NEW AFFINITY SORBENTS FOR PURIFICATION OF RECOMBINANT PROTEINS WITH THE USE OF CHITIN-BINDING DOMAIN AS AN AFFINITY TAG

• Authors: Denis V. Kurek , Sergey A. Lopatin , Vladimir E. Tikhonov , Valery P. Varlamov
• Languages of publication: EN

Abstracts

EN

With the increasing amounts of recombinant proteins production the problem of effective purification of produced proteins became one of major topics for research nowadays. Affinity chromatography proved to be the most dominant method for this aim and application of chitin based affinity sorbents in combination with use of chitin-binding domain as an affinity tag for purification of recombinant proteins showed its efficiency even in comparison with commercially available systems.

Keywords

EN

affinity chromatography; chitin based sorbents; chitin-binding domain

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2010
• Volume: 15
• Pages: 41 - 46

Contributors

author

Denis V. Kurek

• A. N. Nesmeyanov Institute of Organoelement Compounds RAS,

author

Sergey A. Lopatin

• A. N. Nesmeyanov Institute of Organoelement Compounds RAS,

author

Vladimir E. Tikhonov

• A. N. Nesmeyanov Institute of Organoelement Compounds RAS,

author

Valery P. Varlamov

• A. N. Nesmeyanov Institute of Organoelement Compounds RAS,

References

• Graslund S., Nordlund P., Weigelt J., at al.; (2008), Nature Methods. V. 5. № 2. P. pp. 135-145.
• Kurek D.V., Lopatin S. A., Varlamov V. P.; (2009), Appl. Biochem. Microbiol. V. 45, №1, P. pp. 5-13.
• Redo V.A., Eldarov M.A., Zhgoun А.А., Khatuntseva S.A., Zeinalov O.A., Skryabin K.G.; RF patent № 2310688, 20.11.2007.
• Hardt M., Laine R.; Arch. (2004), Biochem. Biophys. V. 426. P. pp. 286-297.

• Document Type: article