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1998 | 13 |
Article title

Badanie oddziaływania produktów degradacji Paraquatu i Gramoxonu z hemoglobiną człowieka i ryby

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Title variants
PL
Studies on degradation products of Paraquat and Gramoxone interactions with hum an and carp hemoglobins
Languages of publication
Abstracts
EN
In this work the effect of Paraquat (PQ) and Graraoxone (GX) on isolated human and
carp hemoglobins was studied. Degradation products of PQ were isolated from GX solution
by thin layer chromatography on silica gel platelets. Purity of the GX fractions obtained was
verified on the basis of silica gel rechromatography. The fractions were identified by IR, UV
and visible spectra. Binding of PQ degradation products to hemoglobin was studied by
molecular filtration through a Sephadex G-50 column. Interaction of GX fractions with
hemoglobin was studied by the spectrophotometric method. Absorption spectra were recorded
in the X = 200-900 nm range in 30-min intervals, between 0 and 3 hrs of incubation. Effect
of pH of the medium on the amount of hemoglobin-bound ligand was determined, and the
reactivity of the GX fractions obtained was compared with those of PQ and GX. Conditions
of the strongest and the weakest binding of the ligands were identified. The studies performed
point to a considerable importance of Hb in the transport of PQ and its degradation products
to various tissues. The stronger binding of these ligands by carp Hb confirms that fishes are
more sensitive to poisoning by bipyridyl derivatives as compared with mammals (primates).
Keywords
Year
Volume
13
Physical description
Dates
published
1987
References
Document Type
Publication order reference
Identifiers
URI
http://hdl.handle.net/11089/14574
YADDA identifier
bwmeta1.element.hdl_11089_14574
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