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2017 | 86 | 3 | 349-356
Article title

Biochemical study of trehalase activity in Aspergillus niger conidia

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EN
Abstracts
EN
Trehalase (α, α-trehalose-1-C-glucohydrolase, EC 3.2.1.28) is a glycoside hydrolase enzyme that catalyzes the conversion of trehalose to glucose. Trehalose hydrolysis by trehalase enzyme is an important physiological process for various organisms, such as fungal spore germination, insect flight, and the resumption of growth in resting cells. So by studying biochemical properties of trehalase we can control the spore germination of harmful fungus, Apergillus niger. Till now trehalase has been reported from many other organisms including plants and animals, Trehalase has been purified and characterized from various organisms such as Saccharomyces cerevisiae, Lentinula edodes, Acidobacterium capsulatum, and Medicago sativa, as well as root nodules of Phaseolus vulgaris, soybean and from the from the seeds of Triticum aestivum. In this study, trehalase was purified from Aspergillus niger grown on cellulosic medium. And this purified trehalase was tested for various requirements it may need for enzymatic activity such as inorganic phosphate and Magnesium ions (Mg2+).
Discipline
Publisher

Year
Volume
86
Issue
3
Pages
349-356
Physical description
Contributors
  • Department of Biochemistry, Panjab University, Sector-25, Chandigarh 160014, India
  • Department of Biochemistry, Panjab University, Sector-25, Chandigarh 160014, India
  • Department of Biochemistry, Panjab University, Sector-25, Chandigarh 160014, India
References
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Document Type
short_communication
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.psjd-0ab52a6c-9bd7-44bd-a7dd-845f34775264
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