoding a putative diacylglycerol acyltransferase from a microspore-derived cell suspension culture of Brassica napus L. cv Jet Neuf. Plant physiology 120: 99-123.
  • [11] Quettier, A. L., and P. J. Eastmond (2009). Storage oil hydrolysis during early seedling growth. Plant Physiol. Biochem. 47: 485-490 .
  • [12] Sarin R, M Sharma, S Sinharay, RK Malhotra (2007). Jatropha-Palm biodiesel blends: An optimum mix for Asia. Fuel 86: 1365-1371.
  • [13] Tamalampundi S, MR Talukder, S Hama, T Numata, A Kondo, H Fukuda (2008). Enzymatic production of biodiesel from Jatropha oil: A comparative study of immobilised-whole cell and commercial lipases as a biocatalyst. Biochem. Engineering J. 39: 185-189.
  • [14] Zou J, Wei Y, Jako C, Kumar A, Selvaraj G, Taylor DC (1999). The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene. Plant J. Sep; 19(6): 645-53.
Document Type
article
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YADDA identifier
bwmeta1.element.psjd-11453df4-4d9c-41b3-977b-f2d0bad43532
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