The Effects of Linoleic Acid on the Fermentation Parameters, Population Density, and Fatty-acid Profile of Two Rumen Ciliate Cultures, Entodinium caudatum and Diploplastron affine

• Authors: Adam CIEŚLAK , Svetlana KIŠIDAYOVÁ , Małgorzata SZUMACHERSTRABEL
• Languages of publication: PL

Abstracts

PL

The effects of linoleic acid supplementation on protozoa counts, fermentation parameters and fatty-acid composition of Entodinium caudatum and Diploplastron affine were examined in in vitro cultures. Entodinium caudatum (EC) and Diploplastron affine (DA) were isolated from the rumen of sheep (Slovak Merino breed) and cultivated anaerobically in the presence of bacteria in caudatum-type medium. To test the effect of soluble linoleic acid (LA) on protozoan growth, both ciliate species were supplemented with LA (1.51 μg/L) on the day of dilution over a 30-day period. Twenty-four-hour fermentation parameters were examined on both ciliate cultures and their respective bacterial fractions. Ciliate counts of both EC and DA cultures were not significantly affected by supplemented LA. The major impact of the soluble form of LA supplement was found in the bacterial fractions of both ciliate cultures. LA supplementation had a greater effect on fermentation parameters and fatty-acid contents in the EC experimental groups than in the DA groups. Our results suggest that experimental rumen ciliates and their associated bacterial populations had different metabolic responses to the tested form and concentration of supplemented LA.

Keywords

PL

Protozoa, fatty acids, rumen fermentation parameters, in vitro

• Publisher: Uniwersytet Jagielloński
• Journal: Acta Protozoologica
• Year: 2009
• Volume: 48
• Issue: 1

Dates

published

2009

online

25 - 09 - 2015

Contributors

author

Adam CIEŚLAK

author

Svetlana KIŠIDAYOVÁ

author

Małgorzata SZUMACHERSTRABEL