Allovahlkampfia minuta nov. sp., (Acrasidae, Heterolobosea, Excavata) a New Soil Amoeba at the Boundary of the Acrasid Cellular Slime Moulds

• Authors: Alvaro De Obeso Fernadez Del Valle , Sutherland K. Maciver

Abstracts

EN

We report the isolation of a new species of Allovahlkampfia, a small cyst-forming heterolobosean soil amoeba. Phylogenetic analysis of the 18S rDNA and the internal transcribed spacers indicates that Allovahlkampfia is more closely related to the acrasids than to other heterolobosean groups and indicates that the new strain (GF1) groups with Allovahlkampfia tibetiensis and A. nederlandiensis despite being significantly smaller than these and any other described Allovahlkampfia species. GF1 forms aggregated cyst masses similar to the early stages of Acrasis sorocarp development, in agreement with the view that it shares ancestry with the acrasids. Time-lapse video microscopy reveals that trophozoites are attracted to individuals that have already begun to encyst or that have formed cysts. Although some members of the genus are known to be pathogenic the strain GF1 does not grow above 28oC nor at elevated osmotic conditions, indicating that it is unlikely to be a pathogen. 

• Publisher: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
• Journal: Acta Protozoologica
• Year: 2017
• Volume: 56
• Issue: 3
• Pages: 181-189

Dates

published

2017

Contributors

author

Alvaro De Obeso Fernadez Del Valle

• Biomedical Sciences, Edinburgh Medical School, University of Edinburgh, Scotland, UK

author

Sutherland K. Maciver

• Biomedical Sciences, Edinburgh Medical School, University of Edinburgh, Scotland, UK

References

• Aitken D., Hay J., Kinnear F. B., Kirkness C. M., Lee W. R., Seal D.V. (1996) Amebic keratitis in a wearer of disposable contact lenses due to a mixed Vahlkampfia and Hartmanella infection. Ophthalmol.103: 485–494
• Anderson O. R., Wang W. E. N., Faucher S. P., Bi K., Shuman H. A. (2011) A new heterolobosean amoeba Solumitrus palustris n. g., n. sp. isolated from freshwater marsh soil. J. Eukaryot. Microbiol. 58: 60–67
• Alexandrakis G., Miller D., Huang A. J. W. (1998) Amebic keratitis due to Vahlkampfia infection following corneal trauma. Arch. Ophthalmol. 117: 950–951
• Brown S., De Jonckheere J. F. (1999) A reevaluation of the amoeba genus Vahlkampfia based on SSUrDNA sequences. Eur. J. Protistol. 35: 49–54
• Brown M. W., Silberman J. D., Spiegel F. W. (2010) A morphologically simple species of Acrasis (Heterolobosea, Excavata), Acrasis helenhemmesae n. sp. J. Eukaryot. Microbiol. 57: 346–353
• Brown M. W., Silberman J. D., Spiegel F. W. (2012) A contemporary evaluation of the acrasids (Acrasidae, Heterolobosea, Excavata). Eur. J. Protistol. 48: 103–123
• Carter R. F. (1970) Description of a Naegleria species isolated from two cases of primary amoebic meningoencephalitis and of the experimental pathological changes induced by it. J. Pathol. 100: 217–244
• De Jonckheere J. F., Murase J., Opperdoes F. R. (2011) A new thermophilic heterolobosean amoeba, Fumarolamoeba ceborucoi, gen. nov., sp. nov., isolated near a fumarole at a volcano in Mexico. Acta Protozool. 50: 41–48
• Droop M. R. (1962) Heteramoeba clara n. gen., n. sp., a sexual biphasic amoeba. Arch. Microbiol. 42: 254–266
• Garstecki T., Brown S., De Jonckheere J. F. (2005) Description of Vahlkampfia signyensis n. sp. (Heterolobosea), based on morphological, ultrastructural and molecular characteristics. Eur. J. Protistol. 41: 119–127
• Geisen S., Bonkowski M., Zhang J., De Jonckheere J. F. (2015) Heterogeneity in the genus Allovahlkampfia and the description of the new genus Parafumarolamoeba (Vahlkampfiidae; Heterolobosea). Eur. J. Protistol. 51: 335–349
• González-Robles A., Salazar-Villatoro L., González-Lázaro M., Omaña-Molina M., Martínez-Palomo A. (2012) Vahlkampfia sp: Structural observations of cultured trophozoites. Exp. Parasitol. 130: 86–90
• Gouy M., Guindon S., Gascuel O. (2010) SeaView Version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol. Biol. Evol. 27: 221–224
• Guindon S., Gascuel O., (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 52: 696–704
• Hall T. A., (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41: 95–98
• Harding T., Brown M. W., Plotnikov A., Selivanova E., Park J. S., Gunderson J. H., Baumgartner M., Silberman J. D., Roger A. J., Simpson A. G. (2013) Amoeba stages in the deepest branching heteroloboseans, including Pharyngomonas: evolutionary and systematic implications. Protist 164: 272–286
• Hohl H. R., Hamamoto S. T. (1969) Ultrastructure of Acrasis rosea, a cellular slime mold, during development. J. Protozool. 16: 333–344
• Huseein E. A. M., Anwer F. E. S., Abed G. H., Abdel-Gahfar K. S., Omar H. E. D. M. (2016) Keratitis induced by Allovahlkampfia spelaea in experimental rat model: a trial for treatment with ellagic acid. Eur. J. Biol. Res. 6: 299–309
• Kang S., Tice A. K., Spiegel F. W., Silberman J. D., Pánek T., Čepička I., Kostka M., Kosakyan A., Alcântara D. M., Roger A. J., Shadwick L. L., Smirnov A., Kudryavstev A., Lahr D. J. G., Brown M. W. (2017) Between a pod and a hard test: the deep evolution of amoebae. Mol. Biol.Evol. 34: 2258–2270
• Keeling P. J., Doolittle W. F. (1996) Alpha-tubulin from early diverging eukaryotic lineages and the evolution of the tubulin family. Mol. Biol. Evol. 13: 1297–1305
• Khan N. A., Jarroll E. J., Paget T. A. (2001) Acanthamoeba can be differentiated by the polymerase chain reaction and simple plating assays. Curr. Microbiol. 43: 204–208
• Lara E., Berney C., Ekelund F., Harms H., Chatzinotas A. (2007) Molecular comparison of cultivable protozoa from a pristine and a polycyclic aromatic hydrocarbon polluted site. Soil Biol.Biochem. 39: 139–148
• Lorenzo-Morales J., Lindo J. F., Martinez E., Calder D., Figueruelo E., Valladares B., Ortega-Rivas A. (2005) Pathogenic Acanthamoeba strains from water sources in Jamaica, West Indies. Ann. Trop. Med. & Parasitol. 99: 751–758
• Lorenzo-Morales J., Maciver S. K. (2006) Long-term, low maintenance storage of Acanthamoeba strains. Parasitology Research 99: 743–745
• Lorenzo-Morales J., Khan N. A., Walochnik J. (2015) An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment. Parasite 22: 10–20
• Maciver S. K., De Obeso Fernandez Del Valle A., Koutsogiannis Z. (2017) Vannella pentlandii n. sp., (Amoebozoa, Discosea, Vannellida) a small, cyst-forming soil amoeba. Exp. Parasitol. (in press)
• Mohamed M. E., Huseein E. A., Farrag H. M., Mostafa F. A. A., Hassan A. T. (2016) Allovahlkampfia spelaea is a potential environmental host for pathogenic bacteria. J. Bacteriol. Parasitol. 7: 255–262
• Niyyati M., Lorenzo-Morales J., Rezaie S., Rahimi F., Martín-Navarro C. M., Mohebali M., Rezaeian M. (2010) First report of a mixed infection due to Acanthamoeba genotype T3 and Vahlkampfia in a cosmetic soft contact lens wearer in Iran. Exp. Parasitol. 126: 89–90
• Olive L. S. (1962) The genus Protostelium. Amer. J. Botany 49: 297–303
• Ozkoc S., Tuncay S., Delibas S. B., Akisu C., Ozbek Z., Durak I., Walochnik J. (2008) Identification of Acanthamoeba genotype T4 and Paravahlkampfia sp. from two clinical samples. J. Med. Microbiol. 57: 392–396
• Page F. C. (1976) An illustrated key to freshwater and soil amoebae. Freshwater Biological Association, Ambleside, England.
• Page F. C. (1978) Acrasis rosea and the possible relationship between Acrasida and Schizopyrenida. Archiv für Protistenkunde. 120: 169–181
• Page F. C., Blanton R. L. (1985) The Heterolobosea (Sarcodina:Rhizopoda), a new class uniting the Schizopyrenida and the Acrasidae (Acrasida). Protistologica 21: 121–132
• Pánek T., Čepička I. (2016) Diversity of Heterolobosea. In “Genetic Diversity of Microorganisms”, 3–26. Eds. Caliskan M., Rijeka M : InTech.
• Pánek T., Simpson A. G. B., Brown M. W., Dyer B. D. (2017) Heterolobosea in “Handboook of the Protists” Archibald et al., eds Springer Int. Publ.
• Perkins D. L., Jahn T. L. (1970) Amoeboflagellate transformations and the Gibbs-Donnan ratio. J. Protozool. 17: 168–172
• Preston T. M. (2003) The water-air interface: a microhabitat for amoebae. Eur. J. Protistol. 39: 385–389
• Purslow C., Wolffsohm J. S., Santodomingo-Rubido J. (2005) The effect of contact lens wear on dynamic ocular surface temperature. Contact Lens & Ant. Eye 28: 29–36
• Radford C. F., Lehmann O. J., Dart J. K. (1998) Acanthamoeba keratitis: multicentre survey in England 1992–1996. Br. J. Ophthalmol. 82: 1387–1392
• Roger A. J., Smith M. W., Doolittle R. F., Doolittle W. F. (1996) Evidence for the heterolobosea from phylogenetic analysis of genes encoding glyceraldehyde-3-phosphate dehydrogenase. J. Euk. Microbiol. 43: 475–485
• Shadwick L. L., Brown M. W., Tice A. K., Spiegel F. W. (2016) A new amoeba with Protosteloid Fruiting: Luapeleamoeba hula n. g. n. sp. (Acanthamoebidae, Centramoebida, Amoebozoa). Acta Protozool. 55: 123–134
• Shutt T. E. (2006) Origins and evolution of mitochondrial transcription and replication machinery. Ph.D. Thesis, Dalhousie University, Halifax, Nova Scotia.
• Siddiqui R., Ali K. M., Cope J. R., Khan N. A. (2016) Biology and pathogenesis of Naegleria fowleri. Acta Tropica 164: 375–394
• Tice A. K., Shadwick L. L., Fiore-Donno A. M., Geisen S., Kang S.,Schuler G. A.,Spiegel F. W., Wilkinson K. A., Bonkowski M., Dumack K., Lahr D. J. G., Voelcker E., Clauß S., Zhang J. Brown M. W. (2016) Expansion of the molecular and morphological diversity of Acanthamoebidae (Centramoebida, Amoebozoa) and identification of a novel life cycle type within the group. Biology Direct 11: 69; doi 10.1186/s13062-016-0171-0
• Tolba M. E. M., Huseein E. A. M., Farrag H. M. M., Mohamed H. E. D., Kobayashi S., Suzuki J., Sugano S. (2016) Allovahlkampfia spelaea causing keratitis in humans. PLoS Negl. Trop. Dis. 10: e0004841
• Tyml T., Skulinová K., Kavan J., Ditrich O., Kostka M., Dyková I. (2016) Heterolobosean amoebae from Arctic and Antarctic extremes: 18 novel strains of Allovahlkampfia, Vahlkampfia and Naegleria. Eur. J. Protistol. 56: 119–133
• Tyml T., Lares-Jiménez L. F., Kostka M., Dyková I. (2017) Neovahlkampfia nana n. sp. Reinforcing an underrepresented subclade of Tetramitia, Heterolobosea. J. Euk. Microbiol. 64: 78–87
• Walochnik J., Mulec, J. (2009) Free-living amoebae in carbonate precipitating microhabitats of karst caves and a new vahlkampfiid amoeba, Allovahlkampfia spelaea gen. nov., sp. nov. Acta Protozool. 48: 25–33

Identifiers

DOI

10.4467/16890027AP.17.016.7497

Biblioteka Nauki

52396528