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2010 | 15 | 41 - 46
Article title

NEW AFFINITY SORBENTS FOR PURIFICATION OF RECOMBINANT PROTEINS WITH THE USE OF CHITIN-BINDING DOMAIN AS AN AFFINITY TAG

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EN
Abstracts
EN
With the increasing amounts of recombinant proteins production the problem of effective purification of produced proteins became one of major topics for research nowadays. Affinity chromatography proved to be the most dominant method for this aim and application of chitin based affinity sorbents in combination with use of chitin-binding domain as an affinity tag for purification of recombinant proteins showed its efficiency even in comparison with commercially available systems.
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Year
Volume
15
Pages
41 - 46
Physical description
Contributors
  • A. N. Nesmeyanov Institute of Organoelement Compounds RAS,
  • A. N. Nesmeyanov Institute of Organoelement Compounds RAS,
  • A. N. Nesmeyanov Institute of Organoelement Compounds RAS,
References
  • Graslund S., Nordlund P., Weigelt J., at al.; (2008), Nature Methods. V. 5. № 2. P. pp. 135-145.
  • Kurek D.V., Lopatin S. A., Varlamov V. P.; (2009), Appl. Biochem. Microbiol. V. 45, №1, P. pp. 5-13.
  • Redo V.A., Eldarov M.A., Zhgoun А.А., Khatuntseva S.A., Zeinalov O.A., Skryabin K.G.; RF patent № 2310688, 20.11.2007.
  • Hardt M., Laine R.; Arch. (2004), Biochem. Biophys. V. 426. P. pp. 286-297.
Document Type
article
Publication order reference
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YADDA identifier
bwmeta1.element.psjd-0518dde5-4714-421b-bd62-742f1aab345d
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