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2017 | 56 | 2 | 109-118

Article title

Improvement of Silver Impregnation Technique Using in Situ Synthesized Protargol

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Abstracts

EN
An improved impregnation method for ciliates has been described with a new formulation for silver proteinate synthesized in situ, that avoids necessary its long-time laboratory synthesis or use of expensive commercial protargol. Compared to conventional techniques, the proposed protocol is more time-saving, reduces the consumption of chemicals and excludes some hazardous ones (e.g. xylene). Structures that are impregnated such as nuclear apparatus, infraciliature, cortical and cytoplasmic microtubules are stained almost identical compared to other protargol methods. Advantages of this method allow us to merge it successfully with ecological quantitative studies of various natural communities of ciliates and provide correct identification of species during such investigations.

Year

Volume

56

Issue

2

Pages

109-118

Physical description

Dates

published
2017

Contributors

  • Institute of Marine Biology NAS of Ukraine, Odessa, Ukraine

References

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Document Type

Publication order reference

Identifiers

Biblioteka Nauki
52376318

YADDA identifier

bwmeta1.element.ojs-doi-10_4467_16890027AP_17_009_7484
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