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The cytometric method for measurement of the viability of lymphoid cells under heat shock
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Abstracts
The viability of freshly isolated or heat-treated lymphocytes was estimated by the classical
microscopic method based on trypan blue inclusion and it was measured by flow cytometry of the
cells stained with acridine orange and ethydium bromide or with acridine orange alone. It has been
showed that (low cytometry of fluorescence stained cells allowed to determine the viability of the
cell suspensions containing even a small percentage of dead cells rapidly and accurately.
microscopic method based on trypan blue inclusion and it was measured by flow cytometry of the
cells stained with acridine orange and ethydium bromide or with acridine orange alone. It has been
showed that (low cytometry of fluorescence stained cells allowed to determine the viability of the
cell suspensions containing even a small percentage of dead cells rapidly and accurately.
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Dates
published
1994
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References
Document Type
Publication order reference
Identifiers
URI
http://hdl.handle.net/11089/14090
YADDA identifier
bwmeta1.element.hdl_11089_14090
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