A New Trichodina Species (Peritrichia: Mobilida) from Anuran Tadpole Hosts, Sclerophrys spp. in the Okavango Panhandle, Botswana, with Comments on this Taxon

• Authors: Gerhard P. de Jager , Linda Basson , Jacqueline van Marwijk

Abstracts

EN

Mobiline taxonomic data is mostly inferred from populations collected in and on hosts associated with aquaculture. Even though these conditions may be conducive for studies relating to the hosts, accurate taxonomic inferences of the symbionts will be problematic. The site for the present study was the Okavango Panhandle region in Northern Botswana, an isolated, natural area with minimal anthropogenic influences. Morphometric and molecular evidence revealed that anuran tadpole trichodinids, up to now reported as Trichodina heterodentata Duncan 1977 and T. hypsilepis Wellborn 1967 from multiple host types, are in fact a new, more host specific species. This study includes comprehensive denticle descriptions of both the anuran hosted trichodinid and the morphologically similar T. hypsilepis restricted to teleost hosts (previously T. heterodentata).

Keywords

EN

Trichodina koloti sp. nov.; Sclerophrys spp.; morphology; 18S ribosomal DNA; southern Africa

• Publisher: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
• Journal: Acta Protozoologica
• Year: 2019
• Volume: 58
• Issue: 3
• Pages: 141-153

Dates

published

2019

Contributors

author

Gerhard P. de Jager

• Department of Zoology and Entomology, University of the Free State, Bloemfontein, South Africa

author

Linda Basson

• Department of Zoology and Entomology, University of the Free State, Bloemfontein, South Africa

author

Jacqueline van Marwijk

• Department of Microbial, Biochemical, and Food Biotechnology, University of the Free State, Bloemfontein, South Africa

References

• Abdelkhalek N. K., Mohamed A., Salama M. F., Elmishmishy B., Ali M. O., El-Ashram A., Hamed M. F., Al-Araby M. A. (2018) Molecular identification of Trichodina compacta van As and Basson, 1989 (Ciliophora: Peritrichia) from cultured Oreochromis niloticus in Egypt and its impact on immune responses and tissue pathology. Parasitol. Res. 117: 1907–1914
• Arthur J. R., Lom J. (1984) Some trichodinid ciliates (Protozoa: Peritrichida) from Cuban fishes, with a description of Trichodina cubanensis n. sp. from the skin of Cichlasoma tetracantha. T. Am. Microsc. Soc. 103: 172–184
• Basson L., van As J. G., Paperna I. (1983) Trichodinid ectoparasites of cichlid and cyprinid fishes in South Africa and Israel. Syst. Parasitol. 5: 245–257
• Channing A., Rödel M. O., Channing J. (2012). Tadpoles of Africa: The biology and identification of all known tadpoles in sub-Saharan Africa. Chimaira Buchhandelgesellschaft, Frankfurt am Main
• Chen C. L. (1963) Studies on the ectoparasitic trichodinids from freshwater fish, tadpole and crustacean. Acta Hydrobiol. Sin. 3: 99–111
• Clark K., Karsch-Mizrachi I., Lipman D. J., Ostell J., Sayers E. W. (2016) GenBank. Nucleic Acids Res. 44(D1):D67–72
• de Jager G. P., Basson L. (2019) Taxonomic assessment of three North American trichodinids by re-evaluating the taxon validity of Trichodina heterodentata Duncan, 1977 (Peritrichia). Acta Protozool. 58: 125–139
• Dias R. J. P., Fernandes N. M., Sartini B., da Silva-Neto I. D., D’Agosto M. (2009) Occurrence of Trichodina heterodentata (Ciliophora: Trichodinidae) infesting tadpoles of Rhinella pombali (Anura: Bufonidae) in the Neotropical area. Parasitol. Int. 58: 471–474
• Duncan B. L. (1977) Urceolariid ciliates, including three new species, from cultured Philippine fishes. Trans. Am. Microsc. Soc. 96: 79–81
• du Preez L., Carruthers V. C. (2009) A complete guide to the frogs of southern Africa. Struik Nature, Cape Town Fernandes N. M., Sartini B., Dias R. J. P., D’Agosto M. (2011) Quantitative study of Trichodina heterodentata (Ciliophora: Mobilia) infrapopulations infesting tadpoles of a Brazilian endemic toad Rhinella pombali (Anura: Bufonidae). Zoologia-Curitiba 28: 777–783
• Felsenstein J. (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791
• Gong Y. C., Yu Y. H., Villalobo E., Zhu F. Y., Miao, W. (2006) Reevaluation of the phylogenetic relationship between mobilid and sessilid peritrichs (Ciliophora, Oligohymenophorea) based on small subunit rRNA genes sequences. J. Eukaryot. Microbiol. 53: 397–403
• Irwin N. A. T., Lynn D. H. (2015) Molecular phylogeny of mobilid and sessilid ciliates symbiotic in eastern Pacific limpets (Mollusca: Patellogastropoda). J. Eukaryot. Microbiol. 62: 543–552
• Irwin N. A. T., Sabetrasekh M., Lynn D. H. (2017). Diversification and phylogenetics of mobilid peritrichs (Ciliophora) withdescription of Urceolaria parakorschelti sp. nov. Protist 168: 481–493
• Kazubski S. L. (1988) Morphological variation in a ciliate, Trichodina reticulata Hirschmann et Partsch, 1955 (Peritrichida), in tadpoles from small ponds. Acta Protozool. 27: 259–269
• Kimura M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Mol. Biol. Evol. 16: 111–120
• Klein B. M. (1926) Die Silberliniensysteme der Ciliaten. ihr Verhaken wahrend Teilung und Conjugation. Arch. Protistenkd. 58: 55–142
• Kruger J., van As J. G., Basson L. (1993a) Trichodina heterodentata Duncan, 1977 (Ciliophora: Peritrichida), an ectoparasite on larvae of the African Clawed toad Xenopus laevis laevis (Daudin, 1802). Acta Protozool. 32: 255–259
• Kruger J., Basson L., van As J.G. (1993b) On the ultrastructure of the adhesive disc of Trichodina xenopodos Fantham, 1924 and T. heterodentata Duncan, 1977 (Ciliophora: Peritrichida). Acta Protozool. 32: 245–253
• Kruger J., van As J. G., Basson L. (1995) Observations on the adhesive disc of Trichodina xenopodos, Fantham, 1924 and T. heterodentata Duncan, 1977 (Ciliophora: Peritrichida) during binary fission. Acta Protozool. 34: 203–209
• Kumar S., Stecher G., Li M., Knyaz C., Tamura K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol. Biol. Evol. 35: 1547–1549
• Lom J. (1958) A contribution to the systematics and morphology of endoparasitic trichodinids from amphibians, with a proposal of uniform specific characteristics. J. Protozool. 5: 251–263
• Lom J. (1961) Ectoparasitic trichodinids from fresh water fish in Czechoslovakia. Acta. Soc. Zool. Bohemoslov. 25: 215–228
• Lynn D. H. (2017). Ciliophora. In: Handbook of the Protists (Eds. J. M. Archibald, A. G. B. Simpson, C. H. Slamovits). Springer, Cham, 679–730
• Marcotegui P. S., Montes M. M., Barneche J., Ferrari W., Martorelli, S. (2018) Geometric morphometric on a new species of Trichodinidae. A tool to discriminate trichodinid species combined with traditional morphology and molecular analysis. Int. J. Parasitol. Parasites Wildl. 7: 228–236
• Medlin L., Elwood H. J. Sticker S., Sogin M. L. (1988) The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71: 491–499
• Nei M., Kumar S. (2000). Molecular evolution and phylogenetics. Oxford University Press, New York
• Pádua S. B., Martins M. L., Carraschi S. P., Cruz C., Ishikawa M. (2012) Trichodina heterodentata (Ciliophora: Trichodinidae): a new parasite for Piaractus mesopotamicus (Pisces: Characidae). Zootaxa 3422: 62–68
• Pala G., Valladão G. M. R., Alves L. O., Pilarski F., Hopp E. L. (2018) Tadpoles of Rhinella schneideri as reservoirs of trichodinids in continental aquaculture. Aquaculture 488: 17–21
• Raabe Z. (1950) Remarques sur les Urceolariides (Ciliata – Peritricha) des branches des poissons. Ann. Univ. Mariae Curie-Skłodowska 5: 291–310
• Ronquist F., Huelsenback J. P. (2003) Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574
• Saitou N., Nei M. (1987) The Neighbour-Joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–425
• Sogin M. L., Silberman J. D. (1998) Evolution of the protists and protistan parasites from the perspective of molecular systematics. Int. J. Parasitol. 28: 11–20
• Tang F. H., Zhao Y. J. (2007) Taxonomic studies of three species of Trichodina Ehrenberg, 1838 with pathologic research into gill tissue of Carassius auratus caused by Trichodina heterodentata Duncan, 1977: a study of trichodinids from freshwater fishes in Chongqing II. J. Chongqing Norm. Univ. Nat. Sci. Ed. 24: 8
• Tang F. H., Zhao Y. J. (2016) Molecular phylogenetic evidences on Mobilida based on genetic distance and GC content of 18S rDNA using broad taxon sampling. Acta Hydrobiol. 4: 358–369
• Tang F. H., Zhao Y. J., Warren A. (2013) Phylogenetic analyses of trichodinids (Ciliophora, Oligohymenophorea) inferred from 18S rRNA gene sequence data. Curr. Microbiol. 66: 306–313
• Tang F., Zhang Y., Zhao Y. (2017) Morphological and molecular identification of the new species, Trichodina pseudoheterodentata sp. n. (Ciliophora, Mobilida, Trichodinidae) from the channel catfish, Ictalurus punctatus, in Chongqing China. J. Eukaryot. Microbiol. 64: 45–55
• Valladão G. M. R., Gallani S. U., de Pádua S. B., Martins M. L., Pilarski F. (2013) Trichodina heterodentata (Ciliophora) infestation on Prochilodus linaetus larvae: A host–parasite relationship study. Parasitology 141: 662–669
• van As J. G., Basson L. (1989) A further contribution to the taxonomy of the Trichodinidae (Ciliophora: Peritrichia) and a review of the taxonomic status of some fish ectoparasitic trichodinids. Syst. Parasitol. 14: 157–179
• van As J. G., Basson L. (1992) Trichodinid ectoparasites (Ciliophora: Peritrichida) of freshwater fishes of the Zambesi River System, with a reappraisal of host specificity. Syst. Parasitol. 22: 81–109
• Wang Q., Tang F., Zhao Y-J. (2015) Clone and sequence analysis of 18S rDNA of Trichodina centrostrigata. J. Chongqing Norm. Univ. Nat. Sci. Ed. 32: 31–37
• Wang Z., Zhou T., Gu Z. (2017) New data of two trichodinid ectoparasites (Ciliophora: Trichodinidae) from farmed freshwater fishes in Hubei, China. Eur. J. Protistol. 60: 50–59
• Wang Z., Deng Q., Zhou T., Yang H., Gu, Z. (2018) First record of two ectoparasitic ciliates of the genus Trichodina (Ciliophora: Trichodinidae) parasitizing gills of an invasive freshwater fish, Micropercops swinhonis, in Tibet. Parasitol. Res.117: 2233–2242
• Wellborn T. L. (1967) Trichodina (Ciliata: Urceolariidae) of freshwater fishes of the South-eastern United States. J. Protozool. 14: 399–412
• West D. T., van As J. G., van As L. L. (2015) Surface water quality in the Okavango Delta panhandle, Botswana. Afr. J. Aquat. Sci. 40: 359–372
• Wiroonpan P., Purivirojkul W. (2019) New record of Trichodina unionis (Ciliophora, Trichodinidae) from freshwater gastropods in Bangkok, Thailand. Parasite. 26: 1–10
• Zhan Z., Xu K., Warren A., Gong, Y. (2009) Reconsideration of phylogenetic relationships of the subclass Peritrichia (Ciliophora, Oligohymenophorea) based on small subunit ribosomal RNA gene sequences, with the establishment of a new subclass Mobilia Kahl, 1933. J. Eukaryot. Microbiol. 56: 552–558
• Zhan Z., Xu K., Dunthorn M. (2013) Evaluating molecular support for and against the monophyly of the Peritrichia and phylogenetic relationships within the Mobilida (Ciliophora, Oligohymenophorea). Zool. Scr. 42: 213–226

Identifiers

DOI

10.4467/16890027AP.19.014.11915

Biblioteka Nauki

52089989