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Title variants
Influence of blood conservation on nucleic acids content and activity of nucleolytic enzymes in human peripheral blood lymphocytes
Languages of publication
Abstracts
The lymphocytes from human peripheral blood of healthy donors stored from
4°C to 6°C in three different conserving fluids of the following composition:
1) ACDf.B: 0.045 M trisodium citrate, 0.025 M citric acid, 0.035 M glucose,
pH = 4.9; 2) CPD: 0.088 M trisodium citrate, 0.015 M citric acid 0.139 M glucose, 0.158 M sodium phosphate, pH - 5.63; 3) CPD with the addition of adeni ne and adenosine, were used as the material for investigations. The conservation
time of blood was 0, 7, 14, 21 days for ACDf.B; 0, 7, 14, 21, 28 days for
CPD and 0, 7, 14, 21, 28, 42 days for CPD with adenine and adenosine. The contents
of RNA and DNA were determined by spectrophotometry method of Markov and
Tsanev, the activity of ribonuclease by the method of Frih-Niggemayer-Reddi,
deoxyribonuclease by the method of Rotherham and acid phosphatase by King-Armstrong’s method.
Some changes were found in the DNA and RNA contents and in the activity of
Investigated enzymes, depending on the time and conditions of blood conservation.
These changes resemble the processes observed in mitogen stimulated lymphoyctes.
4°C to 6°C in three different conserving fluids of the following composition:
1) ACDf.B: 0.045 M trisodium citrate, 0.025 M citric acid, 0.035 M glucose,
pH = 4.9; 2) CPD: 0.088 M trisodium citrate, 0.015 M citric acid 0.139 M glucose, 0.158 M sodium phosphate, pH - 5.63; 3) CPD with the addition of adeni ne and adenosine, were used as the material for investigations. The conservation
time of blood was 0, 7, 14, 21 days for ACDf.B; 0, 7, 14, 21, 28 days for
CPD and 0, 7, 14, 21, 28, 42 days for CPD with adenine and adenosine. The contents
of RNA and DNA were determined by spectrophotometry method of Markov and
Tsanev, the activity of ribonuclease by the method of Frih-Niggemayer-Reddi,
deoxyribonuclease by the method of Rotherham and acid phosphatase by King-Armstrong’s method.
Some changes were found in the DNA and RNA contents and in the activity of
Investigated enzymes, depending on the time and conditions of blood conservation.
These changes resemble the processes observed in mitogen stimulated lymphoyctes.
Keywords
Publisher
Year
Volume
Physical description
Dates
published
1988
Contributors
References
Document Type
Publication order reference
Identifiers
URI
http://hdl.handle.net/11089/13786
YADDA identifier
bwmeta1.element.hdl_11089_13786
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