Antifreeze Water-Rich Dormant Cysts of the Terrestrial Ciliate Colpoda cucullus Nag-1 at −65 ℃: Possible Involvement of Ultra-Antifreeze Polysaccharides

• Authors: Tatsuomi Matsuoka , Yoichiro Sogame , Rikiya Nakamura , Yuya Hasegawa , Mikihiko Arikawa , Futoshi Suizu

Abstracts

EN

We found that the water-rich (osmolality below 0.052 Osm/l) wet resting cysts of the soil ciliate Colpoda cucullus Nag-1 were tolerant to extremely low temperature (−65℃). When cell fluid obtained from the resting cysts was cooled at −65℃, small particles of ice crystals did not grow into large ice crystals. At −65℃, the cysts shrank due to an outflow of water, because a vapor pressure difference was produced between the cell interior and freezing surrounding medium. The osmolality of these shrunk cells was estimated 0.55 Osm/l, and the freezing point depression of the shrunk cell fluid was estimated to be 1.02℃. Hence, the antifreeze ability of wet cysts at −65℃can not be explained by freezing point depression due to elevation of cytoplasmic osmolality. The cytoplasm of resting cysts was vividly stained red with periodic acid-Schiff (PAS) and stained purple with toluidine blue. On the other hand, the excystment-induced cysts were not stained with PAS, and exhibited a loss of the antifreeze activity. PAS staining of SDSPAGE gel obtained from encysting Colpoda cells showed that a large amount of PAS-positive macromolecules accumulated as the encystment stage progressed. These results suggest that antifreeze polysaccharides may be involved in the antifreeze activity of C. cucullus Nag-1 dormant forms.

Keywords

EN

water-rich resting cyst; antifreeze; polysaccharides; osmolarity

• Publisher: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
• Journal: Acta Protozoologica
• Year: 2020
• Volume: 59
• Issue: 3-4
• Pages: 141-147

Dates

published

2020

Contributors

author

Tatsuomi Matsuoka

• Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan

author

Yoichiro Sogame

• Department of Applied Chemistry & Biochemistry, National Institute of Technology, Fukushima College
• Department of Biological Science, Faculty of Science, Kochi University

author

Rikiya Nakamura

• Department of Biological Science, Faculty of Science, Kochi University
• Chikazawa Paper Co., Ltd., Japan

author

Yuya Hasegawa

• Department of Biological Science, Faculty of Science, Kochi University

author

Mikihiko Arikawa

• Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan

author

Futoshi Suizu

• Division of Cancer Biology, Institute for Genetic Medicine, Hokkaido University

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Identifiers

DOI

10.4467/16890027AP.20.011.13266

Biblioteka Nauki

52089832