Leukocyte Populations Detection in Young Athletes in Resting Phase based on Scatter Properties using a Flow Cytometric Approach

• Authors: Ornela Marko , Klotilda Vrenjo , Majlind Sulçe

Abstracts

EN

Complete blood count analysis has a major importance as a first laboratory evaluation. In particular data obtained from complete blood count are relevant to correctly diagnose and follow up different medical conditions. In athletes, medical examinations and laboratory test are mandatory, especially when a sportive event will soon take place. Different automatic techniques are used to evaluate percentages of different leukocyte populations with a high accuracy. Flow cytometry represents a technology that is mainly used to diagnose different lymphoproliferative and myeloproliferative neoplastic disorders. In this study 127 samples from clinically healthy young athletes were collected and analyzed with both flow cytometry and automatic method to evaluate the usefulness of flow cytometry in detecting different leukocyte populations based on their scatter properties. Results showed that flow cytometry is a reliable technique having showed a high accuracy for different cell populations. Intra class correlation coefficient was >0.80 for all populations showing a high correlation between the two methods. However, a higher number of cases, the involvement of other automatic techniques are mandatory to confirm these results.

Keywords

EN

flow cytometry; leukocytes; populations; athletes

• Publisher: Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
• Journal: Central European Journal of Sport Sciences and Medicine
• Year: 2024
• Volume: 47
• Pages: 29-35

Dates

published

2024

Contributors

author

Ornela Marko

• Department of Movement and Health, Faculty of Physical Activity and Recreation Sports University of Tirana, Rruga Muhamet Gjollesha, 1023, Tirana, Albania

author

Klotilda Vrenjo

• Department of Education and Health, Faculty of Movement Sciences, Sports University of Tirana, Rruga Muhamet Gjollesha, 1023, Tirana, Albania

author

Majlind Sulçe

• Department of Morphofunctional Modules, Faculty of Veterinary Medicine, Agricultural University of Tirana, Albania

• https://orcid.org/0000000188272876

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Identifiers

DOI

10.18276/cej.2024.3-03

Biblioteka Nauki

55790409