PL EN


Preferences help
enabled [disable] Abstract
Number of results
2020 | 144 | 384-396
Article title

Molecular diversity of the bacterial community associated with Acropora digitifera (Dana, 1846) corals on Rancabuaya coastline, Garut District, Indonesia

Content
Title variants
Languages of publication
EN
Abstracts
EN
Bacteria are one of the prokaryotic microorganisms that are symbiotic with coral reefs. The purpose of this study was to determine the diversity of bacterial communities associated with Acropora digitifera corals in the rancabuaya coastline of Garut district, West Java through a metagenomic and cultural approach. Stages of research include tissue isolation using waterpics, isolation of bacterial genomic DNA, sequencing using the NGS (Next Generation Sequencing) method of Hiseq using 16S rRNA V3-V4 region 341F and 806R primers for the metagenomic approach. While the culture approach, carried out inoculation, bacterial cultivation, gram staining, then proceed with the identification of molecular characteristics of DNA with 16S rRNA gene sequences. Dominantly, the results of bacterial identification were obtained as many as 77 species from 10 genera 10 families 10 orders of 10 classes and 4 phylum and unidentified reads of 6%. The results of the identification of 16S rRNA showed that the isolate ACD.P4.PH7.P had a close relationship with the Bacillus flexus strain BF strain zb strain with a similarity of 85.44%. Isolate ACD.P4.PH9.P has a close relationship with Bacillus sp. c234 with a 98.50% similarity. Isolate ACD.P4.PH9.K has an approach with the species Bacillus sp. strain 6RM1 with a similarity of 94.78%.
Year
Volume
144
Pages
384-396
Physical description
Contributors
author
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor KM 21. 45363, West Java, Indonesia
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor KM 21. 45363, West Java, Indonesia
author
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor KM 21. 45363, West Java, Indonesia
author
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor KM 21. 45363, West Java, Indonesia
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jatinangor KM 21. 45363, West Java, Indonesia
References
  • [1] Ampou, Morrison, H.G., Huber, J.A., Mark Welch, D., Huse, S.M., Neal, P.R., Jesus, M. Arrieta. 2015. Bacteria Associations in Scleractinia Coral in Relation to the La-Nina Phenomenon on Bunaken Island. National Marine Journal, 10(02): 55-63
  • [2] Harvell, D., Jord_an-Dahlgren, E., Merkel, S., Rosenberg, E., Raymundo, L., Smith, G., Weil, E., Willis, B., 2007. Coral disease, environmental drivers, and the balance between coral and microbial associates. Oceanography 20 (1), 172-195
  • [3] Hong, M.J., Yu, Y.T., Chen, C.A., Chiang, P., Tang, S., 2009. Influence of species specificity and other factors on bacteria associated with the coral Stylophora pistillata in Taiwan. Appl Environ Microbiol. 2009 Dec; 75(24): 7797-806. doi:10.1128/AEM.01418-09
  • [4] Rizal A, Andriani Y, Kusumartono FXH, A Strategic Environmental Assessment for Southern Coastal of West Java Province, Indonesia. World Scientific News 137 (2019) 188-209.
  • [5] Rizal A. Reformulation of Regional Development Strategy To Strengthen Marine Sector in West Java, Indonesia. World Scientific News 107 (2018) 207-215
  • [6] Ritchie, K B, dan G K Smith. 1997. Physiological comparisons of bacteria from various species of Scelactinia corals. Proceedings of The 8th International Symposium for Reef Studies 521-526
  • [7] Zhang, Y., Ling, J., Yang, Q. et al. The diversity of coral associated bacteria and the environmental factors affect their community variation. Ecotoxicology 24, 1467–1477 (2015). https://doi.org/10.1007/s10646-015-1454-4
  • [8] Mulhall, M., 2009. Saving the rainforests of the sea: an analysis of international efforts to conserve coral reefs. Duke Environ. Law Policy Forum 19, 321-351
  • [9] Rosenberg, E., Koren, O., Reshef, L., Efrony, R., Zilber-Rosenberg, I., 2007. The role of microorganisms in coral health, disease and evolution. Nat. Rev. Microbiol. 5, 355-362
  • [10] Rosenberg, E., Ben-Haim, Y., 2002. Microbial diseases of corals and global warming. Environ. Microbiol. 4 (6), 318-326
  • [11] Hughes, T., Baird, A., Bellwood, D., Card, M., Connolly, S., Folke, C., Grosberg, R., Hoegh-Guldberg, O., Jackson, J.B., Kleypas, J., Lough, J.M., Marshall, P., Nystrom, M., Palumbi, S.R., Pandolfi, J.M., Rosen, B., Roughgarden, J., 2003. Climate change, human impacts, and the resilience of coral reefs. Science 301, 929-933
  • [12] Miller G., R. Beckwith, C. Fellbaum, D. Gross, K. Miller. 1990. Wordnet: An On line Lexical Database. International Journal of Lexicography, Volume 3, Issue 4, Winter 1990, Pages 235–244, https://doi.org/10.1093/ijl/3.4.235
  • [13] Claverie, Jean Michel., Notredame, Cedric. 2007 Bioinformatics for Dummies 2nd Edition. Wiley Publishing, Indianapolis, USA
  • [14] Pangastuti, A. 2006. Definition of Prokaryote Species Based on 16S rRNA and Gene Energetic Bases. Biodiversitas 7(3): 292-296
  • [15] Drancourt M., C. Bollrt, A. Carlioz, R. Martelin, J.P. Gayral, and Roult. 2000. 16S Ribosomal DNA Sequence Analysis of Enviromental and Clinical Unidentifiable Bacterial Isolates. J Clin Microbiol. 2000 Oct; 38(10): 3623-30
  • [16] Reshef, L., Koren, O., Loya, Y., Zilber-Rosenberg, I., Rosenberg, E., 2006. The coral probiotic hypothesis. Environ. Microbiol. 8, 2068-2073
  • [17] Ritchie, K.B., 2006. Regulation of microbial populations by coral surface mucus and mucus-associated bacteria. Mar. Ecol. Prog. Ser. 322, 1-14
  • [18] Jonkers, H.M., Thijssen, A., Muyzer, G., Copuroglu, O., Schlangen, E., 2010. Application of bacteria as self-healing agent for the development of sustainable concrete. J. Ecol. Eng. 36, 230–235. https://doi.org/10.1016/j.ecoleng.2008.12.036
  • [19] Lee, K.Y., Mooney, D.J., 2012. Alginate: properties and biomedical applications. J. Prog. Polym. Sci. 37, 106-126. https://doi.org/10.1016/j.progpolymsci.2011.06.003.
  • [20] Mignon, A., Snoeck, D., Halluin, K.D., Balcaen, L., Vanhaecke, F., 2016. Alginate biopolymers: counteracting the impact of superabsorbent polymers on mortar strength. J. Construct. Build. Mater. 110, 169-174. https://doi.org/10.1016/j. conbuildmat.2016.02.033
  • [21] Milla, J., Hassan, M.M., Rupnow, T., Al-Ansari, M., Arce, G., 2016. Effect of self-healing calcium nitrate microcapsules on concrete properties. J. Transport. Res. Rec. 2577, 69-77. https://doi.org/10.3141/2577-09
  • [22] Muynck, W.D., Verbeken, K., Belie, N.D., Verstraete, W., 2010a. Influence of urea and calcium dosage on the effectiveness of bacterially induced carbonate precipitation on limestone. J. Ecol. Eng. 36, 99–111. https://doi.org/10.1016/j. ecoleng.2009.03.025.
  • [23] Muynck, W.D., Belie, N.D., Verstraete, W., 2010b. Microbial carbonate precipitation in construction materials: a revie. J. Ecol. Eng. 36, 118–136. https://doi.org/10.1016/ j.ecoleng.2009.02.006
  • [24] Nguyen, T.H., Ghorbel, E., Fares, H., Cousture, A., 2019. Bacterial self-healing of concrete and durability assessment. J. Cement Concr. Compos. 104, 103340. https://doi.org/10.1016/j.cemconcomp.2019.103340
  • [25] Ouwerx, C., Velings, N., Mestdagh, M.M., Axelos, M.A.V., 1998. Physico-chemical properties and rheology of alginate gel beads formed with various divalent cations. J. Polym. Gels Netw. 6, 393-408. https://doi.org/10.1016/S0966-7822(98)00035-5
  • [26] Rohwer, F., Breitbart, M., Jara, J., Azam, F., Knowlton, N., 2001. Diversity of bacteria associated with the Caribbean coral Montastraea franksi. Coral Reefs 20, 85-91
  • [27] Rosenberg, E., Kushmaro, A., 2011. Microbial diseases of corals: pathology and ecology. In: Dubinsky, Z., Stambler, N. (Eds.), Coral Reefs: an Ecosystem in Transition. Springer, New York, pp 451-464
  • [28] Sogin, M.L., Morrison, H.G., Huber, J.A., Mark Welch, D., Huse, S.M., Neal, P.R., Jesus, M. Arrieta, Herndl, Gerhard J., 2006. Microbial diversity in the deep sea and the underexplored “rare biosphere”. Proc. Natl. Acad. Sci. U.S.A. 103, 12115-12120
  • [29] Sunagawa, S., Woodley, C.M., Medina, M., 2010. Threatened corals provide underexplored microbial habitats. PLoS One 5, e9554.
  • [30] Tang, Y.Q., Li, Y., Zhao, J.Y., Chi, C.Q., Huang, L.X., Dong, H.P., Wu, X.L., 2012. Microbial communities in long-term, water-flooded petroleum reservoirs with different in situ temperatures in the Huabei Oilfield, China. PLoS One 7, e33535
  • [31] Wahsha, M., Juhmani, A.F., Buosi, A., Sfriso, A., Sfriso, A., 2017. Assess the environmental health status of macrophyte ecosystems using an oxidative stress biomarker. Case studies: the Gulf of Aqaba and the Lagoon of Venice. Energy Procedia 125, 19-26
  • [32] Asep Sahidin, Isni Nurruhwati, Indah Riyantini, Muhamad Triandi, Structure of Plankton Communities in Cijulang River Pangandaran District, West Java Province, Indonesia. World News of Natural Sciences 23 (2019) 128-141
  • [33] Izza M. Apriliani, Zahidah Hasan, Junianto, Nadia Maudina Kusnadi, Characteristics of Gillnet Fishing Gear and Vessel Construction in Fish Landing Base of Karangsong, Indramayu, Indonesia. World News of Natural Sciences 23 (2019) 75-83
  • [34] Noir P. Purba, Yudi N. Ihsan, Ibnu Faizal, Dannisa I. W. Handyman, Kattia S. Widiastuti, Putri G. Mulyani, Mikhael F. Tefa, M. Hilmi, Distribution of Macro Debris in Savu Sea Marine National Park (Kupang, Rote, and Ndana Beaches), East Nusa Tenggara, Indonesia. World News of Natural Sciences 21 (2018) 64-76
  • [35] Wang, W., Zhong, R., Shan, D., Shao, Z., 2014. Indigenous oil-degrading bacteria in crude oil-contaminated seawater of the Yellow Sea, China. Appl. Microbiol. Biotechnol. 98, 7253–7269
  • [36] Weil, E., Smith, G., Gil, D., 2006. Status and progress in coral reef disease research. Dis. Aquat. Org. 69, 1-7
  • [37] Welsh, R.M., Zaneveld, J.R., Rosales, S.M., Payet, J.P., Burkepile, D.E., Thurber, R.V., 2016. Bacterial predation in a marine host-associated microbiome. ISME J. 10, 1540-1544
  • [38] Wilkinson, C.R., 1999. Global and local threats to coral reef functioning and existence: review and predictions. Mar. Freshw. Res. 50, 867-878
  • [39] Achmad Rizal, Zuzy Anna, Climate Change and Its Possible Food Security Implications Toward Indonesian Marine and Fisheries. World News of Natural Sciences 22 (2019) 119-128
  • [40] Achmad Rizal, Science and policy in the coastal zone management. World News of Natural Sciences 21 (2018) 1-8
Document Type
article
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.psjd-0a3d0a80-ad41-40e9-bcf0-e19be94f715a
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.