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2018 | 14 | 23-32

Article title

The use of in vitro assays for the assessment of cytotoxicity on the example of MTT test

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Languages of publication

EN

Abstracts

EN
In recent years, biological tests have been developed based on cell cultures and successfully used to the hygienic assess of a variety of samples. In vitro assays become the complement of conventional chemical methods. They do not narrow the results only to the quantitative and qualitative information on toxic substances, but also increase knowledge on the direct impact on the organism. They are also an alternative for animal testing, which are currently given up for ethical reasons. At present, the market is steadily increasing in the number of tests and bio-assay techniques. Based on our own studies we conclude that the MTT test is perfect as a diagnostic method for evaluating the cytotoxicity of materials of different composition such as mycotoxins, pesticides, bacterial cultures, moulds isolates, food, feed, as well as a vast spectrum of other environmental samples.
PL
Testy biologiczne oparte na hodowlach komórkowych z powodzeniem stosowane są do oceny cytotoksyczności leków, artykułów spożywczych, pasz. Testy in vitro stają się uzupełnieniem konwencjonalnych metod chemicznych, a także alternatywą dla badań przeprowadzanych na zwierzętach. Na podstawie badań własnych można stwierdzić, że test MTT jest czułą metodą diagnostyczną przydatną w ocenie cytotoksyczności nie tylko mikotoksyn, pestycydów, bakterii, pleśni, żywności, pasz, ale także próbek uzyskanych z materiałów budowlanych.

Keywords

Year

Volume

14

Pages

23-32

Physical description

Dates

published
2018-12-30

Contributors

References

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.ojs-doi-10_1515_fobio-2017-0006
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