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2018 | 57 | 1 | 43-48

Article title

New Primers for Fast Detection of Giardia duodenalis Assemblages A and B Using Real-time PCR

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Abstracts

EN
Giardia duodenalisis one of the six Giardia species and itis the most common, cosmopolitan flagellate that infects humans and many species of animals. This species exhibits considerable genetic diversity; to date, eight assemblages (A–H) have been defined. These assemblages differ in host specificity: assemblages A and B have been found in both humans and in many animal species. Mixed infections with Giardia (A and B) assemblages have been reported in humans and in animals. Many molecular techniques are effective and rapid for the detection of G. duodenalis and also for the determination of genetic variability of isolates in clinical and environmental samples. In this context, the aim of this study was to design new assemblage-specific primers for rapid detection and identification of G. duodenalis assemblages A and B and both of these assemblages simultaneously using quantitative real-time polymerase chain reaction (qPCR). Fragments of glutamate dehydrogenase and triose phosphate isomerase were used as targets in the design of primers. In conclusion, the use of G. duodenalis assemblage-specific primers designed in this study allows quick identification of human infectious G. duodenalis assemblages A and B as well as mixed AB assemblages in a sample without further sequencing of the amplification products, which reduces the cost of study and the waiting time for the results.

Year

Volume

57

Issue

1

Pages

43-48

Physical description

Dates

published
2018

Contributors

  • Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Poznan, Poland
  • Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Poznan, Poland
  • Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland
  • Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Poznan, Poland
  • Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland
  • Department of Parasitology, National Veterinary Research Institute, Pulawy, Poland
author
  • The Witold Stefański Institute of Parasitology, Polish Academy of Sciences, Warszawa, Poland
  • Poznan University of Medical Sciences, Poznan, Poland
  • Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Poznan, Poland

References

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Document Type

Publication order reference

Identifiers

Biblioteka Nauki
52160005

YADDA identifier

bwmeta1.element.ojs-doi-10_4467_16890027AP_18_003_8397
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