Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl

PL EN


Preferences help
enabled [disable] Abstract
Number of results
2011 | 50 | 3 |

Article title

Application of a Multiplex PCR with Specific PCR Primers for the Detection of the Genus Paramecium and the Paramecium aurelia Complex

Content

Title variants

Languages of publication

PL

Abstracts

PL
The representatives of the genus Paramecium are well-studied ciliates and can be used in water quality assessment and the determinations of saprobic levels. For these applications, a clear and unambiguous identification of ciliate assemblages is essential, which is typically based on morphological characters requiring a sound taxonomic knowledge and experience in species determination including microscopic identification of both living and stained specimens. Therefore, we developed and applied specific PCR primers for the detection of species belonging to the genus Paramecium and the Paramecium aurelia complex. These primers were successfully tested with different Paramecium species including representatives of the P. aurelia complex as well as closely related species like Frontonia sp. and Tetrahymena sp. in both experimental and environmental samples. These primers can be used in a simultaneous approach achieving fast and reliable results with regard to determination of ciliate community and water assessment.

Year

Volume

50

Issue

3

Physical description

Dates

published
2011
online
27 - 08 - 2015

Contributors

References

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.ojs-issn-1689-0027-year-2011-volume-50-issue-3-article-3918
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.