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2022 | 61 | 35-46

Article title

Future prospects for investigating ciliate biodiversity

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Abstracts

EN
Ciliates have a long history of being central in evolutionary and ecological studies on eukaryotic microorganisms. Although thousands of species have been discovered, their total diversity still remains unknown. Here, we will discuss two unsolved problems that hinder the further exploration of ciliate diversity at the species level, and potential solutions to these problems are proposed. First, ciliate morphospecies are difficult to identify because the different silver stains are not scalable (they do not represent high-throughput methods) and basic supplies are lacking (e.g., protargol); a solution may be the development of fluorescent staining techniques. Second, ciliate phylogenetic species are difficult to identify because of extensive paralogy in nuclear-protein-coding genes; a solution may be to concentrate on sequencing mitochondrial genomes. These two approaches could be integrated into a high-throughput fluorescent-single-cell sorting and mitochondrial genomes sequencing process that would enable the observation and better understanding of ciliate species on a massive scale.

Year

Volume

61

Pages

35-46

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Dates

published
2022

Contributors

  • Phycology, Faculty of Biology, University of Duisburg-Essen, Essen, Germany
  • Eukaryotic Microbiology, Faculty of Biology, University of Duisburg-Essen, Essen, Germany
author
  • Institute of Evolution and Marine Biodiversity & College of Fisheries, Ocean University of China, Qingdao, China
  • Eukaryotic Microbiology, Faculty of Biology, University of Duisburg-Essen, Essen, Germany
  • Eukaryotic Microbiology, Faculty of Biology, University of Duisburg-Essen, Essen, Germany
  • Natural History Museum, University of Oslo, Oslo, Norway

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Document Type

Publication order reference

Identifiers

Biblioteka Nauki
52000785

YADDA identifier

bwmeta1.element.ojs-doi-10_4467_16890027AP_22_005_16236
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