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2020 | 59 | 3-4 | 141-147

Article title

Antifreeze Water-Rich Dormant Cysts of the Terrestrial Ciliate Colpoda cucullus Nag-1 at −65 ℃: Possible Involvement of Ultra-Antifreeze Polysaccharides

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Abstracts

EN
We found that the water-rich (osmolality below 0.052 Osm/l) wet resting cysts of the soil ciliate Colpoda cucullus Nag-1 were tolerant to extremely low temperature (−65℃). When cell fluid obtained from the resting cysts was cooled at −65℃, small particles of ice crystals did not grow into large ice crystals. At −65℃, the cysts shrank due to an outflow of water, because a vapor pressure difference was produced between the cell interior and freezing surrounding medium. The osmolality of these shrunk cells was estimated 0.55 Osm/l, and the freezing point depression of the shrunk cell fluid was estimated to be 1.02℃. Hence, the antifreeze ability of wet cysts at −65℃can not be explained by freezing point depression due to elevation of cytoplasmic osmolality. The cytoplasm of resting cysts was vividly stained red with periodic acid-Schiff (PAS) and stained purple with toluidine blue. On the other hand, the excystment-induced cysts were not stained with PAS, and exhibited a loss of the antifreeze activity. PAS staining of SDSPAGE gel obtained from encysting Colpoda cells showed that a large amount of PAS-positive macromolecules accumulated as the encystment stage progressed. These results suggest that antifreeze polysaccharides may be involved in the antifreeze activity of C. cucullus Nag-1 dormant forms.

Year

Volume

59

Issue

3-4

Pages

141-147

Physical description

Dates

published
2020

Contributors

  • Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
  • Department of Applied Chemistry & Biochemistry, National Institute of Technology, Fukushima College
  • Department of Biological Science, Faculty of Science, Kochi University
  • Department of Biological Science, Faculty of Science, Kochi University
  • Chikazawa Paper Co., Ltd., Japan
author
  • Department of Biological Science, Faculty of Science, Kochi University
  • Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
author
  • Division of Cancer Biology, Institute for Genetic Medicine, Hokkaido University

References

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Document Type

Publication order reference

Identifiers

Biblioteka Nauki
52089832

YADDA identifier

bwmeta1.element.ojs-doi-10_4467_16890027AP_20_011_13266
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