B-cell chronic lymphocytic leukemia-associated nuclear antigens

• Authors: Małgorzata Rogalińska , Jerzy Z Błoński , Tadeusz Robak , Zofia M Kiliańska

Abstracts

EN

One- and two-dimensional polyacrylamide gel electrophoresis were used to compare
the composition of nuclear polypeptides from normal and В-cell chronic lymphocytic leukemia mononuclear cells. Against two electrophoretically-specific nuclear proteins with molecular weight
of 38/39 and 44/46 kD a from leukemic cells rabbit sera were obtained. As it was analyzed by Western blot technique the available antisera recognized the 38/39 kDa antigen in 53 of the 56
(94.6%), while the 44/46 kDa in 46 of the 49 (93.9%) of examined В-CLL nuclear fraction
preparations, but not in normal ones. The pi values of described leukaemia-specific antigens were
determined; p38/39 had pi in the range of pH 6.55 -7.00 and p44/46 - in the range of pH 6.2-6.4.

Keywords

EN

B-CLL specific antigens; electrophoresis; immunoblotting

• Publisher: Wydawnictwo Uniwersytetu Łódzkiego
• Journal: Acta Universitatis Lodziensis. Folia Biologica et Oecologica
• Year: 2008
• Volume: 4

Dates

published

2008

Contributors

author

Małgorzata Rogalińska

author

Jerzy Z Błoński

author

Tadeusz Robak

author

Zofia M Kiliańska

other

Department of Cytobiochemistry Małgorzata Rogalińska - University of Łódź

other

Department of Hematology Jerzy Z. Błoński - Medical University of Łódź

other

Department of Hematology Tadeusz Robak - Medical University of Łódź

other

Department of Cytobiochemistry Zofia M. Kiliańska - University of Łódź

Identifiers

URI

http://hdl.handle.net/11089/12336