Preferences help
enabled [disable] Abstract
Number of results
2017 | 86 | 3 | 338-345
Article title

Biochemical study of trehalase activity in Aspergillus niger conidia

Title variants
Languages of publication
Trehalase (α, α-trehalose-1-C-glucohydrolase, EC is a glycoside hydrolase enzyme that catalyzes the conversion of trehalose to glucose. Trehalose hydrolysis by trehalase enzyme is an important physiological process for various organisms, such as fungal spore germination, insect flight, and the resumption of growth in resting cells. So by studying biochemical properties of trehalase we can control the spore germination of harmful fungus, Apergillus niger. Till now trehalase has been reported from many other organisms including plants and animals, Trehalase has been purified and characterized from various organisms such as Saccharomyces cerevisiae, Lentinula edodes, Acidobacterium capsulatum, and Medicago sativa, as well as root nodules of Phaseolus vulgaris, soybean and from the from the seeds of Triticum aestivum. In this study, trehalase was purified from Aspergillus niger grown on cellulosic medium. And this purified trehalase was tested for various requirements it may need for enzymatic activity such as inorganic phosphate and Magnesium ions (Mg2+).
Physical description
  • Department of Biochemistry, Panjab University, Sector-25, Chandigarh 160014, India
  • Department of Biochemistry, Panjab University, Sector-25, Chandigarh 160014, India
  • Department of Biochemistry, Panjab University, Sector-25, Chandigarh 160014, India
  • [1] Bradford M, M, (1976), Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
  • [2] Creppy, E. E. (2002). Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicology Letters 127(1-3), 19-28.
  • [3] Dagnas, S. & Membre, J. M. (2013). Predicting and preventing mold spoilage of food products. Journal of Food Protection 76(3), 538-551.
  • [4] Filtenborg, O., Frisvad, J. C. & Thrane, U. (1996). Moulds in food spoilage. International Journal of Food Microbiology 33(1), 85-102.
  • [5] Geiser, D. M., Gueidan, C., Miadlikowska, J., Lutzoni, F., Kauff, F., Hofstetter, V., Fraker, E., Schoch, C. L., Tibell, L., Untereiner, W. A. & Aptroot, A. (2006). Eurotiomycetes: Eurotiomycetidae and Chaetothyriomycetidae. Mycologia 98(6), 1053-1064.
  • [6] Keir, G. J., Garfield B., Hansell, D. M., Loebinger, M. R., Wilson, R., Renzoni, E. A., Wells, A. U., Maher T. M. (2014). Cyclical caspofungin for chronic pulmonary aspergillosis in sarcoidosis. Thorax. 69(3), 287-288.
  • [7] Miller G. L. (1959). Use of Dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31, 426-448.
  • [8] Pitt, J.I. & Hocking, A.D. (2009). Fungi and Food Spoilage, Introduction. In: Fungi and Food Spoilage, Third Edition. pp. 1-501 Springer. ISBN 978-0-387-92206-5
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.