The paper presents the results of the study connected with determining the contents of total organic carbon (TOC), total nitrogen (TN), available phosphorus (PE-R), sulphate sulphur (S-SO4 2-), and the activity of acid phosphatase (AcP) and arylsulphatase (ARS) in Haplic Luvisol (LVh) fertilised with varied farmyard manure (FYM) doses (0, 20, 40, 60, 80 t ha-1) and nitrogen in the form of ammonium nitrate (N0, N1, N2, N3). The experiment involved a 4-year crop-rotation (2005-2009). A significant effect of fertilisation with farmyard manure (FYM), ammonium sulphate and the selection of crops in crop rotation on the content of TOC and TN was found. The content of phosphorus available to plants was significantly higher in the soil sampled in the third year of research under spring barley fertilised with the FYM at the dose of 60 t ha-1 and nitrogen at the dose of N2. The activity of acid phosphatase in soil increased with increasing nitrogen doses. The mean amount of sulphates classifies the soil as representing a very high richness and guarantees a good supply of plants with that nutrient. The greatest activity of arylsulphatase was reported in the samples of the soil fertilised with the FYM at the amount of 80 kg ha-1.