PL EN


Preferences help
enabled [disable] Abstract
Number of results
2016 | 32 | 73-83
Article title

Direct regeneration of Withania somnifera (L.) Dunal – A medicinal plant

Content
Title variants
Languages of publication
EN
Abstracts
EN
Plants have been an important source of medicine for thousands of years. Medicinal plants are the most important source of life saving drugs. Even today, the WHO estimates that up to 80 per cent of people still rely mainly on traditional remedies such as herbs for their medicines. Medicinal plants are resources of new drugs. Approximately one quarter of prescribed drugs contain plant extracts or active ingredients obtained from plant substances. Medicinal plants are the most important source of life saving drugs for the majority of the world’s population. The biotechnological tools are important to select, multiply and conserve the critical genotypes of medicinal plants. In-vitro regeneration holds tremendous potential for the production of high-quality plant-based medicine. An efficient protocol was developed for high frequency and rapid regeneration was achieved in Withania somnifera (L.) Dunal. Invitro cultures were initiated by meristematic explants on M.S–B5 medium with various growth hormones individually as well as combinations. Highest shoot multiplication rates were observed when explants grown in the medium supplemented with 2.0 mg/l of BAP. M.S–B5 medium fortified with 1.0 mg/l BAP was found suitable for optimum growth and elongation of shoots. The best rooting response were observed when the medium containing 1.0 mg/l of IBA. The rooted plantlets were successfully established in soil.
Discipline
Year
Volume
32
Pages
73-83
Physical description
References
  • [1] Akin-Idowu PE, Ibitoye DO, Ademoyegun OT (2009) Tissue culture as a plant production technique for horticultural crops. Afr. J. Biotechnol. 8(16): 3782-3788.
  • [2] Arora, R and S.S. Bhojwani (1989). Invitro propagation and low temperature storage of Saussures lappa C.B. Clarke – an endangered medicinal plant. Pl. Cell Rep., 8: 44-47.
  • [3] Baldi, A., D. Singh and V.K. Dixit, (2008). Dual elicitation for improved production of withaferin – A by cell suspension cultures of Withania somnifera. Appl. Biochem. Biotechnol., 151: 556-564.
  • [4] Farooqi, A.A., and B.S. Sreeramu (2004). Cultivation of medicinal and aromatic plants. University press, India.
  • [5] Gamborg, O.L., R.A. Miller and K. Ojima (1968). Nutrient experiments of suspension culture of soybean root cells. Exp. Cell. Res., 80: 150-158.
  • [6] George, E.E., and R.D. Sherrington (1984). Plant propagation of Tissue Culture Hand Book and Directory of commercial Laboratories. Eversley, Exegetics Ltd., England.
  • [7] Handique and Bora (1999). Invitro regeneration of a medicinal plant – Houttuynia cordata Thunb. From nodal explants. Curr. Sci., 76(9): 1245.
  • [8] Jayakumar, S. and Ramalingam, R. (2013). Influence of Additives on Enhanced in Vitro Shoot Multiplication of Orthosiphon aristatus (Blume) Miq. Notulae Scientia Biologicae, 5, 338-345.
  • [9] Kawata, K., C. Ushida, F. Kawai, M. Manamori and A. Kuriyama (1995). Micropropagation of passion fruit sub cultured multiple shoot primordial-passion fruit leaf culture derived shoot primordial and rooting culture medium optimization for propagation. J. Plant Physiol., 147(2): 281-284.
  • [10] Kolewe ME, Gaurav V, Roberts SC (2008) Pharmaceutically active natural product synthesis and supply via plant cell culture technology. Molecular Pharmaceutics 5: 243-256.
  • [11] Kumar, V., Parvatam, G. and Ravishankar, G.A. (2009) AgNO3 - A Potential Regulator of Ethylene Activity and Plant Growth Modulator. Electronic Journal of Biotechnology, 12, 1-15.
  • [12] Matsuda, H., T. Murakami., A. Kishi and M. Yoshikawa (2000). Structure of withanosides I, II, III, IV, V, VI and VII. New withanolide glycosides from the roots of Indian Withania somnifera Dunal., Bio Organic & Med. Chem., 9: 1499-1507.
  • [13] Mohammed, S. and Ali, M. (2010). Effect of Coconut Water on Callus Growth on Cyamopsis tetragonolobust. Pharmacia, 1, 25-28.
  • [14] Molnar, Z., Virag, E. and Ordog, V. (2011) Natural Substances in Tissue Culture Media of Higher Plants. Acta Biologica Szegediensis, 55, 123-127.
  • [15] Murashige, T., and F. Skoog (1962). A revised medium for rapid growth the bioassay with tobacco tissue culture. Pl. Physiol., 147(2): 270-272.
  • [16] Newman D.J., Cragg G.M. (2012). Natural products as sources of new drugs over the 30 years from 1981 to 2010. Journal of Natural Products 75: 311-335.
  • [17] Newman D.J., Cragg G.M., Snader K.M. (2000). The influence of natural products upon drug discovery. Natural Products Reports 17: 215-234.
  • [18] Pimm S.L., Russell G.J., Gittleman J.L., Brooks T.M. (1995). The future of biodiversity. Science 269: 347-350.
  • [19] Rani. G., and I.S. Grover (1999). Invitro callus induction and regeneration studies in Withania somnifera., Plant Cell Tiss. Org. Cult., 57: 23-27.
  • [20] Raskin I, Ribnicky DM, Komarnytsky S, IIic N, Poulev A, et al. (2002). Plants and human health care in the twenty –first century. Trends in Biotechnology 20: 522-531.
  • [21] Soundar Raj. V., D.H. Tejavathi and B.H.M. Nijalingappa (1989). Shoot tip culture in Dolichous biflours. Curr. Sci., 58: 1385-1388.
  • [22] Sridhar, T.M. (2011). Studies on Plant Regeneration, Phytochemicals and Antimicrobial Activities of Solanum nigrum (L.). Ph.D. Dissertation, Sri Venkateswara University, Tirupati.
  • [23] Thorpe T (2007). History of plant tissue culture. J. Mol. Microbial Biotechnol. 37: 169-180.
  • [24] Verpoorte R (1998). Exploration of nature’s chemodiversity: the role of secondary metabolites as lead drugs for drug development. Drug development Today 3: 232-238.
Document Type
article
Publication order reference
YADDA identifier
bwmeta1.element.psjd-0f5d7084-bb92-483d-a03e-b5802767abff
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.