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2017 | 22 | 190 - 200
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In modern medicine, many diseases can be treated using gene therapy, which requires a DNA delivery system to prevent DNA from degradation and to transport it to the cells. Liposomal reagents are expensive for such a therapy and new inexpensive biodegradable DNA carriers are required. Chitosan (Ch) is a cationic polymer with promising potency for gene delivery. Some Ch derivatives have been shown to efficiently transfect mammalian cells in vitro. However, there are many inconsistencies in the literature concerning the effectiveness of Ch systems for in vivo gene transfer. The aim of this work was to develop a Ch-based vector for in vivo delivery of a large-size DNA fragment coding for the far-red fluorescent protein (RFP). Among several Ch derivatives, hexanoyl-Ch (HCh) with a molecular weight of 20 kDa effectively transfected cells in vitro. Intratumoural injection of polyplexes in colon and lung tumours resulted in local expression of RFP in tumours.
190 - 200
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  • Lomonosov Moscow State University, Moscow
  • Institute of Bioengineering, Research Center of Biotechnology RAS, Moscow, Russian Federation
  • Institute of Bioengineering, Research Center of Biotechnology RAS, Moscow, Russian Federation
  • Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow
  • Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow
  • Jiang HL, Cui PF, Xie RL, Cho CS; (2014) Chemical modification of chitosan for efficient gene therapy. Adv Food Nutr Res. DOI:10.1016/B978-0-12-800268-1.00006-8.
  • Fernandes JC, Qiu X, Winnik FM, Benderdour M, Zhang X, Dai K, Shi Q; (2012) Low molecular weight chitosan conjugated with folate for siRNA delivery in vitro: optimization studies. Int J Nanomedicine. DOI: 10.2147/IJN.S35567.
  • Thibault M, Nimesh S, Lavertu M, Buschmann MD; (2010) Intracellular trafficking and decondensation kinetics of chitosan-pDNA polyplexes. Mol Ther. DOI: 10.1038/mt.2010.143.
  • Köping-Höggård M, Vårum KM, Issa M, Danielsen S, Christensen BE, Stokke BT, Artursson P; (2004) Improved chitosan-mediated gene delivery based on easily dissociated chitosan polyplexes of highly defined chitosan oligomers. Gene Ther. 11(19):1441–52.
  • Layek B, Singh J; (2012) N-hexanoyl, N-octanoyl and N-decanoyl chitosans: Binding affinity, cell uptake, and transfection. Carbohydr Polym. DOI: 10.1016/j.carbpol.2012.03.021.
  • Zhu D, Jin X, Leng X, Wang H, Bao J, Liu W, Yao K, Song C; (2010) Local gene delivery via endovascular stents coated with dodecylated chitosan-plasmid DNA nanoparticles. Int J Nanomedicine. DOI: 10.2147/IJN.S14358.
  • Du YZ, Lu P, Yuan H, Zhou JP, Hu FQ; (2011) Quaternary complexes composed of plasmid DNA/protamine/fish sperm DNA/stearic acid grafted chitosan oligosaccharide micelles for gene delivery. Int J Biol Macromol. DOI: 10.1016/j.ijbiomac.2010.10.011.
  • Hu FQ, Zhao MD, Yuan H, You J, Du YZ, Zeng S; (2006) A novel chitosan oligosaccharide-stearic acid micelles for gene delivery: properties and in vitro transfection studies. Int J Pharm. 315(1–2):158–66.
  • Wang B, He C, Tang C, Yin C; (2011) Effects of hydrophobic and hydrophilic modifications on gene delivery of amphiphilic chitosan based nanocarriers. Biomaterials. DOI: 10.1016/j.biomaterials.2011.03.003.
  • Gaur S, Wen Y, Song JH, Parikh NU, Mangala LS, Blessing AM, Ivan C, Wu SY, Varkaris A, Shi Y, Lopez-Berestein G, Frigo DE, Sood AK, Gallick GE; (2015) Chitosan nanoparticle-mediated delivery of miRNA-34a decreases prostate tumor growth in the bone and its expression induces non-canonical autophagy. Oncotarget. DOI: 10.18632/oncotarget.4971.
  • Kim MJ, Park JS, Lee SJ, Jang J, Park JS, Back SH, Bahn G, Park JH, Kang YM, Kim SH, Kwon IC, Jo DG, Kim K; (2015) Notch1 targeting siRNA delivery nanoparticles for rheumatoid arthritis therapy. J Control Release. DOI: 10.1016/j.jconrel.2015.08.025.
  • Oliveira AV, Silva GA, Chung DC; (2015) Enhancement of chitosan-mediated gene delivery through combination with phiC31 integrase. Acta Biomater. DOI: 10.1016/j.actbio.2015.01.013.
  • Sankov V, Shagdarova B, Chudakov D, Reshetov P, Grechikhina M, Zubareva A, Varlamov V, Esipov R, Zubov V, Svirshchevskaya E; (2017) Synthesis and evaluation of chitosan derivatives or large size DNA delivery. Org Medicinal Chem IJ. (In press).
  • Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM; (2007) Bright far-red fluorescent protein for whole-body imaging. Nat Methods. 4(9):741–6.
  • Shagdarova BTs., Il’ina AV., Varlamov VP; (2016) Antibacterial activity of alkylated and acylated derivatives of low-molecular weight chitosan. Appl Biochem Microbiol. 52(2):222–5.
  • Lin XY, Chen SZ; (2017) Calpain inhibitors ameliorate muscle wasting in a cachectic mouse model bearing CT26 colorectal adenocarcinoma. Oncol Rep. DOI: 10.3892/or.2017.5396.
  • Park BY, Min BS, Ahn KS, Kwon OK, Joung H, Bae KH, Lee HK, Oh SR; (2007) Daphnane diterpene esters isolated from flower buds of Daphne genkwa induce apoptosis in human myelocytic HL-60 cells and suppress tumor growth in Lewis lung carcinoma (LLC)-inoculated mouse model. J Ethnopharmacol. 111(3):496–503.
  • Buschmann MD, Merzouki A, Lavertu M, Thibault M, Jean M, Darras V; (2013) Chitosans for delivery of nucleic acids. Adv Drug Deliv Rev. doi: 10.1016/j.addr.2013.07.005.
  • Welsh, S., S.A. Kay; (1997) Reporter gene expression for monitoring gene transfer. Curr Opin Biotechnol. 8:617–622.
  • Yan C, Jie L, Yongqi W, Weiming X, Juqun X, Yanbing D, Li Q, Xingyuan P, Mingchun J, Weijuan G; (2015) Delivery of human NKG2D-IL-15 fusion gene by chitosan nanoparticles to enhance antitumor immunity. Biochem Biophys Res Commun. DOI: 10.1016/j.bbrc.2015.05.065.
  • Lyalina T, Zubareva A, Lopatin S, Zubov V, Sizova S, Svirshchevskaya E; (2017) Correlation analysis of chitosan physicochemical parameters determined by different methods. Org Medicinal Chem IJ. DOI: 10.19080/OMCIJ.2017.01.555562.
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