Preferences help
enabled [disable] Abstract
Number of results
2015 | 70 | 1 |
Article title

Chlorophyll fluorescence response of wheat to exogenous application of growth regulators under terminal drought stress

Title variants
Languages of publication
Drought stress negatively affects plant photosynthesis and disturbs the electron transport activity. Evaluation of the chlorophyll fluorescence parameters might reflect influence of the environmental stress on plants and can be applied as an indicator of the primary photochemistry of photosynthesis. In current study the effect of foliar application of benzylaminopurine (BAP, a synthetic cytokinin) and abscisic acid (ABA) on chlorophyll fluorescence parameters of relatively drought tolerant (Pishtaz) and susceptible (Karaj3) bread wheat genotypes under well watered and terminal water deficit condition have been evaluated. Terminal drought was induced by withholding water at anthesis stage (Zadoks scale 65). Results showed that coefficient of non-photochemical quenching of variable fluorescence (qN), quantum yield of PS II photochemistry (ΦPSII) and photochemical quenching (qP) were affected by hormone spray treatments. So that evaluation of parameters at 7 day after foliar treatments revealed that ABA significantly increased electron transport rate (ETR) and qN while considerably decreased ΦPSII, gs and maximum quantum yield of photosystem II (Fv/Fm). However exogenous application of cytokinin could increase gs, Fv/Fm and ΦPSII and the highest value of these parameters was recorded in cytokinin treated plants of Pishtaze cv. under well watered condition. Nevertheless, evaluation of the parameters in different periods after spraying showed that with approaching the maturity stage some traits like as gs, Fv/Fm and ETR significantly decreased in both genotypes. Evaluation of gs and Chlorophyll fluorescence parameters of genotypes between different irrigation levels showed that although cv. Pishtaz showed higher performance of PSII under well watered condition, it failed to maintain its superiority under stress condition. This finding suggests that some more responsive parameter like gs, Fv/Fm and ΦPSII can be considered as reliable indicator for understanding the biochemical and physiological effects of exogenous application of phytohormones under terminal drought stress.
Stres suszy wpływa negatywnie na fotosyntezę roślin oraz zakłóca transport elektronów. Ocena parametrów fluorescencji chlorofilu może odzwierciedlać wpływ stresu środowiskowego na rośliny i może być stosowana jako wskaźnik pierwotnych reakcji fotochemicznych fotosyntezy. W prezentowanych badaniach oceniano wpływ dolistnego stosowania benzyloaminopuryny (BAP, syntetyczna cytokinina) i kwasu abscysynowego (ABA) na parametry fluorescencji chlorofilu w stosunkowo odpornych na suszę (Pishtaz) i podatnych (Karaj3) genotypach pszenicy w warunkach dobrego nawodnienia i skrajnego deficytu wody. Krańcową suszę wywoływano w fazie kwitnienia (65 w skali Zadoks). Wykazano, że współczynnik niefotochemicznego wygaszania fluorescencji (qN), wydajność kwantowa reakcji fotochemicznych PS II (ΦPSII) i fotochemiczne wygaszanie (qP) ulegały zmianom po opryskiwaniu liści hormonami. Ocena parametrów po 7 dniach od dolistnego podania hormonu wykazała, że ABA znacząco zwiększa sprawność transportu elektronów (ETR) i qN, znacznie zmniejsza ΦPSII, gs i maksymalną wydajność kwantową fotosystemu II (Fv/Fm). Jednak egzogenne zastosowanie cytokininy może zwiększyć gs, Fv/Fm i ΦPSII, a najwyższą wartość tych parametrów odnotowano w traktowanych cytokininą roślinach odmiany Pishtaze w warunkach dobrego nawodnienia. Ocena parametrów w różnych okresach po oprysku wykazała, że w bardziej dojrzałych roślinach wskaźniki takie jak gs, Fv/Fm i ETR znacznie zmniejszyły się w obu genotypach. Ocena gs i parametrów fluorescencji chlorofilu genotypów w warunkach różnych poziomów nawodnienia wykazała, że chociaż genotypy Pishtaz wykazały wyższą wydajność PSII w stanie dobrego nawodnienia, nie udało się utrzymać przewagi w warunkach stresu. Odkrycie to sugeruje, że niektóre bardziej czułe parametry takie jak gs, Fv/Fm i ΦPSII mogą być wiarygodnym wskaźnikiem dla zrozumienia biochemicznych i fizjologicznych efektów egzogennego stosowania fitohormonów w warunkach krańcowego stresu suszy.
Physical description
07 - 09 - 2015
  • Ali Z., Basra S. M. A., Munir H., Mahmood A., Yousaf S. (2011): Mitigation of drought stress in maize by natural and synthetic growth promoters. Journal of Agriculture and Social Sciences, 7 (2): 56-62.
  • Ashraf M., Harris, P. J. C. (2013): Photosynthesis under stressful environments: an overview. Photosynthetica, 51(2): 163-190.
  • Bahar B., Yildirim M., Barutcular C. (2009): Relationships between stomatal conductance and yield components in spring durum wheat under Mediterranean conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37 (2): 45-48.
  • Balouchi H.R. (2010): Screening wheat parents of mapping population for heat and drought tolerance, detection of wheat genetic variation. International Journal of Biological Sciences, 6: 56–66.
  • Bota J., Medrano H., Flexas J. (2004): Is photosynthesis limited by decreased Rubisco activity and RuBP content under progressive water stress?. New phytologist, 162 (3): 671–681.
  • Brestic M., Zivcak M.(2013): PSII fluorescence techniques for measurement of drought and high temperature stress signal in crop plants: protocols and applications. Rout G. R., Das A. B. (eds.). Molecular Stress Physiology of Plants, Springer Dordrecht, pp. 87–131.
  • Brodribb T. J., McAdam S. A. (2013): Abscisic acid mediates a divergence in the drought response of two conifers. Plant physiology, 162 (3): 1370–1377.
  • Cochard H., Coll L., Roux X. L., Amglio T. (2002): Unraveling the Effects of Plant Hydraulics on Stomata1 Closure during Water Stress in Walnut. Plant Physiology, 128: 282–290.
  • Damour G., Simonneau T., Cochard H., Urban L. (2010): An overview of models of stomatal conductance at the leaf level. Plant Cell Environ, 33: 1419-1438.
  • Dani K. G. S., Jamie I. M., Prentice I. C., Atwell B. J. (2014): Increased ratio of electron transport to net assimilation rate supports elevated isoprenoid emission rate in eucalypts under drought. Plant Physiology, 166 (2): 1059–1072.
  • FAOSTAT (2012): FAO STAT Data of Food and Agriculture Organization of the United Nations,
  • Falk S., Maxwell D.P., Laudenbach D.E., Huner N.P.A., Baker N. R. (1996): In Advances in Photosynthesis, V.5, Photo-synthesis and the Environment (ed.), Kluwer Academic Publishers, Dordrecht Boston London. pp. 367–385.
  • Fernández-Cirelli A., Arumí J. L., Rivera D., Boochs, P. W. (2009): Environmental effects of irrigation in arid and semi-arid regions. Chilean Journal of Agricultural Research, 69: 27–40.
  • Franks P. J. (2013): Passive and active stomatal control: either or both?. New Phytologist, 198 (2): 325–327.
  • Genty B., Briantais J. M., Baker N. R. (1989): The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta (BBA)-General Subjects, 990 (1): 87–92.
  • Haisel D., Pospíšilová J., Synková H., Schnablová R., Baťková P. (2006): Effects of abscisic acid or benzyladenine on pigment contents, chlorophyll fluorescence, and chloroplast ultrastructure during water stress and after rehydration. Photosynthetica, 44 (4): 606-–614.
  • Jia H., Lu C. (2003): Effects of abscisic acid on photoinhibition in maize plants. Plant science, 165 (6): 1403–1410.
  • Jiang Q., Roche D., Monaco T. A., Hole, D. (2006): Stomatal conductance is a key parameter to assess limitations to photosynthesis and growth potential in barley genotypes. Plant Biology, 8 (4): 515–521.
  • Li R. H., Guo P. G., Michael B., Stefania G., Salvatore C. (2006): Evaluation of chlorophyll content and fluorescence parameters as indicators of drought tolerance in barley. Agricultural Sciences in China, 5 (10): 751–757.
  • Ma, Q. Q., Wang, W., Li, Y. H., Li, D. Q., & Zou, Q. (2006):. Alleviation of photoinhibition in drought-stressed wheat (Triticum aestivum) by foliar-applied glycinebetaine. Journal of Plant Physiology, 163 (2), 165–175.
  • Misra A. N., Misra M., Singh, R. (2012): Chlorophyll fluorescence in plant biology. INTECH Open Access Publisher.
  • Murata N., Takahashi S., Nishiyama Y., Allakhverdiev S. I. (2007): Photoinhibition of photosystem II under environmental stress. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1767 (6): 414–421.
  • Nachabe M. H. (1998): Refining the definition of field capacity in the literature. Journal of irrigation and drainage engineering, 124 (4): 230–232.
  • Prokopová J., Špundová M., Sedlářová M., Husičková A., Novotný R., Doležal K., Lebeda A. (2010): Photosynthetic responses of lettuce to downy mildew infection and cytokinin treatment. Plant Physiology and Biochemistry, 48 (8): 716–723.
  • Sarafraz-Ardakani M. R., Khavari-Nejad R. A., Moradi F., Najafi F. (2014): Abscisic acid and cytokinin-induced osmotic and antioxidant regulation in two drought-tolerant and drought-sensitive cultivars of wheat during grain filling under water deficit in field conditions. Notulae Scientia Biologicae, 6 (3): 354–362.
  • Vlčková A., Špundová M., Kotabová E., Novotný R., Doležal K., Nauš, J. (2006): Protective cytokinin action switches to damaging during senescence of detached wheat leaves in continuous light. Physiologia Plantarum, 126 (2): 257–267.
  • Yang J., Zhang J., Wang Z., Zhu Q. (2003): Hormones in the grains in relation to sink strength and postanthesis development of spikelets in rice. Plant Growth Regulation, 41 (3): 185–195.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.